Acta Agriculturae Boreali-Sinica ›› 2024, Vol. 39 ›› Issue (1): 72-82. doi: 10.7668/hbnxb.20194000

Special Issue: Chestnut Drought and water saving Biotechnology Fruits

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology • Previous Articles     Next Articles

Identification and Expression Analysis of the Chestnut WRKY Gene Family under Drought Stress

ZHAO Shuang1,2, GE Chaohong1, SHI Hefei2, MIN Zhuo1, WANG Guangpeng3, LI Weiming1   

  1. 1 Institute of Cotton,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050000,China
    2 Cangzhou Technical College,Cangzhou 061000,China
    3 Changli Institute of Pomology, Hebei Academy of Agriculture and Forestry Sciences,Changli 066600,China
  • Received:2023-10-02 Published:2024-03-03

Abstract:

WRKY transcription factors play an important role in the regulation of plant growth and defence responses.In order to explore the function of WRKY gene family in chestnut under drought resistance,the chestnut WRKY gene family members were identified and the physical and chemical properties,phylogeny and structure of the encoded proteins were analyzed.Transcriptome sequencing and qRT-PCR were used to analyze the expression characteristics of WRKY gene family members under drought stress.The results showed that 65 WRKY gene family members were identified and divided into three groups:Ⅰ,Ⅱ and Ⅲ,among which group Ⅱ was divided into five subgroups according to its structure and phylogenetic relationship.Analysis of WRKY gene structure and conserved motif showed that the number of exons ranged from 1 to 7,and the number of exons and motif distribution in the same subgroup were similar.The conserved domain of WRKY gene had certain variation,including the deletion of WRKY heptapeptide domain,the deletion of zinc finger structure,and the heptapeptide domain was mutated into WRKYGKK,WRKYGRK and WRKYGRK.Transcriptome sequencing data showed that 4 WRKY family members were not expressed in the samples,and 49 WRKY family members were up-regulated under drought stress,while 11 WRKY family members were down-regulated under drought stress.The above results showed that,chestnut WRKY genes played an important role in response to drought stress.

Key words: Chestnut, Gene family, WRKY, Drought stress

Cite this article

ZHAO Shuang, GE Chaohong, SHI Hefei, MIN Zhuo, WANG Guangpeng, LI Weiming. Identification and Expression Analysis of the Chestnut WRKY Gene Family under Drought Stress[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 72-82. doi: 10.7668/hbnxb.20194000.

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