Transcriptomic Analysis of Maize Silks in Response to Drought Stress

doi:10.7668/hbnxb.20194627

Abstract

Abstract:

To investigate the molecular response to drought stress of silks,two treatments with soil moisture content of 80% field holding capacity (CK) and 60% field holding capacity (DS) were set up using Annong 591 and Xianyu 335,and the maize silks were sampled during the rapid increase in silk elongation,and transcriptome sequencing was performed.By comparing the gene expression differences between the drought and control groups of the two varieties,the key pathways and related candidate genes of maize silks in response to drought were clarified.The results showed that the phenylpropane biosynthesis pathway (ko00940) and starch and sucrose metabolism pathway (ko00500) were the key pathways in maize silk response to drought.In the phenylpropane biosynthetic pathway 4CL,CCR,CAD and POD genes were lowly expressed in drought-prone maize silks.Correlation analysis showed that down-regulation of 4CL,CCR,CAD and POD genes was associated with reduced lignin content.Converting enzyme and sucrose synthase genes in the starch and sucrose metabolic pathways were lowly expressed in drought maize silks.Correlation analysis indicated that down-regulation of convertase and sucrose synthase genes was associated with sucrose metabolism.In summary,this study identified key genes and metabolites involved in sucrose and lignin metabolism.The lignin synthesis are associated with drought tolerance in maize silks,and 4CL, CCR,CAD,and POD are the candidate genes for drought tolerance in maize silks.In addition,the starch and sucrose metabolic pathways are associated with silk elongation and drought tolerance of silks,and invertase and sucrose synthase are the main candidate key enzymes.

Key words: Maize, Silks, Drought stress, Transcriptome, Phenylpropanoid biosynthesis, Starch and sucrose metabolism

ZHU Fan, XU Zhiqi, DUAN Yujie, LI Yangyang, SONG Youhong. Transcriptomic Analysis of Maize Silks in Response to Drought Stress[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 37-47. doi: 10.7668/hbnxb.20194627.

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Figures/Tables 8

Tab.1 The average elongation rate of ASI and filaments in the control group (CK) and drought group (DS) of AN591 and XY335

品种 Variety	处理 Treatment	开花吐丝间隔期/d ASI	花丝平均伸长速率 (cm/(℃·d)) Average filament elongation rate
安农591	CK	1	0.10
AN591	DS	2	0.08
先玉335	CK	1	0.10
XY335	DS	3	0.07

Fig.1 Biological repeated parallelism test A.PCA principal component analysis;B.Sample correlation analysis.

Fig.2 Statistics on the number of differential genes between AN591 and XY335

Fig.3 KEGG annotation between AN591 and XY335 differentially expressed genes under drought treatment

Fig.4 AN591 and XY335 annotated to differentially expressed genes in the KEGG pathway A.Phenylpropanoid biosynthesis in AN591;B.Phenylpropanoid biosynthesis in XY335;C.Starch and sucrose metabolism in AN591;D.Starch and sucrose metabolism in XY335.The graphs are marked in green for down-regulation of gene expression following drought stress;in blue for both up-and down-regulation of gene expression following drought stress;in red for up-regulation of gene expression following drought stress.The same as Fig.5.

Fig.5 Heat map of clustering of up-and down-regulated differentially expressed genes in AN591 and XY335

Fig.6 Average POD activity of AN591 and XY335 control and drought groups Different lowercase letters indicate significant differences under different treatments(P<0.05).

Tab.2 Invertase activity of AN591 and XY335 control and drought groups

指标 Index	处理 Treatments	品种Variety
指标 Index	处理 Treatments	安农591 AN591	先玉335 XY335
细胞壁转化酶/(U/g)	CK	584.94±18.18a	544.72±32.61a
CWIN	DS	450.55±27.59b	519.00±19.43a
酸性转化酶/(μg/(min·g))	CK	493.93±55.76b	438.62±39.80a
VIN	DS	552.98±89.27ab	497.69±50.77a
中性转化酶/(U/g)	CK	193.15±29.88a	133.47±6.56b
NI	DS	150.95±10.26a	229.86±5.57a

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