Identification of Metacaspase Family Genes in Brassica napus and Analysis of Gene Expression under Drought Stress

doi:10.7668/hbnxb.20195788

Abstract

Abstract:

Metacaspase (MC) belongs to arginine/threonine specific protease,studies have shown that it plays a role in programmed cell death.To investigate the distribution of MC family genes in the genome of Brassica napus and whether they respond to drought stress,this study systematically analyzed the physicochemical properties,phylogeny,gene structure,conserved domains,cis-acting elements,and expression patterns of MC family genes under drought(PEG6000)and abscisic acid(ABA)stress in B.napus.A total of 25 BnMC genes were identified.Chromosomal localization showed that the 25 BnMCs were distributed on 13 chromosomes.Subcellular localization prediction showed that 17 members of the BnMC family were localized in the nucleus and seven members were in the cytoplasm.The phylogenetic tree classified BnMC into two major classes (Type Ⅰ and Type Ⅱ) and four branches (Group A,Group B,Group C,and Group D).BnMCs of the same branch had similar gene structure and conserved motif distribution.The core promoter regions of BnMC contained four types of cis-acting elements:light response element,phytohormone response element,plant growth and development response element and stress response element.Among all the cis-acting elements related to abiotic stress responses,the abscisic acid response element (ABRE) was the most abundant,with a total of 79.All members contained this cis-acting element.The transcriptome sequencing revealed that the expressions of BnMC10,BnMC22,BnMC1,BnMC12 and BnMC8 were up-regulated and the expressions of BnMC4 and BnMC5 were down-regulated after drought treatment.The qRT-PCR assay showed BnMC10,BnMC8,BnMC1 and BnMC12 genes were expressed in both roots and leaves and were up-regulated by both PEG6000 and ABA,with BnMC1 showing the most significant up-regulating changes.In summary,the response of B.napus to drought stress involves the regulation of the expression level of MC family genes.

Key words: Brassica napus, Metacaspase, Gene family, Drought stress

CLC Number:

LI Tian, WANG Daojie, ZHANG Xiaojuan, HOU Yangzi. Identification of Metacaspase Family Genes in Brassica napus and Analysis of Gene Expression under Drought Stress[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(6): 21-30. doi: 10.7668/hbnxb.20195788.

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References 33

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基因名称 Gene name	正向引物序列(5'—3') Forward primer sequence(5'—3')	反向引物序列(5'—3') Reverse primer sequence(5'—3')
BnMC1	CAATCATTTTCCGCCGGCAC	GCAAGACTCGGGGAACTTG
BnMC8	GCACTTCCGGATAATGATGATGAAT	GCTTCATCTATGAGACCACCAC
BnMC10	TACCGTATCCCTAACAAGCAAAAC	GAAGTCCAGAGGACAGAGCGTC
BnMC12	ACGTGCCTTCGTCTTCTTTC	TGATGCACCCTTTCAACTCGT
BnActin	GCTGACCGTATGAGCAAAGA	CACGAACCAGAAGGCAGAAA

基因名称 Gene name	正向引物序列(5'—3') Forward primer sequence(5'—3')	反向引物序列(5'—3') Reverse primer sequence(5'—3')
BnMC1	CAATCATTTTCCGCCGGCAC	GCAAGACTCGGGGAACTTG
BnMC8	GCACTTCCGGATAATGATGATGAAT	GCTTCATCTATGAGACCACCAC
BnMC10	TACCGTATCCCTAACAAGCAAAAC	GAAGTCCAGAGGACAGAGCGTC
BnMC12	ACGTGCCTTCGTCTTCTTTC	TGATGCACCCTTTCAACTCGT
BnActin	GCTGACCGTATGAGCAAAGA	CACGAACCAGAAGGCAGAAA

