Abstract:

The aim was to clone the IGF-1 gene of Gannan yak,conduct bioinformatics analysis and detect its expression pattern in different tissues,so as to provide a reference for studying its function.Taking the cDNA of testicular tissue of Gannan yaks as the template,the CDS region of the IGF-1 gene was amplified by PCR,and then bioinformatics analysis was carried out.Subsequently,Quantitative Real-time PCR(RT-qPCR)was used to detect its expression patterns in heart,liver,lung,kidney,spleen,muscle,testicular and adipose tissues.The results showed that the CDS region of the IGF-1 gene was Gannan yak was 567 bp in total length and encoded 188 amino acids.The molecular formula of IGF-1 protein was C₉₀₇H₁₄₆₄N₂₇₂O₂₆₅S₁₇,with an isoelectric point of 9.72 and an instability index of 69.91,which belonged to unstable proteins.The IGF-1 protein was a hydrophilic secreted protein containing signal peptides.It was mainly located outside the cell and contained 10 O-glycosylation and 19 phosphorylation sites.The secondary structure was mainly composed of random coil and α-helix.It interacted with proteins such as IGF1R,IGFBP3,INSR and IGFBP1.The results of RT-qPCR showed that the expression level of IGF-1 gene in the liver of Gannan yak was extremely significantly higher than that in other tissues,and the expression levels in the spleen and fat were extremely significantly higher than those in the lungs,testicles and muscles.

Key words: Gannan yak, IGF-1 gene, Cloning, Bioinformatics, Tissue expression

CLC Number: 

• S823

• Q78基因工程(遗传工程)