Transcriptome Analysis of Biocontrol Bacteria Bacillus tequilensis Treated Camellia oleifera in Different Ways

doi:10.7668/hbnxb.20194720

Abstract

Abstract:

To explore the transcriptome changes after biocontrol bacteria treat Camellia oleifera in different ways,and understand the molecular regulatory mechanism of biocontrol bacteria enhancing C.oleifera resistance.The 2-year-old C.oleifera seedlings were selected as experimental materials,and the biocontrol bacterium Bacillus tequilensis was inoculated onto seedlings by leaf spraying and root irrigation,as well as an equal amount of sterile water was used as a control,and the C.oleifera leaves were taken after 15 days of treatment for transcriptome sequencing analysis.The results indicated that a total of 167 118 913 reads were obtained by transcriptome sequencing,with 19 164 differentially expressed genes(DEGs).Among which there were 4 722 DEGs in sample CK-vs-DZY 6715 and 14 442 DEGs in sample CKG-vs-DZY 6715G,and the number of DEGs common to the two groups of samples was 2 573,with the highest number of unique DEGs to sample CKG-vs-DZY 6715G(11 869),followed by sample CK-vs-DZY 6715(2 149).GO enrichment analysis showed that the DEGs of the two groups of samples were annotated into biological processes,molecular functions,and cellular components,mainly involving metabolic process,cellular process,single biological process,cell,cell part,membrane,binding,catalytic activity,transport activity,etc.KEGG pathway analysis showed that the DEGs of the two groups of samples were enriched into metabolic pathway and biosynthesis of secondary metabolites,while DEGs were also mainly enriched to plant hormone signaling transduction in sample CK-vs-DZY 6715,and the DEGs of sample CKG-vs-DZY 6715G were mainly enriched in the plant pathogen interaction.The main DEGs related to disease resistance were up-regulated expression,including pckA,PGAM,ACOX1,FATB,asnB,ansA,FLS,LAR,AUX1,GID1,ETR,STPK,CDPK,pfkA.However,there were differences in gene expression levels between spray and root irrigation methods,the expression levels of genes related to the biosynthesis of secondary metabolites and plant hormone signal transduction were higher in spray,while those related to metabolic pathway and plant pathogen interactions were higher in root irrigation.Thus,this paper concluded that the biocontrol bacteria Bacillus tequilensis could enhance disease resistance in C.oleifera after treatment with leaf spray or root irrigation,only that the main mechanism of action of the two treatment methods was slightly different.

Key words: Camellia oleifera, Disease resistance, Transcriptome analysis, Biocontrol bacteria

ZHOU Aiting, PENG Ruiqi, GE Bairuixue, WANG Fang, WU Jianrong, MA Huancheng. Transcriptome Analysis of Biocontrol Bacteria Bacillus tequilensis Treated Camellia oleifera in Different Ways[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 194-205. doi: 10.7668/hbnxb.20194720.

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References 45

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样品 Sample	过滤序列 (占原始序列比例) Clean Data		碱基质量 >Q30/% Q30	GC含量/% GC content	唯一比对率/% Unique mapped	总比对率/% Total mapped
CK-1	42 098 092 (99.75%)		92.70	45.23	72.68	77.01
CK-2	38 376 394 (99.75%)		93.18	45.17	73.05	77.38
CK-3	45 139 164 (99.76%)		93.15	45.15	73.19	77.66
CKG-1	40 107 338 (99.78%)		92.87	44.29	72.02	76.82
CKG-2	40 196 048 (99.77%)		92.87	44.09	71.87	76.64
CKG-3	42 229 838 (99.74%)		92.93	44.31	71.98	76.63
DZY6715-1	37 061 360 (99.75%)		92.99	44.33	72.50	76.63
DZY6715-2	40 194 444 (99.78%)		93.00	44.07	73.33	77.19
DZY6715-3	41 171 958 (99.77%)		93.03	44.27	73.42	77.33
DZY6715G-1	37 859 402 (99.76%)		92.91	44.03	73.08	77.19
DZY6715G-2	46 267 092 (99.78%)		92.99	44.05	72.97	76.89
DZY6715G-3	50 655 606 (99.78%)		93.00	44.03	73.15	77.22
比对的BUSCO库里的基因数量 Total BUSCO groups searched		1 440		比对上BUSCO基因的数量 Complete BUSCO	1 017

