Effects of Cd on Physiological and Biochemical Processes of Wheat CM28 under Glycine Spray

doi:10.7668/hbnxb.20194912

Abstract

Abstract:

Cd-sensitive wheat CM28 was used as the research object to investigate the effects of spraying exogenous glycine on its physiological and biochemical processes under different concentrations of cadmium stress.The plant height and root length,above-ground transport coefficient and enrichment coefficient of cadmium,chlorophyll content and malondialdehyde(MDA)content,and the enzyme activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)of CM28 under different treatments were determined.The expression levels of PEPC genes in roots and leaves under different treatments were analyzed by qRT-PCR.The results showed that under 30 μmol/L Cd stress,the spraying of exogenous glycine led to a decrease in the above-ground dry and fresh weights,a decrease in the total-chlorophyll content,and an increase in the MDA content of CM28,indicating that plants were affected by more reactive oxygen species.Under 50 μmol/L cadmium stress,glycine could effectively alleviate the negative effects of cadmium on CM28,resulting in an increase in the total-chlorophyll content,an increase in the above-ground dry and fresh weights,and a decrease in MDA content,suggesting that the plant was affected by reactive oxygen species to a greater extent.The antioxidant enzyme activity of CM28 increased with the increase of cadmium concentration,and the spraying of exogenous glycine could enhance the activity of antioxidant enzymes and alleviate the effect of cadmium on the whole.The expression of PEPC genes in roots increased overall with the rise of cadmium concentration,indicating that PEPC played an important role in the process of plant root resistance to stress,and the spraying of exogenous glycine can also effectively alleviate the cadmium stress on CM28 and reduce the expression of PEPC genes on the whole.In summary,cadmium stress negatively affects the physiological and biochemical processes of CM28,and the spraying exogenous glycine can alleviate the effects.

Key words: Triticum aestivum L., Cadmium stress, Reactive oxygen species, Antioxidant enzyme system, PEPC gene

DENG Haoyu, WU Yichao, FU Teng, YANG Zaijun, WU Rina. Effects of Cd on Physiological and Biochemical Processes of Wheat CM28 under Glycine Spray[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 63-71. doi: 10.7668/hbnxb.20194912.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20194912

