Study on Adaptability of Different Hybrid Types of Brassica napus in Heavy Metal Contaminated Soil

doi:10.7668/hbnxb.20193784

Abstract

Abstract:

Heavy metal pollution in soil has seriously affected the development of agriculture and the safety of agricultural products in China.Phytoremediation is a widely concerned soil remediation method,and rape is an ideal remediation plant.In order to screen rape breeding materials suitable for using as remediation plants.The pot experiment was carried out.With 100% nutrient soil environment as a control,three polluted soil environments(Ⅱ(25% contaminated soil,75% nutrient soil), Ⅲ(50% contaminated soil,50% nutrient soil), Ⅳ(75% contaminated soil,25% nutrient soil)with different proportions of contaminated soil were set up.The physiological characteristics and gene expression differences of three different hybrid types of Brassica napus,159-6(Self crossing species),Fengyou 520(Hybrid),and 159-6×Fengyou 520(Triple cross),were studied under different ratios of heavy metal polluted soil environments.The results showed that under different ratios of heavy metal contaminated soil,159-6×Fengyou 520 had the highest fresh and dry quality and was higher than control group I.Under 25% polluted soil environment,159-6 had the highest chlorophyll content,while the rest had 159-6 chlorophyll content×Fengyou 520 had the highest chlorophyll content.Except for Fengyou 520 with the highest soluble protein content in a 50% polluted soil environment,the rest were 159-6×Fengyou 520 had the highest soluble protein content.Under 50% and 75% polluted soil environment,159-6×Fengyou 520 had the highest SOD activity,with MDA content lower than 159-6 and Fengyou 520;four genes related to heavy metal resistance(BnaA08g04000D,BnaA09g24330D,BnNRAMP1,and BnPri-miR167a)were identified in 159-6×The expression levels in the leaves of Fengyou 520 were higher than those of 159-6 and Fengyou 520;Bna0280620 and Bna049040 genes in 159-6 under 75% polluted soil environment×The expression level in the leaves of Fengyou 520 was also higher than that of Fengyou 520 and 159-6.159-6,Fengyou 520 and 159-6×Fengyou 520 could grow and develop normally in polluted soil environments with different ratios;Sanjiao rapeseed 159-6×Fengyou 520 performs better in environments containing 50% and 75% polluted soil.The study was carried out from the genotype and biological characteristics of breeding materials,and the differences between conventional materials and hybrid materials in heavy metal resistance and between different hybrid types were analyzed.It was found that hybrids were better than conventional materials,while three hybrids were better than hybrids.

Key words: Brassica napus L., Heavy metals, Antioxidant enzymes, Phytoremediation

PENG Duozi, HUANG Haoyuan, FAN Zhanhuang, DAI Yue, YUAN Miaoxin, ZHANG Zhenqian. Study on Adaptability of Different Hybrid Types of Brassica napus in Heavy Metal Contaminated Soil[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(4): 181-189. doi: 10.7668/hbnxb.20193784.

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References 35

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基因名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
BnActin	F:GGTTGGGATGGACCAGAAGG
	R:TCAGGAGCAATACGGAGC
Bna0280620	F:CAGCCGATTATGTTAGACCC
	R:GTCTCCAGCCTCCACATCGT
Bna0449040	F:TGGCTATGTTAGGCTCTTT
	R:AGCAGCACCAACTATGAGA
BnaC08g46180D	F:GGGGACCAAGTAAATACCA
	R:AGCAGGAACCCAAACTACC
BnaA08g04000D	F:TTGAGTCTTCGGTCGCACTT
	R:TGCCGCTAACAAACCAATTCC
BnaA09g24330D	F:AGCGACCCAAGGATTCCAAA
	R:AGTTTGCATGTTACATCACCGT
BnPri-miR167a	F:GGTGAAGTGAACGGTGTA
	R:GTGAAATTTGAGATGGGA
BnNRAMP1	F:TTTGCTATGGGTTGTTGC
	R:CTTCTCCTGGGTCTGGTT

