QTL Mapping of Protein and Oil Contents in Soybean Using Recombinant Inbred Population

doi:10.7668/hbnxb.20190517

Abstract

Abstract: Soybean is the main source of protein and oil in edible plants, improvement of protein and oil content are the major objective of soybean breeding programs. Mapping QTL by molecular markers has important theoretical significance and practical value for soybean molecular breeding. Here, two associated soybean recombinant inbred line (RIL) populations, Dongnong L13×Henong 60 (RIL6013) and Dongnong L13×Heihe 36 (RIL3613), comprising 134,156 RILs, respectively, were grown in three environments and their protein and oil contents were measured. The genetic map was constructed with 150,137 SSR markers respectively, and the complete interval mapping (ICIM) was used to map the oil and protein content. Through the analysis of phenotypic data, the phenotypic variation coefficients of protein and oil content in the two RIL populations were large, and there were significant differences in protein content and oil content in different environments. 8 QTLs for protein content were found in both RIL3613 and RIL6013 on 7 linkages, and 5 for oil content were found on 5 linkages. 1 QTL for oil content was repeatedly detected in 2 planting environments. In the located QTLs,7 protein content and 3 oil content QTLs were consistent with previous studies, and the other three(qPro-G-1, qOil-C1-1, qOil-H-1)were newly discovered in this study and were specific to the genetic background of this study. These results are of great significance for molecular design breeding of soybean quality traits.

Key words: Soybean, Protein content, Oil content, RIL, QTL

CLC Number:

S565.03

TIAN Yu, WANG Yanshu, ZHANG Jianan, XU Shichao, DONG Quanzhong, LI Wenbin, LI Wenxia, NING Hailong. QTL Mapping of Protein and Oil Contents in Soybean Using Recombinant Inbred Population[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 106-112. doi: 10.7668/hbnxb.20190517.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20190517

