Whole Transcriptome Sequencing was Used to Explore Genes and miRNAs Related to Spermatogenesis in Blcattle Black Cattle

doi:10.7668/hbnxb.20195752

Abstract

Abstract:

This study aimed to explore the molecular mechanism of spermatogenesis and address the decline of semen quality in male animals by analyzing the transcriptome data of the testicles of large and small Blcattle black cattle and mining the key genes and microRNAs(miRNAs)related to spermatogenesis.Six 12-month-old black cattle testicular tissues were collected from Blcattle black cattle as experimental animals,and the testicles were divided into large and small testis groups according to testicular weight,length diameter and short diameter.The whole transcriptome library was constructed,and the differential expression genes(DEGs)were screened with |log₂(Fold Change)|≥1 and FDR<1 as the threshold,and the differential expressed microRNAs(DEMs)were screened with |log₂(Fold Change)|≥1 and P≤0.05 as the threshold.GO and KEGG enrichment analysis screened the biological items and pathways that affect spermatogenesis,weighted correlation network analysis (WGCNA)screened the gene groups associated with testicular traits,integrated protein protein interaction(PPI),and finally screened out candidate genes related to spermatogenesis and predicted to target DEMs.The results showed that a total of 3 935 DEGs(2 049 up-regulated,1 886 down-regulated)were screened in the transcriptome library,and the differentially annotated genes of GO function were significantly enriched in signal transduction and MAPK cascade.KEGG showed that FGFR3,NFKB1,MAP3K5 and TAB1 were significantly enriched in the MAPK cascade and sphingolipid signaling pathway,and 6 762 and 295 genes were strongly correlated with testicular size,respectively.The integration of PPI protein-protein interaction identified 20 differential genes with high connectivity.Combined with the small RNA library,the above four candidate genes were screened for targeting miRNAs,namely FGFR3(Bta-mir-296-5p),NFKB1(Bta-mir-15b),MAP3K5(Bta-mir-375)and TAB1(Bta-mir-15b,Bta-mir-11988 and Bta-mir-7180).Real-time PCR verification showed that the expression levels of Bta-mir-15b,Bta-mir-375,TAB1 and MAP3K5 were higher in the testis,and the expression of other genes and miRNAs was lower in the testis.In summary,20 candidate genes and five candidate miRNAs related to spermatogenesis were screened,among which TAB1 and Bta-mir-15b,MAP3K5 and Bta-mir-375s may be important candidate genes and miRNAs affecting spermatogenesis.

Key words: Blcattle black cattle, Testicles, Transcriptome, mRNA, miRNA

CLC Number:

MA Jiaxu, LIU Ruili, MIAO Xiuping, WANG Xiuyuan, MEI Yanfang, ZHAO Qiuyue, ZOU Lei, XIAO Chaozhu, ZHANG Renzheng, BAI Xuejin, DONG Yajuan. Whole Transcriptome Sequencing was Used to Explore Genes and miRNAs Related to Spermatogenesis in Blcattle Black Cattle[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(3): 225-238. doi: 10.7668/hbnxb.20195752.

