Seed-specific Expression of BnGPAT9 Gene Controlled by Napin Promoter in Arabidopsis thaliana

doi:10.7668/hbnxb.20190708

Abstract

Abstract: Glycerol-3-phosphate acyltransferase(GPAT) genes encode enzymes involved in glycerolipid biosynthesis in plants. To investigate the increase of oil content in rapeseed,the yield of oilseed rape and improve the fatty acid composition of rapeseed oil,we cloned Napin promoter and triacylglycerol synthesisrelated gene BnGPAT9,constructed pBI121-Napin-BnGPAT9 seed-specific expression vector and transformed into Arabidopsis thaliana,analyzed fatty acid composition and oil content in seeds. Using soil Agrobacterium-mediated transformation of Arabidopsis thaliana,gas chromatography and soxhlet extraction method had been analyzed the fatty acid composition and oil content in Arabidopsis seeds. PlantCARE analysis of the Napin promoter showed that the cloned promoter could achieve seed-specific expression,as it had lots of characteristic elements,such as TATA-box,CAAT-box,ABRE and GCN4 motifs. The results showed that oleic acid (C18:1) content was increased by 0.70 to 1.01 percent point,linoleic acid (C18:2) and erucic acid(C22:1) content were decreased,oil content was increased by 1.91 to 2.56 percent point in Arabidopsis seeds,suggesting that overexpress BnGPAT9 gene may effectively increase seed oil content and fatty acid composition in rape. This study lays a foundation for the application of BnGPAT9 gene in the improvement of rapeseed oil.

Key words: Brassica napus L., Napin promoter, BnGPAT9 gene, Plant expression vector, Oil content

CLC Number:

S634.03
Q78

XING Man, HONG Bo, ZHANG Zerong, ZHANG Xiyu, CHEN Tuo, WU Ningrou, GUAN Mei, WU Xianmeng, GUAN Chunyun, XIONG Xinghua. Seed-specific Expression of BnGPAT9 Gene Controlled by Napin Promoter in Arabidopsis thaliana[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 1-7. doi: 10.7668/hbnxb.20190708.

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