样品 Sample	过滤序列 (占原始序列比例) Clean Data		碱基质量 >Q30/% Q30	GC含量/% GC content	唯一比对率/% Unique mapped	总比对率/% Total mapped
CK-1	42 098 092 (99.75%)		92.70	45.23	72.68	77.01
CK-2	38 376 394 (99.75%)		93.18	45.17	73.05	77.38
CK-3	45 139 164 (99.76%)		93.15	45.15	73.19	77.66
CKG-1	40 107 338 (99.78%)		92.87	44.29	72.02	76.82
CKG-2	40 196 048 (99.77%)		92.87	44.09	71.87	76.64
CKG-3	42 229 838 (99.74%)		92.93	44.31	71.98	76.63
DZY6715-1	37 061 360 (99.75%)		92.99	44.33	72.50	76.63
DZY6715-2	40 194 444 (99.78%)		93.00	44.07	73.33	77.19
DZY6715-3	41 171 958 (99.77%)		93.03	44.27	73.42	77.33
DZY6715G-1	37 859 402 (99.76%)		92.91	44.03	73.08	77.19
DZY6715G-2	46 267 092 (99.78%)		92.99	44.05	72.97	76.89
DZY6715G-3	50 655 606 (99.78%)		93.00	44.03	73.15	77.22
比对的BUSCO库里的基因数量 Total BUSCO groups searched		1 440		比对上BUSCO基因的数量 Complete BUSCO	1 017