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References 55

[1]	王美桀, 刘兴旺, 白曼. lncRNA在哺乳动物雄性生殖中作用的研究进展[J]. 中国畜牧兽医, 2024, 51(7):2963-2972.doi:10.16431/j.cnki.1671-7236.2024.07.022.
	Wang M J, Liu X W, Bai M. Research progress on the role of lncRNA in mammalian male reproduction[J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7):2963-2972.
[2]	孙武, 马世科, 孙永刚, 韩银仓. 睾丸发育过程概述及生殖生物学研究意义[J]. 青海畜牧兽医杂志, 2022, 52(3):40-43.doi:10.3969/j.issn.1003-7950.2022.03.013.
	Sun W, Ma S K, Sun Y G, Han Y C. Overview of testicular development process and significance of reproductive biology research[J]. Chinese Qinghai Journal of Animal and Veterinary Sciences, 2022, 52(3):40-43.
[3]	朱春红, 陶志云, 王志成, 宋卫涛, 刘宏祥, 章双杰, 徐文娟, 顾昊天, 王逸飞, 李慧芳. 健康成年公鸭睾丸大小比较及其相关调控基因表达分析[J]. 农业生物技术学报, 2024, 32(11):2603-2613.doi:10.3969/j.issn.1674-7968.2024.11.014.
	Zhu C H, Tao Z Y, Wang Z C, Song W T, Liu H X, Zhang S J, Xu W J, Gu H T, Wang Y F, Li H F. Comparison of the size of testes in healthy adult male duck(Anas platyrhynchos)and analysis of the expression of related regulatory genes[J]. Journal of Agricultural Biotechnology, 2024, 32(11):2603-2613.
[4]	孙武, 罗静, 李发弟, 李万宏, 乐祥鹏. 基于转录组学筛选与哺乳动物睾丸发育相关基因及调控网络的研究进展[J]. 畜牧兽医学报, 2018, 49(9):1810-1817.doi:10.11843/j.issn.0366-6964.2018.09.002.
	Sun W, Luo J, Li F D, Li W H, Yue X P. Progress of screening genes and regulating network related to testis development in mammals based on transcriptomics[J]. Acta Veterinaria et Zootechnica Sinica, 2018, 49(9):1810-1817. doi: 10.11843/j.issn.0366-6964.2018.09.002
[5]	梅艳芳, 刘瑞莉, 韩明轩, 张文琦, 陈振鹏, 王秀源, 苗秀萍, 肖超柱, 张任政, 董雅娟, 柏学进. 基于转录组测序技术挖掘影响布莱凯特黑牛睾丸大小的功能基因[J]. 华北农学报, 2024, 39(1):191-200.doi:10.7668/hbnxb.20194430.
	Mei Y F, Liu R L, Han M X, Zhang W Q, Chen Z P, Wang X Y, Miao X P, Xiao C Z, Zhang R Z, Dong Y J, Bai X J. Analysis of reproduction function gene of testicle in different size black cattle based on transcriptome sequencing[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1):191-200. doi: 10.7668/hbnxb.20194430
[6]	马剑峰, 陈思宇, 师愉倩, 沈林園, 朱砺, 甘麦邻. 转录组测序解析瘦肉型和脂肪型公猪睾丸的基因表达模式差异[J]. 中国畜牧杂志, 2024, 60(8):191-198.doi:10.19556/j.0258-7033.20240430-05.
	Ma J F, Chen S Y, Shi Y Q, Shen L Y, Zhu L, Gan M L. Transcriptome sequencing analysis of gene expression patterns in lean and fat boar testes[J]. Chinese Journal of Animal Science, 2024, 60(8):191-198.
[7]	Gong W, Pan L L, Lin Q, Zhou Y Y, Xin C Q, Yu X M, Cui P, Hu S N, Yu J. Transcriptome profiling of the developing postnatal mouse testis using next-generation sequencing[J]. Science China Life Sciences, 2013, 56(1):1-12.doi:10.1007/s11427-012-4411-y. URL
[8]	周慧萍. CCDC189在精子发生中的作用及分子机制研究[D]. 扬州: 扬州大学, 2024.doi:10.27441/d.cnki.gyzdu.2024.000123.
	Zhou H P. The role and molecular mechanism of CCDC189 in spermatogenesis[D]. Yangzhou: Yangzhou University, 2024.
[9]	Wei X C, Wang X G, Yang C H, Gao Y P, Zhang Y R, Xiao Y, Ju Z H, Jiang Q, Wang J P, Liu W H, Li Y Q, Gao Y D, Huang J M. CFAP58 is involved in the sperm head shaping and flagellogenesis of cattle and mice[J]. Development, 2024, 151(7):dev202608.doi:10.1242/dev.202608.
