Studies on the Changes of Seed Contents in Rosa spinosissima under Low Temperature Sand Storage

doi:10.7668/hbnxb.20193988

Abstract

Abstract:

Rosa spinosissima is valuable germplasm resource for modern rose breeding.R.spinosissima with a wide distribution range has high dormancy characteristics,seed germination is difficult and seed propagation cannot be carried out.It studied the changes of seed contents under different storage methods to explore the factors affecting seed germination.The contents of hormones,organic components and enzymes were respectively determined by ELISA and microassay.The contents were analyzed and compared by one-way analysis of variance and significance test.The results showed that there was no significant difference in the content of seed hormone before and after the accumulation of R.spinosissima.Low temperature sand storage treatment could change the content of organic components and reduce the content of soluble protein,starch and soluble sugar in R.spinosissima seeds.The antioxidant enzyme activities of R.spinosissima seeds were greatly affected by sand storage treatment,and the antioxidant enzyme activities of R.spinosissima seeds were significantly increased after 10 months of low temperature sand storage and sowing.Low temperature treatment was not the main reason to limit the germination of R.spinosissima seeds,but was beneficial to relieve the dormancy of R.spinosissima seeds.It provided some theoretical basis and practical experience for further large-scale production and propagation of wild Rosa resources in Xinjiang.

Key words: Rosa from Xinjiang, Low temperature sand storage, Hormone, Organic composition, Antioxidant enzyme, Seed germination

YANG Yu, LIU Yuchen, ZHOU Meichun, CHENG Bixuan, SONG Jianguo, LUO Le, YU Chao. Studies on the Changes of Seed Contents in Rosa spinosissima under Low Temperature Sand Storage[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 253-260. doi: 10.7668/hbnxb.20193988.

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References 29

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处理 Treatment	种源 Provenance	果实状态 Fruit state	材料处理方法 Material handling method	采集时间/(年-月) Acquisition time
Rs-1	密刺蔷薇	当年成熟果实	未沙藏	2019-09
Rs-2		当年成熟果实	低温沙藏60 d	2019-09
Rs-3		当年成熟果实	低温沙藏60 d并播种10个月后	2020-09
Rs-4		宿存枝条的越冬果实	自然低温	2021-05
Rs-5		当年成熟果实	未沙藏	2021-09
Rs-6		当年成熟果实	低温沙藏20 d	2021-09
Rs-7		当年成熟果实	低温沙藏40 d	2021-09
Rs-8		当年成熟果实	低温沙藏60 d	2021-09
Rp-1	单叶蔷薇	当年成熟果实	未沙藏	2021-10
Rp-2		当年成熟果实	低温沙藏60 d	2021-10
Rb-1	弯刺蔷薇	当年成熟果实	未沙藏	2021-10
Rb-2		当年成熟果实	低温沙藏60 d	2021-10

处理 Treatment	种源 Provenance	果实状态 Fruit state	材料处理方法 Material handling method	采集时间/(年-月) Acquisition time
Rs-1	密刺蔷薇	当年成熟果实	未沙藏	2019-09
Rs-2		当年成熟果实	低温沙藏60 d	2019-09
Rs-3		当年成熟果实	低温沙藏60 d并播种10个月后	2020-09
Rs-4		宿存枝条的越冬果实	自然低温	2021-05
Rs-5		当年成熟果实	未沙藏	2021-09
Rs-6		当年成熟果实	低温沙藏20 d	2021-09
Rs-7		当年成熟果实	低温沙藏40 d	2021-09
Rs-8		当年成熟果实	低温沙藏60 d	2021-09
Rp-1	单叶蔷薇	当年成熟果实	未沙藏	2021-10
Rp-2		当年成熟果实	低温沙藏60 d	2021-10
Rb-1	弯刺蔷薇	当年成熟果实	未沙藏	2021-10
Rb-2		当年成熟果实	低温沙藏60 d	2021-10

处理 Treatment	GA含量 GA content	ABA含量 ABA content	IAA含量 IAA content	ZT含量 ZT content
Rs-5	4.80±0.34a	3 039.80±110.50a	793.83±63.02a	3.29±0.27a
Rs-8	4.77±0.41a	3 131.20±95.03a	714.00±12.01a	3.44±0.21a
Rp-1	4.84±0.31a	3 068.90±68.23a	727.13±6.35a	3.47±0.10a
Rp-2	4.82±0.20a	2 783.80±64.62b	762.53±85.52a	3.47±0.20a
Rb-1	4.77±0.23a	2 825.93±44.27b	752.40±61.32a	3.45±0.18a
Rb-2	4.86±0.24a	2 822.53±67.80b	766.97±64.55a	3.40±0.13a

处理 Treatment	GA含量 GA content	ABA含量 ABA content	IAA含量 IAA content	ZT含量 ZT content
Rs-5	4.80±0.34a	3 039.80±110.50a	793.83±63.02a	3.29±0.27a
Rs-8	4.77±0.41a	3 131.20±95.03a	714.00±12.01a	3.44±0.21a
Rp-1	4.84±0.31a	3 068.90±68.23a	727.13±6.35a	3.47±0.10a
Rp-2	4.82±0.20a	2 783.80±64.62b	762.53±85.52a	3.47±0.20a
Rb-1	4.77±0.23a	2 825.93±44.27b	752.40±61.32a	3.45±0.18a
Rb-2	4.86±0.24a	2 822.53±67.80b	766.97±64.55a	3.40±0.13a

处理 Treatment	SOD (U/mg)	CAT (nmol/(min·mg))	POD (U/mg)	PAL (U/mg)	APX (nmol/(min·mg))	MDA (nmol/g)
Rs-5	8.05±0.73b	1.11±0.08cd	2.39±0.13a	1.62±0.13d	19.19±1.46d	35.70±1.31e
Rs-8	10.55±0.60b	5.72±0.08b	2.22±0.38a	2.95±0.14b	150.95±3.30a	23.73±2.12d
Rp-1	0.48±0.01c	0.75±0.01d	0.65±0.22c	2.56±0.16c	1.96±0.15e	60.02±3.36b
Rp-2	0.49±0.29c	2.85±0.01cd	0.66±0.10c	4.09±0.06a	35.46±1.26b	105.30±6.21a
Rb-1	18.88±0.64a	3.62±0.31bc	1.62±0.16b	1.36±0.18d	26.95±3.49c	19.52±0.63d
Rb-2	21.37±2.97a	18.73±3.10a	2.11±0.15a	1.53±0.27d	18.32±1.79d	22.02±0.79d

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