Transcriptome Analysis of Low Temperature Response in Peach Leaves

doi:10.7668/hbnxb.20194251

Abstract

Abstract:

Cold resistance is an important target trait for peach breeding and is regulated by multiple genes.Transcriptome sequencing is used to sequence transcripts under specific physiological conditions to explore the molecular mechanism of low temperature response of peach leaves.Leaves of Xiongyuejutao under 4 ℃ low temperature(LT)treatment and normal leaves(RT)were analyzed by transcriptome sequencing to explore the molecular regulation mechanism of cold resistance.A total of 72 up-regulated differentially expressed genes and 3 down-regulated genes were obtained by DESeq2.KEGG analysis was used to analyze the functions of differentially expressed genes in MAPK signaling pathway and plant-pathogen interaction pathway,and protein interaction was predicted.The results showed that leaves under low temperature treatment showed physiological states of curled,yellow,and water loss.The differentially expressed genes were mainly enriched in plant-pathogen interaction,MAPK signaling pathway,and secondary biomass accumulation.MYC2,SPCH and Pti4—6 transcription factors may play an important role in the regulation of cold-induced injury in response to cold stress.MYC2 and CBF proteins were predicted to interact with each other and interacted with multiple proteins.The results showed that multiple metabolic pathways responded to low temperature stress in peach leaves,and related transcription factors that may play a key role in the regulation of cold response pathways were predicted,which provided research direction and basis for the exploration of molecular mechanism of cold response regulation in peach.

Key words: Peach, Leaf, Low temperature, Transcriptome analysis

SHI Meng, JI Xiaohao, YANG Xingwang, WANG Yingying, WANG Haibo, WANG Xiaodi. Transcriptome Analysis of Low Temperature Response in Peach Leaves[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 49-57. doi: 10.7668/hbnxb.20194251.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20194251

