Bioinformatics Analysis and Expression Profiling of the Aluminum Transporter HmALMT11 in Hydrangea macrophylla

doi:10.7668/hbnxb.20194790

Abstract

Abstract:

Aluminum-activated malate transporter(ALMT)gene family,as a widely distributed gene family in plants,the encoded protein plays an important role in regulating plant root acid secretion and response to aluminum ions.In order to clarify the sequence characteristics and expression features of H. macrophylla Endless Summer(HmALMT11)under abiotic stress, further exploring the biological functions of H. macrophylla Endless Summer, and providing a theoretical basis for the subsequent identification of the function of HmALMT11.HmALMT11 gene was cloned from the leaves of Hydrangea macrophylla Endless Summer,and the bioinformatics,subcellular localization and aluminum stress response analysis of this gene were also carried out.HmALMT11 contained an open reading frame of 1 590 bp and encoded 529 amino acids,with a molecular weight of 59.1 ku and a theoretical isoelectric point of 9.10 was an unstable basic protein containing 6 transmembrane domains.The subcellular localization analysis revealed that the protein encoded by HmALMT11 was specifically targeted to the plasma membrane.The qRT-PCR analysis revealed that treatments with both low(100 μmol/L) and high(800 μmol/L) concentrations of Al₂(SO₄)₃ increased the expression level of HmALMT11 in the roots,stems,and leaves of Hydrangea macrophylla Endless Summer.Notably,the highest expression level was observed in the roots,demonstrating a clear tissue-specific expression pattern.These results suggested that HmALMT11 protein might play an important role during the regulation of aluminum tolerance in hydrangea.

Key words: Hydrangea macrophylla, ALMT, Aluminum stress, Bioinformatics analysis, Subcellular localization, qRT-PCR

WANG Xiaoyue, CHEN Shuangshuang, QI Xiangyu, FENG Jing, CHEN Huijie, SUN Ming, DENG Yanming. Bioinformatics Analysis and Expression Profiling of the Aluminum Transporter HmALMT11 in Hydrangea macrophylla[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(4): 94-101. doi: 10.7668/hbnxb.20194790.

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References 29

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引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
HmALMT11-F1	ATGGCCGCAGATGCAGAGAA
HmALMT11-R1	ATCTGTTCCATGGGAAGGCAAAT
HmALMT11-F2	AGCTTGCATGCCTGCAGGTCGACATGGC
	CGCAGATGCAGAGAAG
HmALMT11-R2	CTCGCCCTTGCTCACCATGGATCCATCTG
	TTCCATGGGAAGGCA
HmALMT11-RTF	GATTCAGACTCCTCGCTCAGTACG
HmALMT11-RTR	GCGGCGGTTCTTAATGCTGTTAG
HmUPL7-RTF	GCCACGTACTCACACTTGCT
HmUPL7-RTR	TCTGTGCCTGCAATCCCTTT

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
HmALMT11-F1	ATGGCCGCAGATGCAGAGAA
HmALMT11-R1	ATCTGTTCCATGGGAAGGCAAAT
HmALMT11-F2	AGCTTGCATGCCTGCAGGTCGACATGGC
	CGCAGATGCAGAGAAG
HmALMT11-R2	CTCGCCCTTGCTCACCATGGATCCATCTG
	TTCCATGGGAAGGCA
HmALMT11-RTF	GATTCAGACTCCTCGCTCAGTACG
HmALMT11-RTR	GCGGCGGTTCTTAATGCTGTTAG
HmUPL7-RTF	GCCACGTACTCACACTTGCT
HmUPL7-RTR	TCTGTGCCTGCAATCCCTTT

类型Type	数值Value
氨基酸数/个Number of amino acids	529
分子质量/ku Molecular weight	59.1
理论pI值Theoretical pI	9.10
带负电荷的残基总数(Asp+Glu)/个Total number of negatively charged residues(Asp+Glu)	57
带正电荷的残基总数(Arg+Lys)/个Total number of positively charged residues(Arg+Lys)	69
分子式Formula	C₂₆₆₅H₄₂₆₀N₇₁₄O₇₆₄S₁₈
原子总数/个Total number of atoms	8 421
脂肪族氨基酸指数Aliphatic index	98.83
亲水性平均系数(GRAVY)Grand average of hydropathicity(GRAVY)	-0.014
不稳定性指数(不稳定蛋白)Instability index (protein as unstable)	36.03

类型Type	数值Value
氨基酸数/个Number of amino acids	529
分子质量/ku Molecular weight	59.1
理论pI值Theoretical pI	9.10
带负电荷的残基总数(Asp+Glu)/个Total number of negatively charged residues(Asp+Glu)	57
带正电荷的残基总数(Arg+Lys)/个Total number of positively charged residues(Arg+Lys)	69
分子式Formula	C₂₆₆₅H₄₂₆₀N₇₁₄O₇₆₄S₁₈
原子总数/个Total number of atoms	8 421
脂肪族氨基酸指数Aliphatic index	98.83
亲水性平均系数(GRAVY)Grand average of hydropathicity(GRAVY)	-0.014
不稳定性指数(不稳定蛋白)Instability index (protein as unstable)	36.03

亚细胞Subcellular location	分值Score
亚细胞Subcellular location	LocDB	PotLocDB	Neural Nets	Pentamers	Integral
细胞核Nuclear	0	0.0	0	0.00	0.00
质膜Plasma membrane	10	1.4	1	0.37	8.17
胞外Extracellular	0	0.0	1	1.13	0.15
细胞质Cytoplasmic	0	0.0	0	0.46	0.00
线粒体Mitochondrial	0	0.0	0	1.85	0.00
内质网Endoplasmic reticulum	0	0.8	0	1.11	1.14
过氧化物酶体Peroxisomal	0	0.0	1	0.00	0.03
高尔基体Golgi	0	0.0	0	0.78	0.00
叶绿体Chloroplast	0	0.0	0	0.04	0.00
液泡Vacuolar	0	0.8	0	0.00	0.50

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