通路 Pathway		基因ID Gene ID		基因注释 Gene annotation	CK-vs-DZY 6715 Log₂(倍数变化) Log₂(fold change)	CKG-vs-DZY 6715G Log₂(倍数变化) Log₂(fold change)
碳水化合物代谢		Unigene0013848		磷酸烯醇丙酮酸激酶基因	2.63	2.06
Carbohydrate		Unigene0053457		乙酰辅酶A合成酶基因	4.81	4.60
metabolism		Unigene0102973		柠檬酸合成酶基因	1.71	1.06
		Unigene0110562		乌头酸水合酶基因	1.63	1.75
		Unigene0000245		乌头酸水合酶基因	1.82	1.53
		Unigene0001474		葡萄糖酸激酶基因	2.86	4.02
		Unigene0005429		6-磷酸果糖激酶基因	1.21	—
		Unigene0012789		6-磷酸葡萄糖酸内酯酶基因	—	1.90
		Unigene0023139		2,3-二磷酸甘油酸依赖性磷酸	—	1.02
				甘油酸突变酶基因
		Unigene0088103		转醛醇酶基因	—	1.28
		Unigene0044302		乙醛脱氢酶基因	2.08	1.32
		Unigene0104211		乙醛脱氢酶基因	—	1.30
		Unigene0063891		乙醛脱氢酶基因	1.08	2.53
		Unigene0005855		乙醛脱氢酶基因	7.44	5.94
脂质代谢		Unigene0011248		酰基辅酶A硫酯酶1/2/4基因	1.74	1.37
Lipid metabolism		Unigene0108211		烯酰基辅酶A水合酶基因	1.14	1.18
		Unigene0036455		乙醇脱氢酶1/7基因	1.57	—
通路 Pathway	基因ID Gene ID		基因注释 Gene annotation		CK-vs-DZY 6715 Log₂(倍数变化) Log₂(fold change)	CKG-vs-DZY 6715G Log₂(倍数变化) Log₂(fold change)
	Unigene0060564		乙醇脱氢酶1/7基因		3.46	1.26
	Unigene0026686		酰基辅酶A氧化酶基因		1.52	1.17
	Unigene0054480		酰基辅酶A氧化酶基因		2.50	1.54
	Unigene0005734		脂酰-ACP硫酯酶B基因		—	1.21
	Unigene0038102		脂酰-ACP硫酯酶B基因		—	3.86
	Unigene0022116		脂酰-ACP硫酯酶B基因		—	9.74
	Unigene0021920		脂酰-ACP硫酯酶B基因		—	10.16
	Unigene0042607		脂酰-ACP硫酯酶B基因		—	10.52
	Unigene0115965		甘油激酶基因		—	1.16
氨基酸代谢	Unigene0070870		天冬酰胺合成酶基因		3.22	7.23
Amino acid	Unigene0053781		天冬酰胺酶基因		5.07	3.44
metabolism	Unigene0109671		天冬氨酸转氨酶基因		3.05	3.07
	Unigene0013390		天冬氨酸转氨酶基因		4.07	2.86
	Unigene0021606		谷氨酸合成酶基因		1.46	1.29
	Unigene0028532		谷氨酸合成酶基因		1.59	1.04
	Unigene0048530		谷氨酸合成酶基因		1.63	—
次生代谢物的生物合成	Unigene0010127		天冬氨酸转氨酶基因		2.55	—
Biosynthesis of	Unigene0109671		天冬氨酸转氨酶基因		3.05	3.07
secondary metabolites	Unigene0013390		天冬氨酸转氨酶基因		4.07	2.86
	Unigene0003354		酪氨酸转氨酶基因		4.61	4.58
	Unigene0085809		酪氨酸转氨酶基因		5.16	5.52
	Unigene0032091		黄酮醇合成酶基因		5.12	—
	Unigene0067475		无色花色素还原酶基因		2.28	—
	Unigene0081414		无色花色素还原酶基因		5.51	—
植物激素信号转导	Unigene0074622		生长素内流载体		2.99	—
Plant hormone	Unigene0016429		乙烯受体		2.67	—
signal transduction	Unigene0016430		乙烯受体		2.06	—
	Unigene0054370		乙烯不敏感蛋白2		1.17	—
	Unigene0044759		乙烯响应转录因子1基因		6.40	—
	Unigene0029358		赤霉素受体		4.38	—
	Unigene0002417		赤霉素受体		1.44	—
	Unigene0043563		赤霉素受体		3.77	—
	Unigene0057053		DELLA蛋白		-1.99	—
	Unigene0018135		DELLA蛋白		-1.85	—
	Unigene0057054		DELLA蛋白		-1.14	—
植物病原互作	Unigene0075498		丝裂原活化蛋白激酶基因		—	1.15
Interaction of	Unigene0058911		钙依赖性蛋白激酶基因		—	1.07
plant pathogens	Unigene0009731		钙依赖性蛋白激酶基因		—	1.38
	Unigene0116242		钙依赖性蛋白激酶基因		—	3.01
	Unigene0047745		钙依赖性蛋白激酶基因		—	4.38
	Unigene0073409		钙依赖性蛋白激酶基因		—	10.71
	Unigene0005187		丝氨酸/苏氨酸蛋白激酶基因		—	1.19
	Unigene0070747		丝氨酸/苏氨酸蛋白激酶基因		—	10.84
	Unigene0115965		甘油激酶基因		—	1.16