http://www.hbnxb.net/EN/Y2023/V38/IS1/49

Figures/Tables 9

References 40

[7]	Wang K, Bai Z Y, Liang Q Y, Liu Q L, Zhang L, Pan Y Z, Liu G L, Jiang B B, Zhang F, Jia Y. Transcriptome analysis of chrysanthemum (Dendranthema grandiflorum) in response to low temperature stress[J]. BMC Genomics, 2018, 19(1): 319. doi:10.1186/s12864-018-4706-x. pmid: 29720105
[8]	张亮, 李疆, 代培红, 罗淑萍, 李鹏, 彭弯弯. 扁桃CBF1转录因子的克隆及原核表达分析[J]. 植物遗传资源学报, 2015, 16(3): 612-617. doi:10.13430/j.cnki.jpgr.2015.03.026.
	Zhang L, Li J, Dai P H, Luo S P, Li P, Peng W W. Cloning and prokaryotic expression of almond CBF1 transcription factor[J]. Journal of Plant Genetic Resources, 2015, 16(3): 612-617. doi: 10.13430/j.cnki.jpgr.2015.03.025
[9]	Mohanta T K, Bashir T, Hashem A, Abd Allah E F. Systems biology approach in plant abiotic stresses[J]. Plant Physiology and Biochemistry, 2017,(121):58-73. doi:10.1016/j.plaphy.2017.10.019.
[10]	张建霞, 李新国, 杨波, 孙中海. Ca²⁺对冷胁迫柑橘离体叶片的相关生理生化指标的影响[J]. 武汉植物学研究, 2005, 23(5): 470-474.
	Zhang J X, Li X G, Yang B, Sun Z. Effect of Ca²⁺ on the correlative physiological and biochemical index of citrus detached leafs under cold stress[J]. Journal of Wuhan Botanical Research, 2005, 23(5): 470-474.
[11]	Cho J H, Lee J H, Park Y K, Choi M N, Kim K N. Calcineurin B-like protein CBL10 directly interacts with TOC34 (translocon of the outer membrane of the chloroplasts) and decreases its GTPase activity in Arabidopsis[J]. Frontiers in Plant Science, 2016, 7: 1911. doi:10.3389/fpls.2016.01911.
[12]	Cheng S H, Willmann M R, Chen H C, Sheen J. Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family[J]. Plant Physiology, 2002, 2(129):469-85. doi:10.1104/pp.005645.
[13]	Shi Y, Ding Y L, Yang S H. Molecular regulation of CBF signaling in cold acclimation[J]. Trends in Plant Science, 2018, 23(7): 623-637. doi:10.1016/j.tplants.2018.04.002. pmid: 29735429
[14]	Zhang H N, Pan X L, Liu S H, Lin W Q, Li Y H, Zhang X M. Genome-wide analysis of AP2/ERF transcription factors in pineapple reveals functional divergence during flowering induction mediated by ethylene and floral organ development[J]. Genomics, 2021, 113(2):474-489. doi:https:10.1016/j.ygeno.2020.10.040. pmid: 33359830
[15]	Ma R F, Xiao Y, Lü Z Y, Tan H X, Chen R B, Li Q, Chen J F, Wang Y, Yin J, Zhang L, Chen W S. AP2/ERF transcription factor, Ii049, positively regulates lignan biosynthesis in Isatis indigotica through activating salicylic acid signaling and lignan/lignin pathway genes[J]. Frontiers in Plant Science, 2017, 8: 1361. doi:10.3389/fpls.2017.01361. URL
[16]	解盼, 刘蔚, 康郁, 华玮, 钱论文, 官春云, 何昕. 甘蓝型油菜CBF基因家族的鉴定和表达分析[J]. 作物学报, 2021, 47(12):2394-2406. doi:10.3724/SP.J.1006.2021.04259.
	Xie P, Liu W, Kang Y, Hua W, Qian L W, Guan C Y, He X. Identification and relative analysis of CBF gene family in Brassica napus L.[J]. Acta Agronomica Sinica, 2021, 47(12):2394-2406.
[17]	Zhang Z, Li X G. Genome-wide identification of AP2/ERF superfamily genes and their expression during fruit ripening of Chinese jujube[J]. Scientific Reports, 2018, 8(1): 15612. doi:10.1038/s41598-018-33744-w. pmid: 30353116
[18]	Kim Y, Choi K, Khan A L, Waqas M, Lee I. Exogenous application of abscisic acid regulates endogenous gibberellins homeostasis and enhances resistance of oriental melon (Cucumis melo var. L.) against low temperature[J]. Scientia Horticulturae, 2016, 207:41-47.doi:10.1016/j.scienta.2016.05.009. URL
[19]	Lenka S K, Muthusamy S K, Chinnusamy V, Bansal K C. Ectopic expression of rice PYL3 enhances cold and drought tolerance in Arabidopsis thaliana[J]. Molecular Biotechnology, 2018, 60(5): 350-361. doi:10.1007/s12033-018-0076-5.
[20]	张颖. 高质量桃线粒体DNA提取技术体系的建立及线粒体基因组测序[D]. 秦皇岛: 河北科技师范学院, 2019.doi: 10.7666/d.D01693611.
	Zhang Y. Establishment of high-quality peach mitochondrial DNA extraction technology system and mitochondrial genome sequencing[D]. Qinhuangdao: Hebei Normal University of Science & Technology, 2019.
[21]	王小丽, 方伟超, 刘云鹤, 赵佩, 毛冬敏, 王新卫. 58份桃种质资源抗寒性评价[J]. 西北林学院学报, 2018, 33(6): 138-144.doi:10.3969/j.issn.1001-7461.2018.06.23.
	Wang X L, Fang W C, Liu Y H, Zhao P, Mao D M, Wang X W. Assessment of the cold resistance of fifty-eight peach accessions[J]. Journal of Northwest Forestry University, 2018, 33(6): 138-144.
[22]	Rec L. Cold hardiness of peaches and nectarines following a test winter[J]. Fruit Varieties Journal, 1982, 4(36):90-98.
[23]	郭学民, 刘建珍, 翟江涛, 肖啸, 吕亚媚, 李丹丹, 裴士美, 张立彬. 16个品种桃叶片解剖结构与树干抗寒性的关系[J]. 林业科学, 2015, 51(8): 33-43.doi: 10.11707/j.1001-7488.20150805.
	Guo X M, Liu J Z, Zhai J T, Xiao X, Lü Y M, Li D D, Pei S M, Zhang L B. Relationship between leaf anatomical structure and trunk cold resistance of 16 peach cultivars[J]. Scientia Silvae Sinicae, 2015, 51(8): 33-43.
[24]	王华, 杨建峰. 植物抗寒基因工程研究进展[J]. 现代农业科技, 2007(23): 117-120, 122. doi: 10.3969/j.issn.1007-5739.2007.23.082
	Wang H, Yang J F. Research progress of plant cold resistance genetic engineering[J]. Xiandai Nongye Keji, 2007(23): 117-120, 122.
[25]	王多佳, 苍晶, 牟永潮, 曾俨, 许平, 于晶. 植物抗寒基因研究进展[J]. 东北农业大学学报, 2009, 40(10)134-138. doi: 10.3969/j.issn.1005-9369.2009.10.029.
	Wang D J, Cang J, Mu Y C, Zeng Y, Xu P, Yu J. Research advance on cold-resistant gene in plants[J]. Journal of Northeast Agricultural University, 2009, 40(10):134-138.
[26]	樊芳菲, 杨暹, 康云艳, 柴喜荣, 蒙林平. 植物DnaJ蛋白的研究进展[J]. 分子植物育种, 2018, 16(6): 2028-2034. doi:10.13271/j.mpb.016.002028.