http://www.hbnxb.net/EN/Y2020/V35/I4/106

References

[1] Palomeque L, Liu L J, Li W B, Hedges B, Cober E R, Rajcan I. QTL in mega-environments:Ⅱ. agronomic trait QTL co-localized with seed yield QTL detected in a population derived from a cross of high-yielding adapted×high-yielding exotic soybean lines[J]. Theoretical and Applied Genetics,2009, 119(3):429-436.doi:10.1007/s00122-009-1048-8.
[2] Wang X Z, Jiang G L, Green M,Scott R A, Hyten D L,Cregan P B. Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean[J]. Molecular Breeding,2012,30(2):1163-1179.doi:10.1007/s11032-012-9704-0.
[3] Rossi M E, Orf J H, Liu L J, Dong Z M, Rajcan I. Genetic basis of soybean adaptation to North American vs. Asian mega-environments in two independent populations from Canadian×Chinese crosses[J]. Theoretical and Applied Genetics, 2013,126(7):1809-1823.doi:10.1007/s00122-013-2094-9.
[4] Qi Z M, Wu Q, Han X, Sun Y N, Du X Y, Liu C Y, Jiang H W, Hu G H, Chen Q S. Soybean oil content QTL mapping and integrating with meta-analysis method for mining genes[J]. Euphytica, 2011, 179:499-514.doi:10.1007/s10681-011-0386-1.
[5] Diers B W, Keim P, Fehr W R, Shoemaker R C. RFLP analysis of soybean seed protein and oil content[J]. Theoretical and Applied Genetics, 1992, 83(5):608-612. doi:10.1007/BF00226905.
[6] Lee S H, Bailey M A R, Mian M A R, Carter T E, Shipe E R, Ashley D A, Parrott W A, Hussey R S, Boerma H R. RFLP loci associated with soybean seed protein and oil content across populations and locations[J]. Theoretical and Applied Genetics, 1996, 93(5-6):649-657.doi:10.1007/bf00224058.
[7] 葛振宇, 刘晓冰, 刘宝辉, 阿部纯, 马凤鸣, 孔凡江. 大豆种子蛋白质和油份性状的QTL定位[J].大豆科学, 2011, 30(6):901-905. Ge Z Y, Liu X B, Liu B H, Abe J, Ma F M, Kong F J. QTL mapping of protein and oil content in soybean[J]. Soybean Science, 2011, 30(6):901-905.
[8] Liang H Z, Yu Y L, Wang S F, Lian Y, Wang T F, Wei Y L, Gong P T, Liu X Y, Fang X J, Zhang M C. QTL mapping of isoflavone,oil and protein contents in soybean (Glycine max L.Merr.)[J]. Agricultural Sciences in China, 2010, 9(8):1108-1116.doi:10.1016/S1671-2927(09)60197-8.
[9] Chung J, Babka H L, Graef G L, Staswick P E, Lee D J, Cregan P B, Shoemarker R C, Specht J E. The seed protein oil and yield QTL on soybean linkage group I[J]. Crop Science, 2003, 43(3):1053-1067.doi:10.2135/cropsci2003.1053.
[10] Wang X Z, Jiang G L, Green M, Scott R A, Song Q J, Hyten D L, Cregan P B. Identification and validation of quantitative trait loci for seed yield, oil and protein contents in two recombinant inbred line populations of soybean[J]. Mol Genet Genomic s,2014, 289(5):935-949. doi:10.1007/s00438-014-0865-x.
[11] Han Y P, Teng W L, Wang Y, Zhao X, Wu L, Li D M, Li W B. Unconditional and conditional QTL underlying the genetic interrelationships between soybean seed isoflavone, and protein or oil contents[J]. Plant Breeding, 2015, 134(3):300-309.doi:10.1111/pbr.12259.
[12] Mao T T, Jiang Z F, Han Y P, Teng W L, Zhao X, Li W B. Identification of quantitative trait loci underlying seed protein and oil contents of soybean across multi-genetic backgrounds and environments[J]. Plant Breeding, 2013, 132(6):630-641.doi:10.1111/pbr.12091.
[13] Ning H L, Yuan J Q, Dong Q Z, Li W B, Xue H, Wang Y S, Tian Y, Li W B. Identification of QTLs related to the vertical distribution and seed-set of pod number in soybean (Glycine max (L.) Merr.)[J]. PLoS One,2018, 13(4):e0195830.doi:10.1371/journal.pone.0195830.
[14] Buckler E S, Holland J B, Bradbury P J, Acharya C B,Brown P J, Browne C, Ersoz E, Flint-Garcia S, Garcia A, Glaubitz J C,Garcia A, Glaubitz J C, Goodman M M, Harjes C, Guill K, Kroon D E, Larsson S, Lepak N K, Li H H, Mitchell S E, Pressoir G, Peiffer J A, Rosas M O, Rocheford T R, Romay M C, Romero S, Salvo S, Villeda H S, da Silva H S, Sun Q, Tian F, Upadyayula N, Ware D, Yates H, Yu J M, Zhang Z W, Kresovich S, McMullen M D. The genetic architecture of maize flowering time[J]. Science, 2009, 325(5941):714-718.doi:10.1126/science.1174276.
[15] 李君. 小麦关联重组自交系群体遗传图谱的构建及主要品质性状的QTL定位[D].泰安:山东农业大学, 2011.doi:10.7666/d.d143624. Li J. Construction of genetic linkage maps and QTL mapping for quality related traits using two associated RIL populations of wheat[D].Taian:Shandong Agricultural University,2011.