通路 Pathway		基因ID Gene ID		基因注释 Gene annotation	CK-vs-DZY 6715 Log₂(倍数变化) Log₂(fold change)	CKG-vs-DZY 6715G Log₂(倍数变化) Log₂(fold change)
碳水化合物代谢		Unigene0013848		磷酸烯醇丙酮酸激酶基因	2.63	2.06
Carbohydrate		Unigene0053457		乙酰辅酶A合成酶基因	4.81	4.60
metabolism		Unigene0102973		柠檬酸合成酶基因	1.71	1.06
		Unigene0110562		乌头酸水合酶基因	1.63	1.75
		Unigene0000245		乌头酸水合酶基因	1.82	1.53
		Unigene0001474		葡萄糖酸激酶基因	2.86	4.02
		Unigene0005429		6-磷酸果糖激酶基因	1.21	—
		Unigene0012789		6-磷酸葡萄糖酸内酯酶基因	—	1.90
		Unigene0023139		2,3-二磷酸甘油酸依赖性磷酸	—	1.02
				甘油酸突变酶基因
		Unigene0088103		转醛醇酶基因	—	1.28
		Unigene0044302		乙醛脱氢酶基因	2.08	1.32
		Unigene0104211		乙醛脱氢酶基因	—	1.30
		Unigene0063891		乙醛脱氢酶基因	1.08	2.53
		Unigene0005855		乙醛脱氢酶基因	7.44	5.94
脂质代谢		Unigene0011248		酰基辅酶A硫酯酶1/2/4基因	1.74	1.37
Lipid metabolism		Unigene0108211		烯酰基辅酶A水合酶基因	1.14	1.18
		Unigene0036455		乙醇脱氢酶1/7基因	1.57	—
通路 Pathway	基因ID Gene ID		基因注释 Gene annotation		CK-vs-DZY 6715 Log₂(倍数变化) Log₂(fold change)	CKG-vs-DZY 6715G Log₂(倍数变化) Log₂(fold change)
	Unigene0060564		乙醇脱氢酶1/7基因		3.46	1.26
	Unigene0026686		酰基辅酶A氧化酶基因		1.52	1.17
	Unigene0054480		酰基辅酶A氧化酶基因		2.50	1.54
	Unigene0005734		脂酰-ACP硫酯酶B基因		—	1.21
	Unigene0038102		脂酰-ACP硫酯酶B基因		—	3.86
	Unigene0022116		脂酰-ACP硫酯酶B基因		—	9.74
	Unigene0021920		脂酰-ACP硫酯酶B基因		—	10.16
	Unigene0042607		脂酰-ACP硫酯酶B基因		—	10.52
	Unigene0115965		甘油激酶基因		—	1.16
氨基酸代谢	Unigene0070870		天冬酰胺合成酶基因		3.22	7.23
Amino acid	Unigene0053781		天冬酰胺酶基因		5.07	3.44
metabolism	Unigene0109671		天冬氨酸转氨酶基因		3.05	3.07
	Unigene0013390		天冬氨酸转氨酶基因		4.07	2.86
	Unigene0021606		谷氨酸合成酶基因		1.46	1.29
	Unigene0028532		谷氨酸合成酶基因		1.59	1.04
	Unigene0048530		谷氨酸合成酶基因		1.63	—
次生代谢物的生物合成	Unigene0010127		天冬氨酸转氨酶基因		2.55	—
Biosynthesis of	Unigene0109671		天冬氨酸转氨酶基因		3.05	3.07
secondary metabolites	Unigene0013390		天冬氨酸转氨酶基因		4.07	2.86
	Unigene0003354		酪氨酸转氨酶基因		4.61	4.58
	Unigene0085809		酪氨酸转氨酶基因		5.16	5.52
	Unigene0032091		黄酮醇合成酶基因		5.12	—
	Unigene0067475		无色花色素还原酶基因		2.28	—
	Unigene0081414		无色花色素还原酶基因		5.51	—
植物激素信号转导	Unigene0074622		生长素内流载体		2.99	—
Plant hormone	Unigene0016429		乙烯受体		2.67	—
signal transduction	Unigene0016430		乙烯受体		2.06	—
	Unigene0054370		乙烯不敏感蛋白2		1.17	—
	Unigene0044759		乙烯响应转录因子1基因		6.40	—
	Unigene0029358		赤霉素受体		4.38	—
	Unigene0002417		赤霉素受体		1.44	—
	Unigene0043563		赤霉素受体		3.77	—
	Unigene0057053		DELLA蛋白		-1.99	—
	Unigene0018135		DELLA蛋白		-1.85	—
	Unigene0057054		DELLA蛋白		-1.14	—
植物病原互作	Unigene0075498		丝裂原活化蛋白激酶基因		—	1.15
Interaction of	Unigene0058911		钙依赖性蛋白激酶基因		—	1.07
plant pathogens	Unigene0009731		钙依赖性蛋白激酶基因		—	1.38
	Unigene0116242		钙依赖性蛋白激酶基因		—	3.01
	Unigene0047745		钙依赖性蛋白激酶基因		—	4.38
	Unigene0073409		钙依赖性蛋白激酶基因		—	10.71
	Unigene0005187		丝氨酸/苏氨酸蛋白激酶基因		—	1.19
	Unigene0070747		丝氨酸/苏氨酸蛋白激酶基因		—	10.84
	Unigene0115965		甘油激酶基因		—	1.16

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