	Fan F F, Yang X, Kang Y Y, Chai X R, Meng L P. Research progress of plants DnaJ protein[J]. Molecular Plant Breeding, 2018, 16(6): 2028-2034.
[27]	耿伟博. 烟草胚胎发育晚期丰富蛋白(LEA蛋白)基因家族对非生物胁迫反应的鉴定与分析[D]. 泰安: 山东农业大学, 2022.doi:10.27277/d.cnki.gsdnu.2022.000910.
	Geng W B. Identification and analysis of the response of LEA gene family to abiotic stress in late embryonic development of tobacco[D]. Taian: Shandong Agricultural University, 2022.
[28]	Kumar K, Raina S K, Sultan S M. Arabidopsis MAPK signaling pathways and their cross talks in abiotic stress response[J]. Journal of Plant Biochemistry and Biotechnology, 2020, 29(4):700-714.doi:10.1007/s13562-020-00596-3.
[29]	乔永旭. 低温处理过程中水杨酸对红掌叶片生理指标的影响[J]. 东北林业大学学报, 2010, 38(2):11-12, 15. doi: 10.3969/j.issn.1000-5382.2010.02.005.
	Qiao Y X. Effect of salicylic acid on physiological index of Anthurium andraeanum leaves at low temperature[J]. Journal of Northeast Forestry University, 2010, 38(2)11-12, 15.
[30]	陈钰, 郭爱华, 姚延梼. 低温胁迫对杏电解质外渗率的影响[J]. 河南农业科学, 2019, 50(5):85-87. doi: 10.3969/j.issn.1004-3268.2008.02.024.
	Chen Y, Guo A H, Yao Y T. Effects of low temperature stress on electrolyte leakage rate of apricot[J]. Journal of Henan Agricultural Sciences, 2019, 50(5):85-87.
[31]	葛亚英, 田丹青, 郁永明, 潘刚敏, 刘晓静, 夏宜平. 垂吊植物口红花叶片对低温胁迫的生理响应[J]. 浙江大学学报(农业与生命科学版), 2012, 38(5)559-565. doi: 10.3785/j.issn.1008-9209.2011.12.051.
	Ge Y Y, Tian D Q, Yu Y M, Pan G M, Liu X J, Xia Y P. Physiological response of the leaves of the hanging plant Carthamus tinctorius to low temperature stress[J]. Journal of Zhejiang University (Agriculture & Life Sciences), 2012, 38(5):559-565.
[32]	曹群阳, 金亮, 王炜勇, 俞信英, 葛亚英. 低温胁迫对凤剑1号叶片生理的影响[J]. 分子植物育种, 2022:1-8.
	Cao Q Y, Jin L, Wang W Y, Yu X Y, Ge Y Y. Effects of low temperature stress on leaf physiology of Fengjian No. 1[J]. Molecular Plant Breeding, 2022:1-8.
[33]	Zhao C Z, Wang P C, Si T, Hsu C C, Wang L, Zayed O, Yu Z P, Zhu Y F, Dong J, Tao W A, Zhu J K. MAP kinase cascades regulate the cold response by modulating ICE1 protein stability[J]. Developmental Cell, 2017, 43(5): 618-629.e5. doi:10.1016/j.devcel.2017.09.024. pmid: 29056551
[34]	Li H, Ding Y L, Shi Y T, Zhang X Y, Zhang S Q, Gong Z Z, Yang S H. MPK3- and MPK6-mediated ICE1 phosphorylation negatively regulates ICE1 stability and freezing tolerance in Arabidopsis[J]. Developmental Cell, 2017, 43(5): 630-642.e4. doi:10.1016/j.devcel.2017.09.025. URL
[35]	Miura K, Ohta M, Nakazawa M, Ono M, Hasegawa P M. ICE1 Ser403 is necessary for protein stabilization and regulation of cold signaling and tolerance[J]. The Plant Journal, 2011, 67(2): 269-279. doi:10.1111/j.1365-313x.2011.04589.x. pmid: 21447070
[36]	贾瑞玲, 秦倩倩, 张彦萍, 程曦, 梁凯, 侯岁稳. 拟南芥气孔发育的分子遗传机制[J]. 细胞生物学杂志, 2009(6): 817-822.
	Jia R L, Qin Q Q, Zhang Y P, Cheng X, Liang K, Hou S W. Molecular genetic mechanism of stomatal development of Arabidopsis[J]. Chinese Journal of Cell Biology, 2009(6): 817-822.
[37]	肖红菊, 程强. 植物模式识别受体FLS2的研究进展[J]. 南京林业大学学报(自然科学版), 2020, 44(2):220-226.doi:10.3969/j.issn.1000-2006.201904056.
	Xiao H J, Cheng Q. Advances in the study of pattern recognition receptor FLS2 in plants[J]. Journal of Nanjing Forestry University (Natural Science Edition), 2020, 44(2):220-226.
[38]	Chakravarthy S, Tuori R P, D'Ascenzo M D, Fobert P R, Despres C, Martin G B. The tomato transcription factor Pti4 regulates defense-related gene expression via GCC box and non-GCC box cis elements[J]. The Plant Cell, 2003, 15(12): 3033-3050. doi:10.1105/tpc.017574. URL
[39]	Gu Y Q, Wildermuth M C, Chakravarthy S, Loh Y T, Yang C M, He X H, Han Y, Martin G B. Tomato transcription factors pti4, pti5, and pti6 activate defense responses when expressed in Arabidopsis[J]. The Plant Cell, 2002, 14(4): 817-831. doi:10.1105/tpc.000794. URL
[40]	Cyril J, Powell G L, Duncan R R, Baird W V. Changes in membrane polar lipid fatty acids of seashore paspalum in response to low temperature exposure[J]. Crop Science, 2002, 42(6):2031-2037. doi:10.2135/cropsci2002.2031. URL
[1]	Boyer J S. Plant productivity and environment[J]. Science, 1982, 218(4571): 443-448. doi:10.1126/science.218.4571.443. pmid: 17808529
[2]	杨兴旺, 王海波, 王莹莹, 王小龙, 王志强, 刘培培, 刘万春, 王孝娣. 中熟抗寒桃新品种中农甘爽[J]. 园艺学报, 2022, 49(S2):15-16. doi:10.16420/j.issn.0513-353x.2022-0283.
	Yang X W, Wang H B, Wang Y Y, Wang X L, Wang Z Q, Liu P P, Liu W C, Wang X D. A new mid-ripening and cold resistant peach cultivar Zhongnongganshuang[J]. Journal of Horticulture, 2022, 49(S2):15-16.
[3]	Glenn D M, Gusta L, Duman J, Griffith M. Extrinsic ice nucleation in plants: what are the factors and can they be manipulated[J]. Plant Cold Hardiness, 2002:211-221. doi:10.1007/978-1-4615-0711-6_15.
[4]	陈佰鸿, 张彪, 毛娟, 郝燕, 杨瑞, 蔡学海, 齐尚友. 葡萄枝条水分含量变化与抗寒性关系[J]. 植物生理学报, 2014(4): 535-541. doi:10.3969/j.issn.1009-7791.2011.01.021.
	Chen B H, Zhang B, Mao J, Hao Y, Yang R, Cai X H, Qi S Y. Relationship between water content change of grape branches and cold resistance[J]. Plant Physiology Journal, 2014(4): 535-541.
[5]	Mittler R. Oxidative stress, antioxidants and stress tolerance[J]. Trends in Plant Science, 2002, 7(9): 405-410. doi:10.1016/s1360-1385(02)02312-9. pmid: 12234732
[6]	Ma Q B, Dai X Y, Xu Y Y, Guo J, Liu Y J, Chen N, Xiao J, Zhang D J, Xu Z H, Zhang X S, Chong K. Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is mediated by alteration in cell cycle and ectopic expression of stress genes[J]. Plant Physiology, 2009, 150(1): 244-256. doi:10.1104/pp.108.133454. pmid: 19279197