[10]	郭睿, 薛社普, 韩代书. 精子发生相关基因的研究进展[J]. 中国组织化学与细胞化学杂志, 2004, 13(1):114-119.doi:10.3870/j.issn.1004-1850.2004.01.024.
	Guo R, Xue S P, Han D S. Research progress on genes related to spermatogenesis[J]. Chinese Journal of Histochemistry and Cytochemistry, 2004, 13(1):114-119.
[11]	Kotaja N. microRNAs and spermatogenesis[J]. Fertility and Sterility, 2014, 101(6):1552-1562.doi:10.1016/j.fertnstert.2014.04.025. pmid: 24882619
[12]	Hayashi K, Kaneda M, Tang F C, Hajkova P, Lao K Q, O'Carroll D, Das P P, Tarakhovsky A, Miska E A, Surani M A. Chuva de Sousa Lopes S M, microRNA biogenesis is required for mouse primordial germ cell development and spermatogenesis[J]. PLoS One, 2008, 3(3):e1738.doi:10.1371/journal.pone.0001738. URL
[13]	Hong X M, Luense L J, McGinnis L K, Nothnick W B, Christenson L K. Dicer1 is essential for female fertility and normal development of the female reproductive system[J]. Endocrinology, 2008, 149(12):6207-6212.doi:10.1210/en.2008-0294. pmid: 18703631
[14]	Suh N, Baehner L, Moltzahn F, Melton C, Shenoy A, Chen J, Blelloch R. microRNA function is globally suppressed in mouse oocytes and early embryos[J]. Current Biology, 2010, 20(3):271-277.doi:10.1016/j.cub.2009.12.044. pmid: 20116247
[15]	Liu W M, Niu Z R, Li Q, Pang R T K, Chiu P C N, Yeung W S. microRNA and embryo implantation[J]. American Journal of Reproductive Immunology, 2016, 75(3):263-271.doi:10.1111/aji.12470. URL
[16]	Vidigal J A, Ventura A. Embryonic stem cell miRNAs and their roles in development and disease[J]. Seminars in Cancer Biology, 2012, 22(5/6):428-436.doi:10.1016/j.semcancer.2012.04.009. URL
[17]	Ding H S, Liu M, Zhou C F, You X B, Su T, Yang Y B, Xu D Q. Integrated analysis of miRNA and mRNA expression profiles in testes of Duroc and Meishan boars[J]. BMC Genomics, 2020, 21(1):686.doi:10.1186/s12864-020-07096-7. pmid: 33008286
[18]	廖珂. 牦牛、普通牛和犏牛睾丸组织microRNA的比较研究[D]. 成都: 西南民族大学, 2016.
	Liao K. Comparative analysis of microRNA in yak,ordinary cattle,and cattle-yak testis tissue[D]. Chengdu: Southwest Minzu University, 2016.
[19]	董雅娟. 牛[M]. 北京: 中国农业出版社, 2016.
	Dong Y J. Cattle[M]. Beijing: China Agriculture Press, 2016.
[20]	杜炫星, 牛敏敏, 赵清, 蔡波, 卢运运, 魏久锋, 张虎芳. 李白盾蚧转录组分析及SSR位点开发[J]. 环境昆虫学报, 2023, 45(5):1318-1332.doi:10.3969/j.issn.1674-0858.2023.05.18.
	Du X X, Niu M M, Zhao Q, Cai B, Lu Y Y, Wei J F, Zhang H F. Analysis of the transcriptome and development of SSR loci in Pseudaulacaspisprunicola(Maskell)[J]. Journal of Environmental Entomology, 2023, 45(5):1318-1332.
[21]	毕楷杰, 孙耀国, 杨敏生, 张军. 银腺杨不同部位叶片形态、生理及转录差异分析[J]. 植物遗传资源学报, 2023, 24(3):829-842.doi:10.13430/j.cnki.jpgr.20220924002.
	Bi K J, Sun Y G, Yang M S, Zhang J. Leaf morphology and physiological analysis of different parts of silver glandular poplar[J]. Journal of Plant Genetic Resources, 2023, 24(3):829-842.
[22]	纪会, 王会, 柴志欣, 王吉坤, 王嘉博, 罗晓林, 姬秋梅, 信金伟, 钟金城. 牦牛不同年龄肌肉组织microRNA表达谱及生物信息学分析[J]. 畜牧兽医学报, 2019, 50(5):957-971.doi:10.11843/j.issn.0366-6964.2019.05.007.
	Ji H, Wang H, Chai Z X, Wang J K, Wang J B, Luo X L, Ji Q M, Xin J W, Zhong J C. Differential expression profile and bioinformatics analysis of miRNAs in yak muscle tissue during development[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(5):957-971. doi: 10.11843/j.issn.0366-6964.2019.05.007
