Abstract:

The purpose of this paper was to clone the CDS region of StAR gene,perform bioinformatics and detect StAR gene expression level among different tissues using RT-qPCR in order to revel its function.The results showed that the CDS region of Hezuo pig StAR gene was 858 bp in length,encoding 285 amino acids.Compared to the reference sequence of pig,the A at the 572nd point mutation to G(leading to lysine mutation to arginine at the 191st point)was missense mutation and insertion of a base C at the 791st point(causing 20 amino acids mutation)was frame shift mutation,respectively.Molecular mass was about 90.95 ku and theoretical isoelectric point was 8.87;extinction coefficient was 33 835 and unstable coefficient was 41.49.Additionally,it contained one N-sugar-based site and secondary structure was mainly composed of α spiral and irregular curling.The secondary structure was similar to the tertiary structure.Lastly,the similarity of StAR nucleic acid sequences among Hezuo pig and Sus scrofa, Ovis aries, Bos taurus was 99.77%, 90.45% and 91.78%,respectively.The phylogenetic tree result showed that Hezuo pig was closed to pig.StAR gene expressed in these tissues,such as spleen,kidney,lung.Among these tissues,testis have higher expression,indicating that it may be related to the development testis in Hezuo pig.

Key words: Hezuo pig, StAR gene, Bioinformatics, Tissue expression