Effect of Pretreatment with DPI and H2O2 on the Antioxidant System of Sweetpotato Seedlings in a Potassium Deficient Environment

doi:10.7668/hbnxb.20194278

Abstract

Abstract:

In order to investigate the effects of reactive oxygen species(ROS)on the growth and antioxidant system of sweetpotato under potassium deficiency stress,and further reveal the defense mechanism of ROS regulation in sweetpotato under potassium deficiency stress.This study selected two sweetpotato varieties,Xushu32(low potassium tolerant)and Ningzi 1(low potassium sensitive),as experimental materials.The experiment was conducted in an artificial climate chamber using hydroponic cultivation to ensure consistent environmental conditions.After pretreatment with exogenous H₂O₂ and diphenyl chloroiodate(DPI),ROS inhibitor,the sweetpotato seedlings were subjected to potassium deficiency treatment.The changes in relative H₂O₂ content,antioxidant gene expression levels,and enzyme activity indicators in sweetpotato roots at different time points after potassium deficiency treatment were measured.The morphological characteristics of sweetpotato were recorded to explore the effects of ROS signaling on the growth of sweetpotato seedlings under potassium deficiency stress.The results showed that under potassium deficiency treatment,the biomass of sweetpotato decreased and leaf color lightened.The relative concentration of ROS in sweetpotato seedling roots showed the highest fluorescence intensity on the 14th day.The expression levels of antioxidant-related genes in sweetpotato roots generally increased,with a more significant increase in expression levels after H₂O₂ pretreatment.Compared with-K treatment,both H₂O₂ and DPI pretreatment could enhance the activity of antioxidant enzymes in sweetpotato leaves,and different enzyme activities showed different trends within 21 days of potassium deficiency treatment.There were varietal differences in the effects of different treatments.The experimental results showed that H₂O₂ could act as a stress signaling molecule to increase the expression level of antioxidant genes and the enzymatic activities of APX,SOD and POD in sweetpotato seedlings,thus scavenging excess ROS and effectively alleviating the inhibition of growth of sweetpotato seedlings by potassium deficiency stress.And DPI could inhibit the production of endogenous H₂O₂,maintain the antioxidant enzyme activity,protect the ROS enzymatic scavenging system of sweetpotato seedlings and avoid oxidative damage to tissues.

Key words: Sweetpotato, Potassium deficiency stress, H₂O₂, ROS, DPI

GUO Pengyu, ZHANG Qiangqiang, JIN Rong, ZHAO Peng, WANG Jing, ZHU Xiaoya, YU Yongchao, LIU Ming, WU Dexiang, TANG Zhonghou. Effect of Pretreatment with DPI and H₂O₂ on the Antioxidant System of Sweetpotato Seedlings in a Potassium Deficient Environment[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 108-116. doi: 10.7668/hbnxb.20194278.

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Figures/Tables 6

Tab.1 Primers used in this study

基因名 Gene name	引物 Primers	序列(5'-3') Sequence (5'-3')	碱基数 Bases
IbARF	IbARF-F	CTTTGCCAAGAAGGAGATGC	20
	IbARF-R	TCTTGTCCTGACCACCAACA	20
IbAPX1	IbAPX1-F	TGGCACTCTGCTGGAACT	18
	IbAPX1-R	GCATAAGAAACAATAGGGA	19
IbCuZnSOD	IbCuZnSOD-F	GGGTGTCGCAGTTCTTAG	18
	IbCuZnSOD-R	ATTGAAATGTGGTCCAGTAG	20
IbPOD	IbPOD-F	TCTTCTTCCACGATTGCTT	19
	IbPOD-R	AGTCACAGTGTATGTTGCTCC	21
IbRbohF	IbRbohF-F	TTGGAAAGAGTGGGTTGC	18
	IbRbohF-R	CAGGATACTAATAAAGGGTGT	21

Fig.1 Phenotypic differences of Xu32 and Nz1 under different treatments at 21 d

Fig.2 Qualitative determination of the relative ROS content of Xu32 and Nz1 roots among different treatments in 21 d

Fig.3 Quantitative determination of the relative ROS content of Xu32 and Nz1 roots among different treatments in 21 d For the same time,different small letters above bars indicate significant difference among treatments at P<0.05.The same as Fig.4-5.

Fig.4 Effect of different treatments on RT-PCR expression of antioxidant-related genes in Xu32 and Nz1 roots during 21 d

Fig.5 Effects of different treatments on the activities of antioxidant-related enzymes in Xu32 and Nz1 roots during 21 d

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