Screening and Evaluation of Tomato Rootstock Varieties Resistant to Wilt Disease

doi:10.7668/hbnxb.20195809

Abstract

Abstract:

To identify the types of tomato wilt pathogens,select tomato rootstock varieties resistant to wilt disease,and explore the effects of resistant rootstocks on enzyme activity and the number of cultivable microorganisms in the rhizosphere.The results showed that the Fusarium oxysporum f.sp.lycopersici race 3 was the pathogen responsible for tomato wilt in Dingxing area,which has a large tomato cultivation area in Hebei Province.Two high resistance rootstock varieties(TMS150,Jinpeng No.8)and two disease resistant rootstock varieties(Ouzhen 006,CFH-27)were screened using the root injury soaking method.Overall,disease resistant varieties increased the activities of superoxide dismutase,peroxidase,polyphenol oxidase,and catalase,which were positively correlated with the abundance of potential beneficial microorganisms(Bacteria,Actinomycetes,Biocontrol bacteria,Nitrifying bacteria,Ammonifying bacteria,Azotobacter,and Cellulose degrading bacteria)in the rhizosphere soil and negatively correlated with the number of Fungi.This result provided a theoretical basis for the breeding of vegetable rootstock varieties with disease resistance and disease control.

Key words: Tomato wilt disease, Physiological race, Disease resistance, Enzyme activity, Microbial community

CLC Number:

CHEN Lida, XU Ao, WU Xiaolei, GONG Binbin, LI Bohan, LI Jingrui, LYU Guiyun, CHAI Ali, XIE Xuewen, WU Jun, GAO Wei, BEN Haiyan, LI Baoju, GAO Hongbo. Screening and Evaluation of Tomato Rootstock Varieties Resistant to Wilt Disease[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 256-262. doi: 10.7668/hbnxb.20195809.

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Figures/Tables 6

References 35

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引物 Primers	序列(5'-3') Sequence(5'-3')	分子标记 Molecular marker	扩增片段/bp Amplified fragment
uniF	ATCATCTTGTGCCAACTTCAG	Fo	670
uniR	GTTTGTGATCTTTGAGTTGCCA
sp13F	GTCAGTCCATTGGCTCTCTC	Fol 1、3	445
sp13R	TCCTTGACACCATCACAGAG
sp23F	CCTCTTGTCTTTGTCTCACGA	Fol 2、3	518
sp23R	GCAACAGGTCGTGGGGAAAA
sprlF	GATGGTGGAACGGTATGACC	Forl	947
sprlR	CCATCACACAAGAACACAGGA

引物 Primers	序列(5'-3') Sequence(5'-3')	分子标记 Molecular marker	扩增片段/bp Amplified fragment
uniF	ATCATCTTGTGCCAACTTCAG	Fo	670
uniR	GTTTGTGATCTTTGAGTTGCCA
sp13F	GTCAGTCCATTGGCTCTCTC	Fol 1、3	445
sp13R	TCCTTGACACCATCACAGAG
sp23F	CCTCTTGTCTTTGTCTCACGA	Fol 2、3	518
sp23R	GCAACAGGTCGTGGGGAAAA
sprlF	GATGGTGGAACGGTATGACC	Forl	947
sprlR	CCATCACACAAGAACACAGGA

品种 Variety	病情指数 Disease index	抗性评价 Resistance evaluation
TMS150	3.25	HR
Money Maker	68.00	S
欧砧006 Ouzhen 006	18.75	R
美好 Meihao	38.50	T
CFH-11	48.25	S
CFH-25	52.75	S
CFH-26	38.50	T
CFH-27	18.00	R
金鹏8号 Jinpeng No.8	4.75	HR
普罗旺斯 Provence	50.80	S
中杂9号 Zhongza No.9	42.75	S

品种 Variety	病情指数 Disease index	抗性评价 Resistance evaluation
TMS150	3.25	HR
Money Maker	68.00	S
欧砧006 Ouzhen 006	18.75	R
美好 Meihao	38.50	T
CFH-11	48.25	S
CFH-25	52.75	S
CFH-26	38.50	T
CFH-27	18.00	R
金鹏8号 Jinpeng No.8	4.75	HR
普罗旺斯 Provence	50.80	S
中杂9号 Zhongza No.9	42.75	S

酶活性 Enzyme activity	TMS150	Money Maker		欧砧006 Ouzhen 006		美好 Meihao		CFH-11		CFH-25
SOD	12.54±0.12a	10.24±1.03b		11.21±0.43ab		10.10±1.23b		9.60±3.20bc		9.40±3.40bc
POD	138.22±21.00a	106.50±20.32bc		126.40±22.19ab		100.22±18.30bc		102.26±12.50bc		113.40±13.44b
CAT	217.20±35.20a	165.10±25.14b		212.00±40.24a		146.05±22.18bc		129.32±29.90c		126.79±24.62c
PPO	54.23±2.82a	34.30±3.00 bc		44.35±5.02ab		33.25±4.40bc		33.43±5.24bc		30.24±3.82c
酶活性 Enzyme activity	CFH-26		CFH-27		金鹏8号 Jinpeng No.8		普罗旺斯 Provence		中杂9号 Zhongza No.9
SOD	11.75±7.04a		11.12±0.28ab		11.94±0.28a		8.70±3.26c		8.55±6.42c
POD	119.82±13.28b		132.42±27.25a		134.70±26.20a		105.85±19.90bc		98.30±13.44bc
CAT	157.20±36.24b		202.50±48.30a		214.20±44.80a		120.40±38.26c		148.25±22.84bc
PPO	38.80±5.90b		48.50±4.68a		48.90±4.60a		30.45±5.25bc		35.38±7.24bc

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