Screening of Innate Immune Candidate Genes in Rongchang Pigs and Landrace

doi:10.7668/hbnxb.20192398

Abstract

Abstract: The aim of this study was to explore the innate immune candidate genes in Rongchang pigs and Landrace. Thymus of newborn Rongchang pigs and Landrace were used as the research objects. The RNA-seq technology was used to analyze the differentially expressed genes in the thymus of Rongchang pigs and Landrace. Then, the GO and KEGG enrichment analysis of differentially expressed genes were performed, and the qRT-PCR was used to verify the accuracy of RNA-seq. The results showed that a total of 421 differentially expressed genes were identified, including 231 up-regulated genes and 190 down-regulated genes of Rongchang pig compared to Landrace. GO enrichment analysis showed that these differentially expressed genes were mainly enriched in the biological processes such as scavenger receptor activity, G protein-coupled receptor binding, T cell activation, regulation of secretion by cell, exocytosis and SNARE complex assembly. KEGG pathway analysis showed that these differentially expressed genes were mainly involved in signal pathways such as cytokine-cytokine receptor interaction, cAMP signal, cysteine and methionine metabolism, phospholipase D signaling and some diseases(African cone disease, cancer).The RT-qPCR results of 6 differentially expressed genes showed that the results of RNA-seq were accurate and reliable. Based on the results of screening, GO and KEGG of differentially expressed genes, TMSB15A and STAB2, SAA1 and HP were candidate genes for thymus innate immunity in Rongchang pigs and Landrace, respectively.

Key words: Pig, Innate immune, RNA-seq, Candidate genes, Disease resistance

CLC Number:

S828.8+9

LONG Xi, ZHAO Jiugang, CHAI Jie, PAN Hongmei, ZHANG Liang, ZHANG Tinghuan. Screening of Innate Immune Candidate Genes in Rongchang Pigs and Landrace[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 373-380. doi: 10.7668/hbnxb.20192398.

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