Abstract:

The transcriptional regulation of spleen tissue at different time points after Salmonella infection in Muscovy ducks was analyzed by transcriptome sequencing technology,to investigate the impact of Salmonella infection on Muscovy ducks.Fourteen-day-old Muscovy ducks were infected with Salmonella enteritidis.After 1,3,and 10 days of infection,spleen tissue samples were collected from the experimental and control groups,and RNA was extracted.The Illumina sequencing platform was used for transcriptome sequencing,and changes in transcriptome,signaling pathways,and functions at different time points were identified through bioinformatics analysis.The results showed that 95,53,and 12 differential genes(P<0.05,| log₂(fold change)|>1)were found in Muscovy ducks infected with Salmonella for 1,3 and 10 days,respectively.GO analysis showed that these differential genes were enriched in biological processes,molecular functions,and cellular components at the three time points.KEGG analysis showed that at 1 day of infection,the most differentially expressed genes of CXCR4,CCL3,GDF9 and CNTFR were enriched in the cytokine-cytokine interaction signaling pathway.During the third day of infection,the differentially expressed genes of ADRA1A,ADRA1b,GRIA1,and MC5R were enriched in the neural active ligand receptor interaction signaling pathway;after 10 days of infection,the differentially expressed gene H2-EB1 participates in multiple signaling pathways,indicating that these signaling pathways and differentially expressed genes may play an important role in the infection process of Salmonella in Muscovy ducks.

Key words: Muscovy duck, Spleen, Transcriptome, Salmonella