Polymorphism of the GBE1 Gene and Its Association with Meat Quality Traits in Bashang Long-tailed Chickens

doi:10.7668/hbnxb.20195642

Abstract

Abstract:

To investigate the effect of polymorphisms in the glycogen branching enzyme 1(GBE1)gene on meat quality and flavor traits of Bashang long-tailed chickens.Target fragments were amplified using polymerase chain reaction(PCR)and sequenced directly to identify single-nucleotide polymorphisms(SNPs)in the GBE1 gene.Correlation analysis was performed between the SNPs and meat quality traits.In this study,analysis of 60 individuals revealed four SNP loci in the GBE1 gene(g.96619229T>C,g.96619243T>G,g.96619350C>T,and g.96619544C>T),each exhibiting three genotypes with dominant genotypes identified as TT,TT,CC,and CT,respectively.Chi-square tests confirmed that all loci were in Hardy-Weinberg equilibrium,with polymorphism information content(PIC)values of 0.262,0.262,0.332,and 0.373,indicating moderate polymorphism(0.25<PIC<0.50).Association analysis demonstrated significant correlations between GBE1 genotypes and meat quality traits.At g.96619229T>C,the TC genotype had higher leg muscle myristic acid content,and CC genotype showed significantly higher mean values of L^* in leg muscle compared to other genotypes.At g.96619243T>G,the TG genotype was associated with higher leg muscle myristic acid content,and the GG genotype displayed significantly higher mean values of L^* than others.At g.96619350C>T,the CT genotype had increased myristic acid content and abdominal fat rate but reduced mean values of L^* in leg muscle At g.96619544C>T,the CT genotype showed higher levels of various fatty acids in the leg muscle and superior slaughter performance traits,including carcass weight,eviscerated weight,abdominal fat weight,and leg muscle weight,while the TT genotype exhibited higher postmortem 24-hour pH in leg muscle compared to other genotypes.Linkage disequilibrium analysis identified strong correlations among the first three SNP loci,resulting in six major haplotypes,with TTC being the predominant haplotype(0.708).These findings indicate that SNPs in the GBE1 gene are significantly associated with meat quality traits of Bashang long-tailed chickens.The strong linkage disequilibrium among intronic SNPs and the identification of TTC as a favorable haplotype provide valuable insights for breeding programs aiming to enhance meat quality in this chicken population.

Key words: Bashang long-tailed chicken, GBE1 gene, Meat quality traits, SNP

CLC Number:

WANG Yalan, LIANG Yaxin, ZHANG Xingchen, YU Baojun, LI Xianglong. Polymorphism of the GBE1 Gene and Its Association with Meat Quality Traits in Bashang Long-tailed Chickens[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(6): 212-222. doi: 10.7668/hbnxb.20195642.

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References 39

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基因位点 Gene locus	引物序列(5'—3') Primer sequence(5'—3')	扩增区域 Amplified area	参考序列编号 Reference ID	产物长度/ bp Product length	最佳退火温度/℃ Optimum annealing temperature
g.96619229T>C	F: TGGGCAAGAATCAGCTCAGG	内含子11	rs732755061	636	56.4
	R: CATCTCCTCCAAGCGTGTGA
g.96619243T>G	F: TGGGCAAGAATCAGCTCAGG	内含子11	rs734840457	636	56.4
	R: CATCTCCTCCAAGCGTGTGA
g.96619350C>T	F: TGGGCAAGAATCAGCTCAGG	内含子11	rs315341172	636	56.4
	R: CATCTCCTCCAAGCGTGTGA
g.96619544C>T	F:GGCACTTGTTGGTGATAAGACA	内含子12	rs313212342	251	56.4
	R:CCTGCCAAGCCTTCTGTAGATA

基因位点 Gene locus	引物序列(5'—3') Primer sequence(5'—3')	扩增区域 Amplified area	参考序列编号 Reference ID	产物长度/ bp Product length	最佳退火温度/℃ Optimum annealing temperature
g.96619229T>C	F: TGGGCAAGAATCAGCTCAGG	内含子11	rs732755061	636	56.4
	R: CATCTCCTCCAAGCGTGTGA
g.96619243T>G	F: TGGGCAAGAATCAGCTCAGG	内含子11	rs734840457	636	56.4
	R: CATCTCCTCCAAGCGTGTGA
g.96619350C>T	F: TGGGCAAGAATCAGCTCAGG	内含子11	rs315341172	636	56.4
	R: CATCTCCTCCAAGCGTGTGA
g.96619544C>T	F:GGCACTTGTTGGTGATAAGACA	内含子12	rs313212342	251	56.4
	R:CCTGCCAAGCCTTCTGTAGATA

SNP	等位基因频率 Allele frequency	基因型频率 Genotype frequency	数量 Number
g.96619229T>C	T (0.808)	TT (0.650)	39
	C (0.192)	TC (0.317)	19
		CC (0.033)	2
g.96619243T>G	T (0.808)	TT (0.650)	39
	G (0.192)	TG (0.317)	19
		GG (0.033)	2
g.96619350C>T	C (0.700)	CC (0.517)	31
	T (0.300)	CT (0.367)	22
		TT (0.117)	7
g.96619544C>T	C (0.458)	CC (0.250)	15
	T (0.542)	CT (0.417)	25
		TT (0.333)	20

SNP	等位基因频率 Allele frequency	基因型频率 Genotype frequency	数量 Number
g.96619229T>C	T (0.808)	TT (0.650)	39
	C (0.192)	TC (0.317)	19
		CC (0.033)	2
g.96619243T>G	T (0.808)	TT (0.650)	39
	G (0.192)	TG (0.317)	19
		GG (0.033)	2
g.96619350C>T	C (0.700)	CC (0.517)	31
	T (0.300)	CT (0.367)	22
		TT (0.117)	7
g.96619544C>T	C (0.458)	CC (0.250)	15
	T (0.542)	CT (0.417)	25
		TT (0.333)	20

SNP	多态信息含量 PIC	纯合度 Ho	杂合度 He	有效等位基因数 Ne	χ²	P
g.96619229T>C	0.262	0.690	0.310	3.227	0.029	0.986
g.96619243T>G	0.262	0.690	0.310	3.227	0.029	0.986
g.96619350C>T	0.332	0.580	0.420	2.380	0.967	0.616
g.96619544C>T	0.373	0.503	0.497	2.014	1.552	0.460

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