基因名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
BnActin	F:GGTTGGGATGGACCAGAAGG
	R:TCAGGAGCAATACGGAGC
Bna0280620	F:CAGCCGATTATGTTAGACCC
	R:GTCTCCAGCCTCCACATCGT
Bna0449040	F:TGGCTATGTTAGGCTCTTT
	R:AGCAGCACCAACTATGAGA
BnaC08g46180D	F:GGGGACCAAGTAAATACCA
	R:AGCAGGAACCCAAACTACC
BnaA08g04000D	F:TTGAGTCTTCGGTCGCACTT
	R:TGCCGCTAACAAACCAATTCC
BnaA09g24330D	F:AGCGACCCAAGGATTCCAAA
	R:AGTTTGCATGTTACATCACCGT
BnPri-miR167a	F:GGTGAAGTGAACGGTGTA
	R:GTGAAATTTGAGATGGGA
BnNRAMP1	F:TTTGCTATGGGTTGTTGC
	R:CTTCTCCTGGGTCTGGTT

基因 Gene	不同类型油菜 Different types of rapeseed	鲜质量 Fresh weight	干质量 Dry weight	叶绿素含量 Chlorophyll content	可溶性糖含量 Soluble sugar content	可溶性蛋白含量 Soluble protein content
Bna0280620	沣油520	0.603	-0.061	-0.023	-0.032	-0.147
	159-6	-0.736^*	-0.462	0.147	0.528	-0.789^*
	159-6×沣油520	-0.091	-0.292	-0.289	-0.333	0.812^**
Bna049040	沣油520	-0.587	0.165	0.212	0.199	0.280
	159-6	-0.542	-0.224	0.418	0.435	-0.607
	159-6×沣油520	-0.389	-0.481	-0.496	0.716^*	-0.094
BnaC08g46180D	沣油520	-0.738^*	-0.486	-0.891^**	-0.759^*	-0.834^**
	159-6	-0.492	-0.706^*	-0.974^**	0.364	-0.469
	159-6×沣油520	0.846^**	0.753^*	0.788^*	0.491	-0.990^**

基因 Gene	不同类型油菜 Different types of rapeseed	鲜质量 Fresh weight	干质量 Dry weight	叶绿素含量 Chlorophyll content	可溶性糖含量 Soluble sugar content	可溶性蛋白含量 Soluble protein content
Bna0280620	沣油520	0.603	-0.061	-0.023	-0.032	-0.147
	159-6	-0.736^*	-0.462	0.147	0.528	-0.789^*
	159-6×沣油520	-0.091	-0.292	-0.289	-0.333	0.812^**
Bna049040	沣油520	-0.587	0.165	0.212	0.199	0.280
	159-6	-0.542	-0.224	0.418	0.435	-0.607
	159-6×沣油520	-0.389	-0.481	-0.496	0.716^*	-0.094
BnaC08g46180D	沣油520	-0.738^*	-0.486	-0.891^**	-0.759^*	-0.834^**
	159-6	-0.492	-0.706^*	-0.974^**	0.364	-0.469
	159-6×沣油520	0.846^**	0.753^*	0.788^*	0.491	-0.990^**

基因 Gene	不同类型油菜 Different types of rapeseed	SOD活性 SOD activity	POD活性 POD activity	CAT活性 CAT activity	MDA含量 MDA content
BnaA08g04000D	沣油520	-0.944^**	0.100	-0.773^*	-0.747^*
	159-6	-0.289	-0.132	0.415	-0.502
	159-6×沣油520	-0.333	0.745^*	-0.861^**	0.746^*
BnNRAMP1	沣油520	-0.951^**	0.207	-0.860^**	0.886^**
	159-6	-0.258	-0.203	0.392	-0.482
	159-6×沣油520	-0.609	0.891^**	-0.827^**	0.805^**
BnPri-miR167a	沣油520	-0.903^**	0.197	-0.931^**	0.909^**
	159-6	0.077	-0.464	0.715^*	-0.779^*
	159-6×沣油520	-0.643	0.912^**	-0.777^*	0.783^*
BnaA09g24330D	沣油520	-0.800^**	-0.083	-0.356	0.370
	159-6	0.236	-0.587	0.779^*	-0.818^**
	159-6×沣油520	-0.589	0.905^**	-0.844^*	0.800^**

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