Molecular Identification of Co-infection of Pepper mild mottle virus and Cucumber mosaic virus on Pepper in Kashgar, Xinjiang

doi:10.7668/hbnxb.20191552

Abstract

Abstract: During 2018 to 2019,pepper plants showed typical symptoms of dwarf,yellowing,curling,chlorosis and rolling up of leaves were found in Kashgar,Xinjiang Uygur Autonomous region. To identify the virus that causing pepper virus disease,total RNA was isolated from the diseased tissues with typical virus symptoms,and reverse transcription(RT)-PCR assays were performed with specific primers for Cucumber mosaic virus (CMV)and Pepper mild mottle virus (PMMoV). The expected 735 bp CMV and 472 bp PMMoV fragments were amplified from diseased pepper tissue samples,respectively. Sequence analysis showed 95%,99% similarity with that of CMV(South Korea,GU327368.1)and PMMoV(Japan,AB276030.1),respectively. The results showed that pepper plants were co-infected by CMV and PMMoV in Kashgar,Xinjiang region.

Key words: Pepper, Cucumber mosaic virus, Pepper mild mottle virus, Co-infection, RT-PCR

CLC Number:

S432.4

CHAI Ali, CHEN Lida, XU Shuai, PATIGULI Aisimutuola, WANG Lili, SHI Yanxia, XIE Xuewen, LI Lei, LI Baoju. Molecular Identification of Co-infection of Pepper mild mottle virus and Cucumber mosaic virus on Pepper in Kashgar, Xinjiang[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 332-337. doi: 10.7668/hbnxb.20191552.

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