序号 No.	通路 Pathway	ko_ID	差异基因数 DEGs	P-value
1	植物病原体互作	ko04626	12	2.20E-03
2	脂肪酸伸长	ko00062	1	2.08E-02
3	植物激素信号转导	ko04075	5	2.12E-02
4	吞噬体	ko04145	2	4.15E-02
5	不饱和脂肪酸的生物合成	ko01040	1	6.62E-02
6	MAPK信号通路-植物	ko04016	5	1.08E-01
7	昼夜节律-植物	ko04712	2	1.25E-01
8	各种植物次生代谢物的生物合成	ko00999	1	1.60E-01
9	异黄酮的生物合成	ko00943	1	1.64E-01
10	光合作用	ko00195	1	1.69E-01
11	丙氨酸、天冬氨酸和谷氨酸的代谢	ko00250	1	1.73E-01
12	SNARE在水泡运输中的相互作用	ko04130	1	2.03E-01
13	脂肪酸代谢	ko01212	1	2.28E-01
14	色氨酸代谢	ko00380	1	2.40E-01
15	抗坏血酸和藻酸盐代谢	ko00053	1	2.72E-01
16	谷胱甘肽代谢	ko00480	1	2.76E-01
17	RNA降解	ko03018	1	3.73E-01
18	氨基酸的生物合成	ko01230	1	5.05E-01
19	内吞作用	ko04144	1	6.56E-01
20	次生代谢物的生物合成	ko01110	4	7.88E-01
21	代谢途径	ko01100	5	9.98E-01

