Expression Pattern of the OsRboh Family Genes in Rice under Heat Stress of Different Growth Stages

doi:10.7668/hbnxb.20193902

Abstract

Abstract:

In order to explore the gene expression regulation mechanism in the formation of reactive oxygen species (ROS) induced by heat stress,heat stress treatments were set at seedling,heading and filling stage to investigate the dynamic change of ROS accumulation in rice,respectively.And the Quantitative Real-time PCR (qRT-PCR) was used to analyze the expression pattern of nine respiratory burst oxidase homologue (Rboh) encoding genes (OsRboh1-OsRboh9) in rice under different growth stages.Results showed that the ROS accumulation in rice leaves and grains significantly increased with the extension of heat stress.The ROS content increased slowly after 7 days of heat stress at seedling stage, while increased continuously after heat stress during the heading stage and early filling stage (1-10 d) in rice grains. Expression levels of the nine OsRboh family genes continuously increased with the extension of heat stress at seedling and heading stages,and OsRboh7 and OsRboh5 showed higher expression levels under heat stress.The expression levels of OsRboh1,OsRboh5 and OsRboh9 were continuously increased,while other genes showed a change tendency of increasing initially and then decreasing under heat stress at filling stage.The expression levels of OsRboh7 and OsRboh5 were all reached to a high level at seedling,heading and filling stage under heat stress.Furthermore,higher expression levels of OsRboh7 and OsRboh5 were showed in various tissues and organs of rice such as seedling leaves,flag leaf,floret,lemma,palea,stamen,pistil and grain.The higher induced range of gene expression levels in OsRboh7 and OsRboh5 by heat stress was shown in seedling leaves,floret,stamen,pistil and grain.Taken together,OsRboh7 and OsRboh5 were mostly remarkably responsive to heat stress at different growth stages among the OsRboh family genes in rice,which indicated that OsRboh7 and OsRboh5 played a vital role in the regulation of ROS formation pathway in rice under heat stress condition.

Key words: Rice, Reactive oxygen species, Respiratory burst oxidase homologue, Expression pattern, qRT-PCR

LIU Xiaolong, LIAO Jingpeng, ZHONG Xin, DUAN Ximiao, HU Yongxuan, LIU Jiacheng, LIU Zekai, YANG Hongtao. Expression Pattern of the OsRboh Family Genes in Rice under Heat Stress of Different Growth Stages[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(5): 1-9. doi: 10.7668/hbnxb.20193902.

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Figures/Tables 7

Tab.1 Genes and related primers for qRT-PCR

基因名称 Gene name	引物序列(5'-3') Primer sequence(5'-3')
OsRboh1(Os01g0360200)	F:AAGGGAATAACGGACGAAA	R:CCTCTGAACCACTCAAACG
OsRboh2(Os01g0734200)	F:CACAACTACCTAACAAGCGTC	R:TCCTCACCTTGCTATCTCC
OsRboh3(Os01g0835500)	F:CTTTTGCTTATTGGTCTTGG	R:TTGTTTCGTGAGTGTAGGG
OsRboh4(Os05g0465800)	F:CAAGCGAGGTGTTTGTGGCA	R:GGCTGTCACCATACCACGGA
OsRboh5(Os05g0528000)	F:TTACTGCTGGTTGGATTAGGA	R:CATAGTAATGAGTGCTGACCGA
OsRboh6(Os08g0453700)	F:GAACGCTTGGCACGAAATAG	R:GAACGCTTGGCACGAAATAG
OsRboh7(Os09g0438000)	F:GTCAAATGCTTATGCTGTCA	R:TGTCCAGTCTCCGTTTGTT
OsRboh8(Os11g0537400)	F:CCCAGCAACCTCGGCTACAT	R:ACGCAGACGCAGTAGCCCAT
OsRboh9(Os12g0541300)	F:CCGTAAGGATTGAGAAGGT	R:GGGTCGTCGTAGATGTGGT

Fig.1 Changes of

O 2 -

and H₂O₂ content in rice under heat stress of different growth stages Different small letters indicate the significant difference at P<0.05 level between different treatments.The same as Fig.2-6.

Fig.1 Changes of $O 2 -$ and H₂O₂ content in rice under heat stress of different growth stages Different small letters indicate the significant difference at P<0.05 level between different treatments.The same as Fig.2-6.

Fig.2 Expression pattern of the OsRboh family genes in rice under heat stress at seedling stage

Fig.3 Expression pattern of the OsRboh family genes in rice under heat stress at heading stage

Fig.4 Expression pattern of the OsRboh family genes in rice under heat stress at filling stage

Fig.5 Differences of expression levels of the OsRboh family genes in rice under different heat stress point

Fig.6 Expression pattern of OsRboh5 and OsRboh7 in different tissue organs in rice

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