Abstract:

In order to explore wheat leaf rust resistance genes and provide more choices for China's wheat leaf rust resistance genetic breeding gene pool.It selected 75 domestic and foreign wheat materials and 36 known leaf rust resistance gene vector varieties,inoculated 14 physiological races of leaf rust with different virulence at the seedling stage,and identified the severity of the disease of these physiological races with different virulence on wheat materials;simultaneously extract DNA from fresh leaf of all materials,and select specific primers associated with identified leaf rust resistance genes for molecular marker detection on 75 wheat materials.Combining the above two methods,it was speculated that 75 wheat materials contained genes for leaf rust resistance.The results showed that,75 wheat materials contained 13 known leaf rust resistance genes,namely Lr1,Lr2a,Lr2c,Lr10,Lr11,Lr14a,Lr16, Lr18,Lr20,Lr26,Lr34,Lr37 and Lr46,which existed in wheat varieties through single gene or multi gene aggregation.Among them,the Lr1 gene and Lr46 gene account for a relatively large proportion,up to 43% and 56% respectively.These genes exist alone in wheat materials and did not exhibit good resistance to leaf rust;when several or more genes coexisted in wheat materials,they could exhibit much higher leaf rust resistance than a single gene.The results were consistent with previous studies.Molecular markers detected that Lr1,Lr10 and Lr46 genes were contained in S3,a wheat material named large white spring wheat.The identification at the seedling stage in greenhouse showed that the material showed resistance to the 11 physiological races of leaf rust,and it was a relatively good leaf rust resistant wheat.

Key words: Wheat, Leaf rust resistance gene, Gene deduction, Molecular marker detection