[23]	杨远潇, 字向东. 玻璃化冷冻对牦牛未成熟卵母细胞发育能力及COC转录组的影响[J]. 畜牧兽医学报, 2020, 51(2):288-298.doi:10.11843/j.issn.0366-6964.2020.02.010.
	Yang Y X, Zi X D. Effect of vitrification on developmental competence of immature oocytes and COC transcriptome of yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2):288-298. doi: 10.11843/j.issn.0366-6964.2020.02.010
[24]	张宇, 王金开, 陈小林, 李其利, 唐利华, 黄穂萍, 郭堂勋, 玉延华. 水稻响应白叶枯病菌侵染的转录组分析[J]. 南方农业学报, 2023, 54(3):815-828.doi:10.3969/j.issn.2095-1191.2023.03.017.
	Zhang Y, Wang J K, Chen X L, Li Q L, Tang L H, Huang S P, Guo T X, Yu Y H. Transcriptome analysis of rice in response to the infection by Xanthomonas oryzae pv.oryzae[J].Journal of Southern Agriculture, 2023, 54(3):815-828.
[25]	赵锐, 董茗秀, 陈安静, 高燕丽. 毛竹热休克蛋白70(HSP70) 基因家族的鉴定与表达模式分析[J]. 农业生物技术学报, 2023, 31(10):2058-2071.doi:10.3969/j.issn.1674-7968.2023.10.006.
	Zhao R, Dong M X, Chen A J, Gao Y L. Identification and expression pattern analysis of heat shock protein 70(HSP70)gene family in Phyllostachys edulis[J]. Journal of Agricultural Biotechnology, 2023, 31(10):2058-2071.
[26]	Yao R Y, Lu T T, Liu J M, Li W H, Weng X, Yue X P, Li F D. Variants of ADPGK gene and its effect on the male reproductive organ parameters and sperm count in Hu sheep[J]. Animal Biotechnology, 2023, 34(3):664-671.doi:10.1080/10495398.2021.1995400. URL
[27]	Cao M L, Xiong L, Wang X D, Guo S K, Hu L Y, Kang Y D, Wu X Y, Bao P J, Chu M, Liang C N, Pei J, Guo X. Comprehensive analysis of differentially expressed mRNAs,circRNAs,and miRNAs and their ceRNA network in the testis of cattle yak,yak,and cattle[J]. Genomics, 2024, 116(4):110872.doi:10.1016/j.ygeno.2024.110872. URL
[28]	Ou Y, Dores C, Rodriguez-Sosa J R, van der Hoorn F A, Dobrinski I. Primary cilia in the developing pig testis[J]. Cell and Tissue Research, 2014, 358(2):597-605.doi:10.1007/s00441-014-1973-y. pmid: 25107611
[29]	Dacheux D, Martinez G, Broster Reix C E, Beurois J, Lores P, Tounkara M, Dupuy J W, Robinson D R, Loeuillet C, Lambert E, Wehbe Z, Escoffier J, Amiri-Yekta A, Daneshipour A, Hosseini S H, Zouari R, Ben Mustapha S F, Halouani L, Jiang X H, Shen Y, Liu C Y, Thierry-Mieg N, Septier A, Bidart M, Satre V, Cazin C, Kherraf Z E, Arnoult C, Ray P F, Toure A, Bonhivers M, Coutton C. Novel axonemal protein ZMYND12 interacts with TTC29 and DNAH1,and is required for male fertility and flagellum function[J]. eLife, 2023,12:RP87698.doi:10.7554/eLife.87698.
[30]	Harris T P, Schimenti K J, Munroe R J, Schimenti J C. IQ motif-containing G(Iqcg)is required for mouse spermiogenesis[J]. G3, 2014, 4(2):367-372.doi:10.1534/g3.113.009563. URL
[31]	Dupuis S, Girault M S, Le Beulze M, Ialy-Radio C, Bermúdez-Guzmán L, Ziyyat A, Barbaux S. The lack of Tex44 causes severe subfertility with flagellar abnormalities in male mice[J]. Cellular & Molecular Biology Letters, 2024, 29(1):74.doi:10.1186/s11658-024-00587-5.