序号 No.	通路 Pathway	ko_ID	差异基因数 DEGs	P-value
1	植物病原体互作	ko04626	12	2.20E-03
2	脂肪酸伸长	ko00062	1	2.08E-02
3	植物激素信号转导	ko04075	5	2.12E-02
4	吞噬体	ko04145	2	4.15E-02
5	不饱和脂肪酸的生物合成	ko01040	1	6.62E-02
6	MAPK信号通路-植物	ko04016	5	1.08E-01
7	昼夜节律-植物	ko04712	2	1.25E-01
8	各种植物次生代谢物的生物合成	ko00999	1	1.60E-01
9	异黄酮的生物合成	ko00943	1	1.64E-01
10	光合作用	ko00195	1	1.69E-01
11	丙氨酸、天冬氨酸和谷氨酸的代谢	ko00250	1	1.73E-01
12	SNARE在水泡运输中的相互作用	ko04130	1	2.03E-01
13	脂肪酸代谢	ko01212	1	2.28E-01
14	色氨酸代谢	ko00380	1	2.40E-01
15	抗坏血酸和藻酸盐代谢	ko00053	1	2.72E-01
16	谷胱甘肽代谢	ko00480	1	2.76E-01
17	RNA降解	ko03018	1	3.73E-01
18	氨基酸的生物合成	ko01230	1	5.05E-01
19	内吞作用	ko04144	1	6.56E-01
20	次生代谢物的生物合成	ko01110	4	7.88E-01
21	代谢途径	ko01100	5	9.98E-01

Please choose a citation manager

Content to export