Transcriptome Data Reveals the Specific Deposition of Inosine in Muscle Tissue of Bashang Long-tailed Chickens Regulated by PLA2G4A

doi:10.7668/hbnxb.20194925

Abstract

Abstract:

In order to investigate the regulatory level of PLA2G4A gene on inosine monophosphate(IMP)in the leg muscle tissue of Bashang Long-tailed chicken,and further explore the biological function of PLA2G4A gene in Bashang Long-tailed chicken.The leg muscle tissue of 270-day-old Bashang Long-tailed chicken was categorized based on the inosine monophosphate content,with five samples allocated to each group.Transcriptome sequencing analysis was conducted on the muscle tissue,followed by KEGG enrichment analysis,GO function annotation and protein interaction network analysis.RT-qPCR was used to analyze the mRNA expression level gene in the leg muscle tissue of Bashang Long-tailed chicken.KEGG enrichment analysis showed that the signal pathways related to IMP synthesis included glycerophospholipid metabolism,etherlipid metabolism and arachidonic acid metabolism.GO functional annotation showed that the PLA2G4A gene was involved in 11 biological processes,including glycerophosphate metabolism,etherlipid metabolism,arachidonic acid metabolism and linoleic acid metabolism.Protein interaction revealed that the PLA2G4A gene shared important regulatory enzyme functions in arachidonic acid metabolism and glycerophosphate metabolism.The Real-time Fluorescence Quantitative PCR results showed that there was a significant difference in the expression level of the PLA2G4A gene in the leg muscle tissue of Bashang Long-tailed chicken in the sample.In summary,the PLA2G4A gene may be the main gene affecting IMP deposition in chicken.

Key words: Bashang Long-tailed chicken, Transcriptome, IMP, PLA2G4A, mRNA

HE Lingyu, QIAO Xian, WANG Xinyue, LI Xianglong. Transcriptome Data Reveals the Specific Deposition of Inosine in Muscle Tissue of Bashang Long-tailed Chickens Regulated by PLA2G4A[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(S1): 329-336. doi: 10.7668/hbnxb.20194925.

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References 43

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基因名称 Gene name	登录号 Accession No.	引物序列(5'-3') Primer sequence(5'-3')	扩增长度/bp Amplification length
PLA2G4A	NC_052539.1	F:5'-CACTTCCCGTGACGTACCAG-3' R:5'-TGGATCCAGAAAGACCAGCG -3'	146
β-actin	396526	F:5'-TATTGCTGCGCTCGTTGTTG-3' R:5'-GGGGTACTTCAGGGTCAGGA-3'	199

基因名称 Gene name	登录号 Accession No.	引物序列(5'-3') Primer sequence(5'-3')	扩增长度/bp Amplification length
PLA2G4A	NC_052539.1	F:5'-CACTTCCCGTGACGTACCAG-3' R:5'-TGGATCCAGAAAGACCAGCG -3'	146
β-actin	396526	F:5'-TATTGCTGCGCTCGTTGTTG-3' R:5'-GGGGTACTTCAGGGTCAGGA-3'	199

样本 Sample	原始reads Raw reads	过滤后reads Clean reads	过滤后碱基/Gb Clean bases	错误发现率/% False discovery rate	Q20/%	Q30/%	GC含量/% GC content
A1	50 728 000	48 889 916	7.33	0.03	97.39	93.09	51.85
A2	42 785 508	40 886 724	6.13	0.03	97.28	92.85	51.58
A3	47 588 060	45 744 878	6.86	0.03	97.19	92.63	52.19
A4	52 503 284	50 632 380	7.59	0.03	96.72	91.73	50.37
A5	43 447 046	41 692 442	6.25	0.03	97.44	93.39	52.31
B1	46 702 784	44 980 912	6.75	0.03	97.29	92.8	52.23
B2	41 813 830	40 168 072	6.03	0.03	97.28	92.84	52.61
B3	43 513 932	42 487 038	6.37	0.03	96.88	91.9	52.94
B4	45 702 690	44 466 824	6.67	0.03	97.03	92.29	50.88
B5	46 914 640	45 820 392	6.87	0.03	97.37	93.1	52.39

样本 Sample	原始reads Raw reads	过滤后reads Clean reads	过滤后碱基/Gb Clean bases	错误发现率/% False discovery rate	Q20/%	Q30/%	GC含量/% GC content
A1	50 728 000	48 889 916	7.33	0.03	97.39	93.09	51.85
A2	42 785 508	40 886 724	6.13	0.03	97.28	92.85	51.58
A3	47 588 060	45 744 878	6.86	0.03	97.19	92.63	52.19
A4	52 503 284	50 632 380	7.59	0.03	96.72	91.73	50.37
A5	43 447 046	41 692 442	6.25	0.03	97.44	93.39	52.31
B1	46 702 784	44 980 912	6.75	0.03	97.29	92.8	52.23
B2	41 813 830	40 168 072	6.03	0.03	97.28	92.84	52.61
B3	43 513 932	42 487 038	6.37	0.03	96.88	91.9	52.94
B4	45 702 690	44 466 824	6.67	0.03	97.03	92.29	50.88
B5	46 914 640	45 820 392	6.87	0.03	97.37	93.1	52.39

信号通路 Signal pathway	基因 Genes	表达 Expression	P值 P-value
甘油磷酸脂代谢Glycerophospholipid metabolism	PLA2G4A^*	下调	0.018 8
醚脂代谢Etherlipid metabolism	PLPP1	下调	0.015 4
脂肪酸降解Fattyacid degradation	ACSL1	上调	0.043 4
花生四烯酸代谢Arachidonic acid metabolism	PLA2G2A	下调	0.022 3
戊糖磷酸途径Pentose phosphate pathway	FBP1	下调	0.002 2

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