[16] Kabelka E A, Diers B W, Fehr W R, LeRoy A R, Baianu I C, You T, Neece D J, Nelson R L. Putative alleles for increased yield from soybean plant introductions[J]. Crop Science, 2004, 44(3):784-791.doi:10.2135/cropsci2004.0784.
[17] Jun T H, Van K, Kim M Y, Lee S H, Walker D R.Association analysis using SSR markers to find QTL for seed protein content in soybean[J]. Euphytica,2008,162:179-191.doi:10.1007/s10681-007-9491-6.
[18] Panthee D R, Pantalone V R, West D R, Saxton A M, Sams C E.Quantitative trait loci for seed protein and oil concentration, and seed size in soybean[J]. Crop Science,2005,45(5):2015-2022. doi:10.2135/cropsci2004.0720.
[19] Tajuddin T, Watanabe S, Masuda R, Harada K, Kawano S. Application of near infrared transmittance spectroscopy to the estimation of protein and lipid contents in single seeds of soybean recombinant inbred lines for quantitative trait loci analysis[J]. Journal of Near Infrared Spectroscopy, 2002, 10(1):315.doi:10.1255/jnirs.348.
[20] Gai J Y, Wang Y J, Wu X L, Chen S Y. A comparative study on segregation analysis and QTL mapping of quantitative traits in plants-with a case in soybean[J]. Frontiers of Agriculture in China, 2007, 1(1):1-7.doi:10.1007/s11703-007-0001-3.
[21] Reinprecht Y, Poysa V W, Yu K F, Rajcan I, Ablett G R, Pauls K P. Seed and agronomic QTL in low linolenic acid, lipoxygenase-free soybean (Glycine max (L.) Merrill) germplasm[J]. Genome, 2006, 49(12):1510-1527.doi:10.1139/g06-112.
[22] Brummer E C, Graef G L, Orf J H, Wilcox J R, Shoemaker R C. Mapping QTL for seed protein and oil content in eight soybean populations[J]. Crop Science, 1997, 37(2):370-378.doi:10.2135/cropsci1997.0011183x003700020011x.
[23] Hyten D L, Pantalone V R, Sams C E, Saxton A M, Ellis D L, Stefaniak T R, Schmidt M E. Seed quality QTL in a prominent soybean population[J]. Theoretical and Applied Genetics, 2004, 109(3):552-561.doi:10.1007/s00122-004-1661-5.
[24] Qiu B X, Arelli P R, Sleper D A. RFLP markers associated with soybean cyst nematode resistance and seed composition in a ‘Peking’×‘Essex’ population[J]. Theoretical and Applied Genetics,1999,98:356-364.doi:10.1007/s001220051080.
[25] Eskandari M, Cober E R, Rajcan I. Genetic control of soybean seed oil:I. QTL and genes associated with seed oil concentration in RIL populations derived from crossing moderately high-oil parents[J]. Theoretical and Applied Genetics, 2013, 126(2):483-495.doi:10.1007/s00122-012-1995-3.
[26] Specht J E, Chase K, Macrander M, Graef G L, Chung J, Markwell J P, Germann M, Orf J H, Lark K G. Soybean response to water:a QTL analysis of drought tolerance[J]. Crop Science,2001, 41(2):493-509.doi:10.2135/cropsci2001.412493x.
[27] Warrington C V, Abdel-Haleem H, Hyten D L, Cregan P B, Orf J H, Killam A S, Bajjalieh N, Li Z, Boerma H R. QTL for seed protein and amino acids in the benning×danbaekkong soybean population[J]. Theoretical and Applied Genetics, 2015,128(5):839-850.doi:10.1007/s00122-015-2474-4.
[28] Sebolt A M, Shoemaker R C, Diers B W. Analysis of a quantitative trait locus allele from wild soybean that increases seed protein concentration in soybean[J]. Crop Science,2000,40(5):1438-1444.doi:10.2135/cropsci2000.4051438x.
[29] Lu W G, Wen Z X, Li H C, Yuan D H, Li J Y, Zhang H, Huang Z W, Cui S Y, Du W J. Identification of the quantitative trait loci (QTL) underlying water soluble protein content in soybean[J]. Theoretical and Applied Genetics,2013,126:425-433.doi:10.1007/s00122-012-1990-8.
[30] Veeranki S,Choubey D. Interferon-inducible p200-family protein IFI16, an innate immune sensor for cytosolic and nuclear double-stranded DNA:regulation of subcellular localization[J]. Molecular Immunology,2012,49(4):567-571.doi:10.1016/j.molimm.2011.11.004.
[31] 侯萌, 齐照明, 韩雪, 辛大伟, 蒋洪蔚, 刘春燕, 吴琼, 隋丽丽, 胡国华, 陈庆山. 大豆蛋白质和油分含量 QTL 定位及互作分析[J]. 中国农业科学, 2014, 47(13):2680-2689.doi:10.3864/j.issn.0578-1752.2014.13.020. Hou M, Qi Z M, Han X, Xin D W, Jiang H W, Liu C Y, Wu Q, Sui L L, Hu G H, Chen Q S. QTL Mapping and interaction analysis of seed protein content and oil content in soybean[J]. Scientia Agricultura Sinica, 2014, 47(13):2680-2689.

Please choose a citation manager

Content to export