Abstract: In order to reveal the correlation between the SlWRKY6 gene and heavy metal stress in plants, the SlWRKY6 gene was cloned from tomato by RT-PCR, the gene sequence number was NM_001365762.1. Bioinformatics analysis showed that the gene contained a complete open reading frame of 1 342 bp, encoding 447 amino acid residues.The protein encoded by this gene contained a WRKY domain structure and belonged to a typical Group Ⅱ subfamily member. Phylogenetic analysis showed that the amino acid sequence of tomato SlWRKY6 and tomato SpWRKY31 was closely related. Tissue-specific analysis showed that the SlWRKY6 gene was expressed in different tissues of tomato, with the highest expression in old leaves. The results of Real-time fluorescence quantitative PCR analysis showed that the SlWRKY6 gene was up-regulated under the stress of three heavy metals(CdCl₂, CuCl₂, HgSO₄), and the expression level of tomato roots was higher under Cd and Cu stress. Under CdCl₂ stress, SlWRKY6 gene was induced significantly higher in tomato roots than in leaves;the expression level of SlWRKY6 gene decreased with the increase of CuCl₂ stress concentration;Under HgSO₄ stress, the expression of this gene in roots and leaves was significantly higher than that in the control(0 h), with the prolongation of HgSO₄ stress, the relative expression of SlWRKY6 gene showed a reverse trend in tomato roots and leaves. In summary, this study obtained the coding region sequence of the SlWRKY6 gene, and preliminarily described the expression of the tomato SlWRKY6 gene under three heavy metal stresses, which provided a research basis for the screening of functional genes in response to heavy metal stress in tomatoes.

Key words: Tomato, SlWRKY6 gene cloning, Bioinformatics, Heavy metals stress, Expression analysis

CLC Number: 

• Q78

• S641.03