http://www.hbnxb.net/EN/Y2024/V39/IS1/63

Figures/Tables 9

References 21

[1]	茹淑华, 徐万强, 赵欧亚, 孙世友, 刘蕾, 肖广敏, 侯利敏. 河北省典型区域土壤-农产品重金属积累特征及风险评价[J]. 华北农学报, 2023, 38(S1):339-347.doi:10.7668/hbnxb.20193630.
	Ru S H, Xu W Q, Zhao O Y, Sun S Y, Liu L, Xiao G M, Hou L M. Accumulation characteristics and risk assessment of heavy metals in soil-agricultural products in typical regions of Hebei Province[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1):339-347. doi: 10.7668/hbnxb.20193630
[2]	王效瑾. 小麦镉吸收和转运的品种间差异研究[D]. 郑州: 河南农业大学, 2020.doi:10.27117/d.cnki.ghenu.2020.000295.
	Wang X J. Study on the differences varieties of wheat cadmium uptake and translocation[D]. Zhengzhou: Henan Agricultural University, 2020.
[3]	张芬琴. 镉胁迫对二种不同耐性豆科植物生长与活性氧代谢的影响及水杨酸对镉毒害的缓解效应[D]. 南京: 南京农业大学, 2009.doi:10.7666/d.Y1764437.
	Zhang F Q. Effect of cadmium on growth and oxidative metabolism as well as role of salicylic acid in alleviating cadmium toxicity in two leguminous species with different tolerance[D]. Nanjing: Nanjing Agricultural University, 2009.
[4]	冯亚娟, 李廷轩, 蒲勇, 张锡洲. 不同镉积累类型小麦各器官镉积累分布规律及机理分析[J]. 作物学报, 2022, 48(7):1761-1770.doi:10.3724/SP.J.1006.2022.11046.
	Feng Y J, Li T X, Pu Y, Zhang X Z. Characteristics of cadmium accumulation and distribution in different organs of wheat with different cadmium-accumulating type[J]. Acta Agronomica Sinica, 2022, 48(7):1761-1770.
[5]	Rizwan M, Ali S, Rehman M Z U, Maqbool A. A critical review on the effects of zinc at toxic levels of cadmium in plants[J]. Environmental Science and Pollution Research, 2019, 26(7):6279-6289.doi:10.1007/s11356-019-04174-6.
[6]	孙弘, 刘合刚, 崔谨谨, 刘小京, 杨丽琳, 徐进. 外源甘氨酸对镉胁迫下苜蓿幼苗生长和氧化损伤的影响[J]. 中国生态农业学报, 2010, 18(5):1022-1025.doi:10.3724/SP.J.1011.2010.01022.
	Sun H, Liu H G, Cui J J, Liu X J, Yang L L, Xu J. Effect of exogenous Gly on the growth and oxidative damage of alfalfa seedling under Cd stress[J]. Chinese Journal of Eco-Agriculture, 2010, 18(5):1022-1025.
[7]	叶志谦, 黄腾波, 黄健子. 植物PEPC在非生物胁迫响应中的作用[J]. 植物生理学报, 2023, 59(2):267-280.doi:10.13592/j.cnki.ppj.300105.
	Ye Z Q, Huang T B, Huang J Z. The role of plant PEPC in response to abiotic stress[J]. Plant Physiology Journal, 2023, 59(2):267-280.
[8]	杨永志, 高伟, 高翔, 钟龙, 李菊, 王朝弼, 王汀忠, 张珂, 阮云泽. 外源甘氨酸促进火龙果根系生长和养分吸收[J]. 热带作物学报, 2022, 43(7):1440-1449.doi:10.3969/j.issn.1000-2561.2022.07.015.
	Yang Y Z, Gao W, Gao X, Zhong L, Li J, Wang C B, Wang T Z, Zhang K, Ruan Y Z. Exogenous glycine promotes pitaya(Hylocereus polyrhizus)root growth and nutrient absorption[J]. Chinese Journal of Tropical Crops, 2022, 43(7):1440-1449.
[9]	牛娟娟, 方淑梅, 王庆燕, 梁喜龙. 不同浓度甘氨酸拌土对水稻秧苗生长特性的影响[J]. 中国稻米, 2024, 30(4):47-52. doi: 10.3969/j.issn.1006-8082.2024.04.009
	Niu J J, Fang S M, Wang Q Y, Liang X L. Effects of different concentrations of glycine mixed in soil on growth characteristics of rice seedlings[J]. China Rice, 2024, 30(4):47-52. doi: 10.3969/j.issn.1006-8082.2024.04.009
[10]	葛体达, 宋世威, 姜武, 唐东梅, 黄丹枫. 不同甘氨酸浓度对无菌水培番茄幼苗生长和氮代谢的影响[J]. 生态学报, 2009, 29(4):1994—2002.doi:10.3321/j.issn:1000-0933.2009.04.045.
	Ge T D, Song S W, Jiang W, Tang D M, Huang D F. Influence of glycine-N concentration on the growth and nitrogen metabolism of tomato seedlings under sterile hydroponics cultivation[J]. Acta Ecologica Sinica, 2009, 29(4):1994-2002.