[32]	Zhang H J, Li M X, Sun H R, Yang W, Ye M X, Li H, Meng Y G. 4SC-202 exerts an anti-tumor effect in cervical cancer by targeting PRLR signaling pathway[J]. Journal of Molecular Histology, 2022, 53(6):891-902.doi:10.1007/s10735-022-10105-6. pmid: 36272045
[33]	Du J P, Zheng L Y, Chen S S, Wang N, Pu X, Yu D, Yan H X, Chen J X, Wang D L, Shen B, Li J, Pan S B. NFIL3 and its immunoregulatory role in rheumatoid arthritis patients[J]. Frontiers in Immunology, 2022, 13:950144.doi:10.3389/fimmu.2022.950144. URL
[34]	Alvarez-Guaita A, Patel S, Lim K, Haider A, Dong L, Conway O J, Ma M K L, Chiarugi D, Saudek V, O'Rahilly S, Savage D B. Phenotypic characterization of Adig null mice suggests roles for adipogenin in the regulation of fat mass accrual and leptin secretion[J]. Cell Reports, 2021, 34(10):108810.doi:10.1016/j.celrep.2021.108810. URL
[35]	Eto K. Nociceptin and meiosis during spermatogenesis in postnatal testes[J]. Vitamins & Hormones, 2015, 97:167-186.doi:10.1016/bs.vh.2014.10.003.
[36]	Santos-Ahmed J, Brown C, Smith S D, Weston P, Rasoulpour T, Gilbert M E, Hixon M L. Akt1 protects against germ cell apoptosis in the postnatal mouse testis following lactational exposure to 6-N-propylthiouracil[J]. Reproductive Toxicology, 2011, 31(1):17-25.doi:10.1016/j.reprotox.2010.09.012. pmid: 20951798
[37]	Yan Q, Wang Q, Zhang Y, Yuan L G, Hu J J, Zhao X X. The novel-m0230-3p miRNA modulates the CSF1/CSF1R/ras pathway to regulate the cell tight junctions and blood testis barrier in yak[J]. Cells, 2024, 13(15):1304.doi:10.3390/cells13151304. URL
[38]	Ricci G, Catizone A. Pleiotropic activities of HGF/c-met system in testicular physiology:paracrine and endocrine implications[J]. Frontiers in Endocrinology, 2014, 5:38.doi:10.3389/fendo.2014.00038.
[39]	Luo D D, He Z, Yu C X, Guan Q B. Role of p38 MAPK signalling in testis development and male fertility[J]. Oxidative Medicine and Cellular Longevity, 2022, 2022(1): 6891897.doi:10.1155/2022/6891897.
[40]	Sambyal K, Singh R V. Production aspects of testosterone by microbial biotransformation and future prospects[J]. Steroids, 2020, 159:108651.doi:10.1016/j.steroids.2020.108651. URL
[41]	Ewen K A, Olesen I A, Winge S B, Nielsen A R, Nielsen J E, Graem N, Juul A, Expression of FGFR3 during human testis development and in germ cell-derived tumours of young adults[J]. The International Journal of Developmental Biology, 2013, 57(2/3/4):141-151.doi:10.1387/ijdb.130022er. URL
[42]	Ornitz D M, Itoh N. The fibroblast growth factor signaling pathway[J]. Wiley Interdisciplinary Reviews Developmental Biology, 2015, 4(3):215-266.doi:10.1002/wdev.176. URL
[43]	Lustofin S, Kaminska A, Brzoskwinia M, Pardyak L, Pawlicki P, Szpregiel I, Bilinska B, Hejmej A. Follicle-stimulating hormone regulates Notch signalling in the seminiferous epithelium of continuously and seasonally breeding rodents[J]. Reproduction,Fertility,and Development, 2022, 34(7):560-575.doi:10.1071/RD21237.
[44]	Diniz P, Leites I, R Batista M, Torres A C, Mateus L, Lopes-da-Costa L, Silva E. Characterization of expression patterns and dynamic relocation of Notch proteins during acrosome reaction of bull spermatozoa[J]. Scientific Reports, 2024, 14:14925.doi:10.1038/s41598-024-65950-0. pmid: 38942812