基因名称 Gene name	参考基因ID Reference gene ID	氨基酸数目/aa Number of amino acids	分子质量/ku Molecular weight	等电点 Isoelectric point	亚细胞定位预测 Prediction of subcellular localization
BnMC1	BnaA01G0146400ZS	447	48.46	6.01	细胞核
BnMC2	BnaA02G0015400ZS	323	35.26	5.88	细胞质
BnMC3	BnaA02G0236900ZS	318	35.30	7.61	细胞核
BnMC4	BnaA02G0407600ZS	380	42.20	7.20	细胞核
BnMC5	BnaA06G0292800ZS	407	45.50	8.84	细胞核
BnMC6	BnaA07G0231500ZS	370	41.37	4.96	细胞质
BnMC7	BnaA07G0381800ZS	277	30.58	8.25	细胞核
BnMC8	BnaA07G0381900ZS	680	74.82	4.35	细胞核
BnMC9	BnaA09G0622400ZS	681	76.28	8.69	细胞核
BnMC10	BnaA09G0677200ZS	604	66.85	8.54	细胞外
BnMC11	BnaA10G0285700ZS	324	35.45	5.62	细胞质
BnMC12	BnaC01G0186200ZS	462	50.08	6.04	细胞核
BnMC13	BnaC02G0015500ZS	332	36.01	5.43	细胞质
BnMC14	BnaC02G0063600ZS	211	23.61	5.07	细胞核
BnMC15	BnaC02G0318400ZS	400	44.33	6.52	细胞核
BnMC16	BnaC02G0541600ZS	389	43.33	7.39	细胞核
BnMC17	BnaC03G0533600ZS	412	45.89	8.72	细胞核
BnMC18	BnaC05G0217400ZS	362	40.26	6.93	细胞核
BnMC19	BnaC05G0217600ZS	120	13.27	7.25	细胞质
BnMC20	BnaC06G0250200ZS	260	29.16	5.11	细胞质
BnMC21	BnaC06G0448900ZS	389	42.99	5.23	细胞核
BnMC22	BnaC06G0449100ZS	706	78.00	4.53	细胞核
BnMC23	BnaC08G0479000ZS	312	34.36	6.18	细胞核
BnMC24	BnaC09G0137300ZS	323	35.47	4.73	细胞核
BnMC25	BnaC09G0604300ZS	322	35.28	5.61	细胞质

基因名称 Gene name	参考基因ID Reference gene ID	氨基酸数目/aa Number of amino acids	分子质量/ku Molecular weight	等电点 Isoelectric point	亚细胞定位预测 Prediction of subcellular localization
BnMC1	BnaA01G0146400ZS	447	48.46	6.01	细胞核
BnMC2	BnaA02G0015400ZS	323	35.26	5.88	细胞质
BnMC3	BnaA02G0236900ZS	318	35.30	7.61	细胞核
BnMC4	BnaA02G0407600ZS	380	42.20	7.20	细胞核
BnMC5	BnaA06G0292800ZS	407	45.50	8.84	细胞核
BnMC6	BnaA07G0231500ZS	370	41.37	4.96	细胞质
BnMC7	BnaA07G0381800ZS	277	30.58	8.25	细胞核
BnMC8	BnaA07G0381900ZS	680	74.82	4.35	细胞核
BnMC9	BnaA09G0622400ZS	681	76.28	8.69	细胞核
BnMC10	BnaA09G0677200ZS	604	66.85	8.54	细胞外
BnMC11	BnaA10G0285700ZS	324	35.45	5.62	细胞质
BnMC12	BnaC01G0186200ZS	462	50.08	6.04	细胞核
BnMC13	BnaC02G0015500ZS	332	36.01	5.43	细胞质
BnMC14	BnaC02G0063600ZS	211	23.61	5.07	细胞核
BnMC15	BnaC02G0318400ZS	400	44.33	6.52	细胞核
BnMC16	BnaC02G0541600ZS	389	43.33	7.39	细胞核
BnMC17	BnaC03G0533600ZS	412	45.89	8.72	细胞核
BnMC18	BnaC05G0217400ZS	362	40.26	6.93	细胞核
BnMC19	BnaC05G0217600ZS	120	13.27	7.25	细胞质
BnMC20	BnaC06G0250200ZS	260	29.16	5.11	细胞质
BnMC21	BnaC06G0448900ZS	389	42.99	5.23	细胞核
BnMC22	BnaC06G0449100ZS	706	78.00	4.53	细胞核
BnMC23	BnaC08G0479000ZS	312	34.36	6.18	细胞核
BnMC24	BnaC09G0137300ZS	323	35.47	4.73	细胞核
BnMC25	BnaC09G0604300ZS	322	35.28	5.61	细胞质

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