[11]	纪思萌, 陈真勇, 彭正松, 廖明莉, 欧阳钟鸣, 杨在君. 小麦三雌蕊突变体与四川主要小麦品种籽粒的微量元素含量分析[J]. 西华师范大学学报(自然科学版), 2019, 40(2):119—123,196.doi:10.16246/j.issn.1673-5072.2019.02.003.
	Ji S M, Chen Z Y, Peng Z S, Liao M L, Ouyang Z M, Yang Z J. An analysis of micronutrients content in wheat grain of three pistils mutants and cultivars in Sichuan Province[J]. Journal of China West Normal University(Natural Sciences), 2019, 40(2):119-123,196.
[12]	梁娜. 植物吸收污染土壤中镉的规律[J]. 山西水土保持科技, 2023(1):40-42.doi:10.3969/j.issn.1008-0120.2023.01.014.
	Liang N. Study on the uptake cadmium in the contaminated soil by plants[J]. Soil and Water Conservation Science and Technology in Shanxi, 2023(1):40-42.
[13]	李丽菁, 张智韦, 薛云, 张嘉航, 韩烈保, 许立新. 低温胁迫对日本结缕草叶绿素代谢的影响[J]. 北京林业大学学报, 2022, 44(2):91-99.doi:10.12171/j.1000-1522.20200400.
	Li L J, Zhang Z W, Xue Y, Zhang J H, Han L B, Xu L X. Effects of low temperature stress on chlorophyll metabolism of Zoysia japonica[J]. Journal of Beijing Forestry University, 2022, 44(2):91-99.
[14]	易镇邪, 屠乃美, 敖和军, 陈平平. 土壤酸化背景下镉污染稻田水稻镉吸收阻控研究方向探讨[J]. 湖南生态科学学报, 2020, 7(1):50-57.doi:10.3969/j.issn.2095-7300.2020.01.008.
	Yi Z X, Tu N M, Ao H J, Chen P P. Discussion on research directions on controlling of rice Cd absorption in Cd polluted paddy field under soil acidification[J]. Journal of Hunan Ecological Science, 2020, 7(1):50-57.
[15]	李梓萌, 李肖乾, 张文慧, 纪艺凝, 孙思宇, 王素静, 安启睿, 李鹏洋, 郑娜. 重金属复合污染对生物影响的研究进展[J]. 环境化学, 2021, 40(11):3331-3343.doi:10.7524/j.issn.0254-6108.2021033107.
	Li Z M, Li X Q, Zhang W H, Ji Y N, Sun S Y, Wang S J, An Q R, Li P Y, Zheng N. Research progress on the effects of heavy metal compound pollution on organisms[J]. Environmental Chemistry, 2021, 40(11):3331-3343.
[16]	王小丽. 小白菜对甘氨酸态氮的吸收代谢及生理响应[D]. 上海: 上海交通大学, 2014.
	Wang X L. Uptake,metabolism and physiological responses of pakchoi under glycine-nitrogen nutrition conditions[D]. Shanghai: Shanghai Jiao Tong University, 2014.
[17]	张俊岭, 李柏森, 杨丽云, 李娅琼. 重金属镉对药用大黄叶绿素含量及细胞超微结构的影响[J]. 中药材, 2023, 46(1):32-35.doi:10.13863/j.issn1001-4454.2023.01.006.
	Zhang J L, Li B S, Yang L Y, Li Y Q. Effects of cadmium on chlorophyll content and cell ultrastructure of medicinal rhubarb[J]. Journal of Chinese Medicinal Materials, 2023, 46(1):32-35.
[18]	吴芳芳, 郑有飞, 吴荣军, 王锦旗. 近地层臭氧对小麦抗氧化酶活性变化动态的影响[J]. 生态学报, 2011, 31(14):4019-4026.
	Wu F F, Zheng Y F, Wu R J, Wang J Q. Concentration of O₃ at the atmospheric surface affects the changes characters of antioxidant enzyme activities in Triticum aestivum[J]. Acta Ecologica Sinica, 2011, 31(14):4019-4026.
[19]	Cho U H, Park J O. Mercury-induced oxidative stress in tomato seedlings[J]. Plant Science, 2000, 156(1):1-9.doi:10.1016/s0168-9452(00)00227-2. pmid: 10908800
[20]	Shah K, Kumar R G, Verma S, Dubey R S. Effect of cadmium on lipid peroxidation,superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings[J]. Plant Science, 2001, 161(6):1135-1144.doi:10.1016/s0168-9452(01)00517-9.
[21]	冯福生, 马力耕. 低温对玉米叶片PEP羧化酶及其调节特性的影响[J]. 植物生理学报, 1992, 18(1):45-49.
	Feng F S, Ma L G. The effect of low temperature on the activity of phosphoenolpyruvate carboxylase of maize leaves and its regulatory properties[J]. Plant Physiology Journal, 1992, 18(1):45-49.

