Proteomic Studies on Roots of Upland Cotton Ji 2658 under Verticillium dahliae Stress

doi:10.7668/hbnxb.20190703

Abstract

Abstract: In order to discover the genes related to the infection response of Verticillium dahliae in upland cotton and to reveal the emergency response mechanism of upland cotton to Verticillium dahliae infection,the proteome of cotton roots under Verticillium wilt stress was studied by iTRAQ(Isobaric tags for relative and absolute quantification,iTRAQ),using Ji 2658 as the material,and cotton roots were infected with Verticillium dahliae Vd991 for 1 h as the sample,and water treatment as the control.Forty-six differentially expressed proteins(DEPs)were screened,of which 37 were up-regulated and 9 were down-regulated.Gene ontology annotation analysis showed that the functions of DEPs were divided into 24 categories,with molecular functions accounting for 7 categories,biological processes accounting for 8 categories and cell components accounting for 9 categories.Pathway analysis revealed that DEPs were involved in 32 metabolic pathways.The metabolic P value of phenylalanine metabolism(0.025 308 8)was the smallest.By analyzing the functions of DEPs,it was found that energy metabolism-related proteins,tissue metabolism-related proteins,protein synthesis-related proteins,antioxidant enzymes,nucleoside diphosphate kinase(NDPK)were involved in the process of cotton resistance to Verticillium wilt in the early stage of Verticillium wilt stress(1 h).Moreover,the release of cytochrome c(Cyt-c)indicated that cotton root initiated programmed cell death(PCD)to inhibit the transmission of Verticillium wilt.

Key words: Upland cotton, Root, Verticillium wilt, Proteome, Disease resistance mechanism

CLC Number:

S432.4

LI Dan, ZHAO Cunpeng, LIU Suen, WANG Kaihui, WANG Zhaoxiao, LIU Xu, GUO Baosheng, GENG Junyi. Proteomic Studies on Roots of Upland Cotton Ji 2658 under Verticillium dahliae Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 322-331. doi: 10.7668/hbnxb.20190703.

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