[45]	Ishikura Y, Ohta H, Sato T, Murase Y, Yabuta Y, Kojima Y, Yamashiro C, Nakamura T, Yamamoto T, Ogawa T, Saitou M. In vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells[J]. Cell Stem Cell, 2021, 28(12):2167-2179.e9.doi:10.1016/j.stem.2021.08.005. pmid: 34496297
[46]	Zovoilis A, Pantazi A, Smorag L, Opitz L, Riester G S, Wolf M, Zechner U, Holubowska A, Stewart C L, Engel W. Embryonic stem cell-related miRNAs are involved in differentiation of pluripotent cells originating from the germ line[J]. Molecular Human Reproduction, 2010, 16(11):793-803.doi:10.1093/molehr/gaq053. pmid: 20566704
[47]	Chen Y, Li X L, Liao H J, Leung X, He J B, Wang X, Li F P, Yue H X, Xu W M. CFTR mutation compromises spermatogenesis by enhancing miR-15b maturation and suppressing its regulatory target CDC25A[J]. Biology of Reproduction, 2019, 101(1):50-62.doi:10.1093/biolre/ioz062. pmid: 30985893
[48]	Guo S H, Cong B L, Zhu L Y, Zhang Y, Yang Y, Qi X L, Wang X G, Xiao L F, Long C, Xu Y X, Sheng X H. Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters[J]. BMC Genomics, 2024, 25(1):1029.doi:10.1186/s12864-024-10836-8. pmid: 39497056
[49]	Xia W L, Mruk D D, Lee W M, Cheng C Y. Differential interactions between transforming growth factor-beta3/TbetaR1,TAB1,and CD2AP disrupt blood-testis barrier and Sertoli-germ cell adhesion[J]. The Journal of Biological Chemistry, 2006, 281(24):16799-16813.doi:10.1074/jbc.M601618200. URL
[50]	Melo T P, Fortes M R S, Bresolin T, Mota L F M, Albuquerque L G, Carvalheiro R. Multitrait meta-analysis identified genomic regions associated with sexual precocity in tropical beef cattle[J]. Journal of Animal Science, 2018, 96(10):4087-4099.doi:10.1093/jas/sky289. pmid: 30053002
[51]	Wang J, Yao S P, Diao Y P, Geng Y, Bi Y L, Liu G Y. miR-15b enhances the proliferation and migration of lung adenocarcinoma by targeting BCL2[J]. Thoracic Cancer, 2020, 11(6):1396-1405.doi:10.1111/1759-7714.13382. pmid: 32220063
[52]	Du S M, Qu H, Zhang Y, Zhu S H, Wang Y, Zhang S P, Wang Z, Yang Q, Fu S B, Dong K X. miR-375 promotes cisplatin sensitivity of lung adenocarcinoma[J]. Pathology-Research and Practice, 2023, 249:154765.doi:10.1016/j.prp.2023.154765. URL
[53]	Li Z M, Zheng Z Z, Ruan J, Li Z, Zhuang X, Tzeng C M. Integrated analysis miRNA and mRNA profiling in patients with severe oligozoospermia reveals miR-34c-3p downregulates PLCXD3 expression[J]. Oncotarget, 2016, 7(33):52781-52796.doi:10.18632/oncotarget.10947. pmid: 27486773
[54]	Wang Q Y, Feng J N, Wang J, Zhang X Y, Zhang D L, Zhu T, Wang W D, Wang X Q, Jin J F, Cao J X, Li X Y, Peng H, Li Y, Shen B F, Zhang J Y. Disruption of TAB1/p38α interaction using a cell-permeable peptide limits myocardial ischemia/reperfusion injury[J]. Molecular Therapy, 2013, 21(9):1668-1677.doi:10.1038/mt.2013.90. pmid: 23877036
[55]	Young J C, Wakitani S, Loveland K L. TGF-β superfamily signaling in testis formation and early male germline development[J]. Seminars in Cell & Developmental Biology, 2015, 45:94-103.doi:10.1016/j.semcdb.2015.10.029.

