Analysis of Differential Genes and Metabolites Pre- and Post-flowering in Aurea helianthus

doi:10.7668/hbnxb.20193972

Abstract

Abstract:

In order to study the changes of genes and metabolites pre- and post-flowering in Aurea helianthus,transcriptome and metabolome techniques were used to detect flower buds and flowers of Aurea helianthus. Through transcriptomic analysis,206 636 Unigenes were identified and 42 618 differentially expressed Unigenes were identified,including 63 differentially expressed transcription factor families and 24 transcription regulatory factor families. GO analysis showed that the differentially expressed genes were mainly enriched in biological processes such as retrograde vesicle-mediated transport. KOG functional annotation showed that the function of differentially expressed genes was mostly general function prediction only,followed by signal transduction mechanism,posttranslational modification,protein turnover,chaperones,and carbohydrate transport and metabolism. KEGG enrichment analysis showed that the differentially expressed genes were mainly enriched in metabolic pathways such as metabolism,plant hormone and signal transduction,starch and sucrose metabolism. A total of 135 metabolites were detected by metabolomics analysis,including lipid,flavonoids,amino acids and derivatives. KEGG enrichment analysis showed that differences metabolites mainly enriched in glycerophospholipid metabolism,glycosylphosphatidylinositol(GPI)-anchor biosynthesis and biosynthesis of secondary metabolites-unclassified,notably,valine,leucine and isoleucine degradation appeared simultaneously in the genes and metabolites KEGG enrichment Top 20 metabolic pathways.

Key words: Aurea helianthus, Flowering, Gene, Metabolite

ZHEN Junbo, SONG Shijia, LIU Linlin, LIU Di, OUYANG Yanfei, CHI Ji'na. Analysis of Differential Genes and Metabolites Pre- and Post-flowering in Aurea helianthus[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 58-66. doi: 10.7668/hbnxb.20193972.

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Figures/Tables 13

References 26

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取样时期 Sampling period	样本名称 Sample name	分组 Group
花蕾Bud	L-1	AhB
花蕾Bud	L-2	AhB
花蕾Bud	L-3	AhB
开花当天Flowering day	H-1	AhF
开花当天Flowering day	H-2	AhF
开花当天Flowering day	H-3	AhF

取样时期 Sampling period	样本名称 Sample name	分组 Group
花蕾Bud	L-1	AhB
花蕾Bud	L-2	AhB
花蕾Bud	L-3	AhB
开花当天Flowering day	H-1	AhF
开花当天Flowering day	H-2	AhF
开花当天Flowering day	H-3	AhF

引物名称 Primer	引物序列(5'—3') Primer sequence(5'—3')	引物用途 Purpose
92208F	CCTTGTCGGGCAAAGTAAACC	荧光定量
92208R	CGGAGGAGCAACTCAGTCAGC	荧光定量
115186F	CGGGAGGTTTCGGGTAATGAT	荧光定量
115186R	CAGGCTTTCCAGAACTGGGTT	荧光定量
92019F	TTGCTCCGAATAGCCTACCG	荧光定量
92019R	CGACAAACACTCCGTCCACC	荧光定量
97829F	TTGGATGATTGGGTTCTTTGC	荧光定量
97829R	CGGGTCCGTTACACTGGTTAT	荧光定量
140405F	AGGAGTATTGGTTCCGTTGGTC	荧光定量
140405R	CATCTTGTCTTGGAGGAGTTGC	荧光定量
87703F	ACGAGTCGGCTAAGATTGTTTC	荧光定量
87703R	CAAGAACTCAACCTGACGCTGT	荧光定量
100232F	TTTCCAGCTTCGTCCGTCAG	荧光定量
100232R	GGCGATGTTGTCACCGTCTT	荧光定量
78465F	GCATGTCTCGGTCGGATGT	荧光定量
78465R	TGGCACGCCTCTTTCAAGT	荧光定量
H2A-1F	TATCTTGCCGCCGAGGTTCT	内参引物
H2A-1R	GAGGAGGTTGTGGATGTTGG	内参引物

引物名称 Primer	引物序列(5'—3') Primer sequence(5'—3')	引物用途 Purpose
92208F	CCTTGTCGGGCAAAGTAAACC	荧光定量
92208R	CGGAGGAGCAACTCAGTCAGC	荧光定量
115186F	CGGGAGGTTTCGGGTAATGAT	荧光定量
115186R	CAGGCTTTCCAGAACTGGGTT	荧光定量
92019F	TTGCTCCGAATAGCCTACCG	荧光定量
92019R	CGACAAACACTCCGTCCACC	荧光定量
97829F	TTGGATGATTGGGTTCTTTGC	荧光定量
97829R	CGGGTCCGTTACACTGGTTAT	荧光定量
140405F	AGGAGTATTGGTTCCGTTGGTC	荧光定量
140405R	CATCTTGTCTTGGAGGAGTTGC	荧光定量
87703F	ACGAGTCGGCTAAGATTGTTTC	荧光定量
87703R	CAAGAACTCAACCTGACGCTGT	荧光定量
100232F	TTTCCAGCTTCGTCCGTCAG	荧光定量
100232R	GGCGATGTTGTCACCGTCTT	荧光定量
78465F	GCATGTCTCGGTCGGATGT	荧光定量
78465R	TGGCACGCCTCTTTCAAGT	荧光定量
H2A-1F	TATCTTGCCGCCGAGGTTCT	内参引物
H2A-1R	GAGGAGGTTGTGGATGTTGG	内参引物

样本 Sample	原始数据 Raw reads	干净序列 Clean reads	错误率/% Error rate	Q30/%	GC含量/% GC content
L-1	73 503 118	71 644 532	0.02	93.56	44.54
L-2	59 489 452	58 093 036	0.02	92.99	44.62
L-3	55 725 072	54 385 274	0.02	93.33	44.46
H-1	59 386 114	58 147 996	0.02	93.38	44.20
H-2	60 959 762	59 618 350	0.02	93.63	44.16
H-3	49 665 928	48 539 808	0.02	93.99	44.26

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