Bioinformatics Analysis and Functional Validation of Aluminum Tolerance of HmMYB73 in Hydrangea macrophylla

doi:10.7668/hbnxb.20195913

Abstract

Abstract:

Hydrangea macrophylla is an ornamental plant with high aluminum(Al) tolerance and strong Al accumulation capacity.Its flower color is easily influenced by soil pH and the Al³⁺ content in sepals,yet the mechanisms underlying its Al tolerance remain poorly understood.The MYB transcription factor family plays a crucial role in plant stress responses.To investigate the function of MYB transcription factors in Al tolerance in H.macrophylla,this study used H.macrophylla 'Endless Summer' as experimental material.Gene cloning,bioinformatics,co-expression network,expression pattern analysis and yeast functional validation of HmMYB73 were conducted.The results showed that HmMYB73 encoded 266 amino acids,contained a typical R2R3 domain,and belonged to the R2R3-MYB subfamily.Genes co-expressed with HmMYB73 were involved in biological functions such as catalytic activity,transporter activity,response to stimulus and detoxification.Under Al treatment,HmMYB73 was significantly upregulated in the roots,leaves,and sepals of H.macrophylla,with the highest expression level observed in the roots,which was 16 times that of the control.Furthermore,overexpression of HmMYB73 significantly enhanced yeast tolerance to aluminum stress.In summary,HmMYB73 may be involved in the biological regulation during aluminum stress in H.macrophylla.

Key words: Hydrangea macrophylla, MYB73, Aluminum tolerance, Co-expression network, Yeast functional validation

CLC Number:

CHEN Shuangshuang, LIU Yiming, CHEN Wenjuan, FENG Jing, CHEN Huijie, LIU Xintong, DU Xiaohua, DENG Yanming. Bioinformatics Analysis and Functional Validation of Aluminum Tolerance of HmMYB73 in Hydrangea macrophylla[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(6): 85-91. doi: 10.7668/hbnxb.20195913.

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Figures/Tables 8

Tab.1 The primers used in this study

引物名称 Name	引物序列(5'—3') Sequence (5'—3')
HmMYB73-RTF	ATACAATCAAATCGGCCGCC
HmMYB73-RTR	CCAATGGCTTCACTGTGCAA
HmUPL7-RTF	GCCACGTACTCACACTTGCT
HmUPL7-RTR	TCTGTGCCTGCAATCCCTTT
HmMYB73-F	ATGGTGTTTCCGGCGAGGAT
HmMYB73-R	CTCAATCCTGCTAATCTCAATACGC
HmMYB73-PF	GGGAATATTAAGCTTGGTACCATGGTGTTTCCGGCGAGGAT
HmMYB73-PR	TAGATGCATGCTCGAGCGGCCGCCTCAATCCTGCTAATCTCAATACGC

Fig.1 Potential phosphorylation site of HmMYB73

Fig.2 The tertiary structure of HmMYB73

Fig.3 Phylogenetic tree of MYB73

Fig.4 Amino acid sequence comparison of MYB73

Fig.5 Co-expression network of hydrangea HmMYB73

Fig.6 Expression level of HmMYB73 in hydrangea under Al treatment Different letters indicate significant differences between different treatment(P<0.05).

Fig.7 Enhancement of Al tolerance by HmMYB73 in yeast A.Phenotypic analysis of yeast;B.Determination of growth curve of yeast under Al₂(SO₄)₃ treatment. Different letters indicate significant differences between different strains in different time(P<0.05).

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