组别 Group	体质量/kg Body weight	睾丸质量/g Testicular weight	睾丸长径/cm Testicular long diameter	睾丸短径/cm Testicular short diameter
BT	243.67±14.43a	186.00±32.19a	10.50±0.87a	4.50±0.00a
ST	259.33±12.57b	110.67±6.43b	9.00±0.00b	3.67±0.29b

组别 Group	体质量/kg Body weight	睾丸质量/g Testicular weight	睾丸长径/cm Testicular long diameter	睾丸短径/cm Testicular short diameter
BT	243.67±14.43a	186.00±32.19a	10.50±0.87a	4.50±0.00a
ST	259.33±12.57b	110.67±6.43b	9.00±0.00b	3.67±0.29b

基因 Gene	引物序列(5'-3') Primer sequence (5'-3')	退火温度/℃ Annealing temperature
GAPDH	F:GATGCTGGTGCTGAGTATGT	60
	R:GCAGAAGGTGCAGAGATGAT
U6	F:GGAACGATACAGAGAAGATTAGG	60
	R:TGGAACGCTTCACGAATTTGCG
TAB1	F:CGACCTACTCAGTGCTGCCAA	60
	R:ATCAGCACCAGGAAGCCTGTC
NFKB1	F:ACTGCCGTGCAGGATGAGAAC	60
	R:AAGCCAGATGTGACTTCCAGCA
MAP3K5	F:AGGTCCAGGTGGTGCTCTTTG	60
	R:TGTCTGTACCGCCTTTCGGAT
FGFR3	F:TGTACGTGCTGGTGGAGTACG	60
	R:GGCAGGTGTCGAAGGAGTAGT
ELP5	F:GGTGACCCTGAGCGGTACAAT	58
	R:AGAACCACTGAGTCTGGAGGGT
TXNDC2	F:CCCCAAACCAAATCCTCCCA	60
	R:CTGTGCAGGGCAGTTTTGAGTG
RNASE1	F:TGCTCCCAGAAAAATGTTGCC	60
	R:ACAGTTGGGGTACTTGGAGCTG
Bta-mir-11988	F:GGGACGACAGAGGATGAGAAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-15b	F:TAGCAGCACATCATGGTTTACAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-375	F:CGTTCGGCTCGCGTGAAAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-296-5p	F:GCCCCCCCCAATCCTAAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-105b	F:CAAATGCTCAGACTCCTTGGTA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-124b	F:ACGCGGTGAATGCCAAGAAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-106a	F:AAAAGTGCTTACAGTGCAGGTAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-7180	F:CTCTGGGTGTGTACCCTCAAA	58
	R:GTATCAACGCAGAGTACTTT

基因 Gene	引物序列(5'-3') Primer sequence (5'-3')	退火温度/℃ Annealing temperature
GAPDH	F:GATGCTGGTGCTGAGTATGT	60
	R:GCAGAAGGTGCAGAGATGAT
U6	F:GGAACGATACAGAGAAGATTAGG	60
	R:TGGAACGCTTCACGAATTTGCG
TAB1	F:CGACCTACTCAGTGCTGCCAA	60
	R:ATCAGCACCAGGAAGCCTGTC
NFKB1	F:ACTGCCGTGCAGGATGAGAAC	60
	R:AAGCCAGATGTGACTTCCAGCA
MAP3K5	F:AGGTCCAGGTGGTGCTCTTTG	60
	R:TGTCTGTACCGCCTTTCGGAT
FGFR3	F:TGTACGTGCTGGTGGAGTACG	60
	R:GGCAGGTGTCGAAGGAGTAGT
ELP5	F:GGTGACCCTGAGCGGTACAAT	58
	R:AGAACCACTGAGTCTGGAGGGT
TXNDC2	F:CCCCAAACCAAATCCTCCCA	60
	R:CTGTGCAGGGCAGTTTTGAGTG
RNASE1	F:TGCTCCCAGAAAAATGTTGCC	60
	R:ACAGTTGGGGTACTTGGAGCTG
Bta-mir-11988	F:GGGACGACAGAGGATGAGAAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-15b	F:TAGCAGCACATCATGGTTTACAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-375	F:CGTTCGGCTCGCGTGAAAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-296-5p	F:GCCCCCCCCAATCCTAAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-105b	F:CAAATGCTCAGACTCCTTGGTA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-124b	F:ACGCGGTGAATGCCAAGAAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-106a	F:AAAAGTGCTTACAGTGCAGGTAA	58
	R:GTATCAACGCAGAGTACTTT
Bta-mir-7180	F:CTCTGGGTGTGTACCCTCAAA	58
	R:GTATCAACGCAGAGTACTTT

样品 Sample	原始数据 Raw data	高质量数据(比例/%) High-quality data (Percentage)	Q20/%	Q30/%	GC含量/% GC content
G01977	11 763 576 000	11 679 509 986(99.28)	97.80	93.78	54.59
G01977	11 763 576 000	11 679 509 986(99.28)	97.80	93.78	54.59
G06733	10 802 985 900	10 715 638 472(99.19)	97.69	93.48	54.77
G01987	12 970 445 400	12 858 417 663(99.13)	98.03	94.20	54.10
G01970	14 511 260 700	14 387 304 603(99.14)	97.74	93.79	56.55
G06970	12 875 203 500	12 754 631 752(99.06)	97.65	93.63	57.75
G06970	15 949 562 700	15 806 166 906(99.10)	97.89	94.10	56.01

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