Dynamic Transcriptome Analysis of Rice Response to Nitrogen Treatment at Tillering Stage

doi:10.7668/hbnxb.20191406

Abstract

Abstract: Nitrogen is a macronutrient element necessary for the growth and development of rice. By exploring the transcriptional response of rice to nitrogen at tillering stage, screening of nitrogen response genes was used to cultivate rice varieties with high nitrogen use efficiency, and at same time provide precise cultivation of rice transcriptomics guidance for rice cultivation.Using Wuyoudao 4 as the material, the leaves of Wuyoudao 4 were harvested 7, 14, 21 d after nitrogen application, and the dynamic transcriptome analysis was performed.Nitrogen treatment deepened the color of rice leaves at tillering stage, and 3 870 differentially expressed genes(DEGs) were detected. GO analysis showed that these genes were mainly involved in metabolic processes, cellular processes, cell components, catalytic activity and binding. KEGG analysis showed that 22 DEGs were significantly correlated with galactose metabolism, amino sugar and nucleotide glucose metabolism, alanine, aspartic acid and glutamic acid metabolism pathways. In addition, 35 interacting proteins were detected through interacting protein prediction. The above metabolic pathways had different responses to nitrogen at different time points in the tillering stage. After nitrogen treatment, the differentially expressed genes galA, HK, galE(LOC_Os05g51670) and malZ in the early response were mainly involved in the galactose metabolism pathway;The differentially expressed genes HEXA_B, GNPNAT1, GlmS, UAP1, RGP, XYL4, AXS, UXS1, UGDH, RHM, galE(LOC_Os09g15420) and GAUT in the mid-term response were mainly involved in the metabolic pathways of amino sugar and nucleotide sugar;differences in the late response. The expressed genes AsnB, ASL, NadB, GOT2, GLT1 and GlnA were mainly involved in the metabolic pathways of alanine, aspartic acid and glutamate.These results indicated that the above DEGs play different roles in the response to varied nitrogen during rice development temporally.

Key words: Rice, Nitrogen, Transcriptome, Tillering stage

CLC Number:

Q78
S511.03

LIU Tianqi, GAO Hongxiu, XIE Wei, ZHANG Xueqing, CHEN Nana, MEI Xuefeng, XING Jiani, XU Zhenhua, ZHANG Zhongchen. Dynamic Transcriptome Analysis of Rice Response to Nitrogen Treatment at Tillering Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(1): 44-53. doi: 10.7668/hbnxb.20191406.

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