引物名称 Primer name	上游引物序列(5'-3') Forward primer sequence(5'-3')	下游引物序列(5'-3') Reverse primer sequence(5'-3')
Tappc3	CAACATTTGGACTATCCCTTG	TTGGCGTTTCTCCTCTGAC
Tappc1b	TCCCCCAAGCCCGAGT	AGGTGTCGCCGATCTGAAGTA
Tappc4	CCAACTACGCCGAGACACAGA	ACAGCCGCTGCCTCAGGTA
Tappc-b	ACCAAAACAGGACCAGAAAAC	GGAAGATCAGCACCAGAAGG
Tappc2	TGTCCGAGGACGACAAGCTC	TCTTGAACCATCTCCCTGAGATC
Tappc1a	CGCGAATTCGTGCAGGA	ATGGAGTCAGCGGGGGA

引物名称 Primer name	上游引物序列(5'-3') Forward primer sequence(5'-3')	下游引物序列(5'-3') Reverse primer sequence(5'-3')
Tappc3	CAACATTTGGACTATCCCTTG	TTGGCGTTTCTCCTCTGAC
Tappc1b	TCCCCCAAGCCCGAGT	AGGTGTCGCCGATCTGAAGTA
Tappc4	CCAACTACGCCGAGACACAGA	ACAGCCGCTGCCTCAGGTA
Tappc-b	ACCAAAACAGGACCAGAAAAC	GGAAGATCAGCACCAGAAGG
Tappc2	TGTCCGAGGACGACAAGCTC	TCTTGAACCATCTCCCTGAGATC
Tappc1a	CGCGAATTCGTGCAGGA	ATGGAGTCAGCGGGGGA

处理 Treatment	根鲜质量 Roots fresh weight	根干质量 Roost dry weight	地上部鲜质量 Aboveground fresh weight	地上部干质量 Aboveground dry weight
CK	14.56±0.003a	0.57±0.015b	33.22±0.785a	3.48±0.002a
Cd30	12.40±0.002b	0.51±0.001c	32.61±1.390a	2.60±0.002b
Cd50	6.61±0.001e	0.35±0.001d	14.88±0.125b	1.77±0.010e
Cd30+Gly	10.22±0.001c	0.60±0.001b	15.42±0.415b	2.04±0.002c
Cd50+Gly	7.61±0.002d	0.29±0.003e	13.69±1.685b	1.94±0.002d

处理 Treatment	根鲜质量 Roots fresh weight	根干质量 Roost dry weight	地上部鲜质量 Aboveground fresh weight	地上部干质量 Aboveground dry weight
CK	14.56±0.003a	0.57±0.015b	33.22±0.785a	3.48±0.002a
Cd30	12.40±0.002b	0.51±0.001c	32.61±1.390a	2.60±0.002b
Cd50	6.61±0.001e	0.35±0.001d	14.88±0.125b	1.77±0.010e
Cd30+Gly	10.22±0.001c	0.60±0.001b	15.42±0.415b	2.04±0.002c
Cd50+Gly	7.61±0.002d	0.29±0.003e	13.69±1.685b	1.94±0.002d

处理 Treatment	叶片镉含量 Cadmium content in leaves	根镉含量 Cadmium content in roots
CK	3.9±0.071e	51.0±1.587e
Cd30	96.1±2.458d	1 050.9±10.026a
Cd50	158.5±2.611b	601.6±0.000d
Cd30+Gly	106.1±2.959c	643.5±0.000c
Cd50+Gly	170.0±2.907a	689.4±0.000b

Please choose a citation manager

Content to export