Differences in Bacterial Communities and Enzyme Activities in Rhizosphere Soil of Different Resistant Oat Varieties under Saline Alkali Stress

doi:10.7668/hbnxb.20195688

Abstract

Abstract:

To explore the relationship between soil bacterial community diversity and soil enzyme activity of Qinqiong(Q) and Magnum(M) under salinity stress treatment,the bacterial community structure and soil enzyme activity of Qinqiong and Magnum for 0,6,12,24,48 h were compared and analyzed by using high-throughput sequencing of bacterial 16S rRNA and colorimetry.The results indicated that at the phylum level,the relative abundances of Actinobacteria and Proteobacteria in the rhizosphere soil of Qinqiong were higher than those in Magnum.At the genus level,the relative abundances of Bacillus and Arthrobacter in the rhizosphere soil of Qinqiong were greater than those in Magnum,while the relative abundance of Pseudomonas was lower compared to Magnum.After 48 hours of saline-alkali stress,the activities of soil polyphenol oxidase(S-PPO),soil catalase(S-CAT),soil dehydrogenase(S-DHA),and soil invertase(S-AI) in the rhizosphere of Qinqiong were 1.32,1.53,1.38,and 1.28 times higher than those of Magnum,respectively.Furthermore,the S-CAT activity exhibited a significant positive correlation with the bacterial ACE and Chao diversity indices.Qinqiong may influence changes in bacterial community structure by altering the relative abundances of dominant bacterial groups,thereby enhancing the bacterial community diversity index and soil enzyme activity.In contrast,the changes in the bacterial community within the rhizosphere of Magnum were not significant.The diversity of soil microbial communities is a critical factor determining the saline-alkali tolerance of oat varieties.

Key words: Oat, Saline-alkali stress, Microbial diversity, Soil enzyme activity

CLC Number:

YIN Yajie, ZHANG Tianran, WANG Rixin, QU Lina, CHEN Naiyu, REN Guoling. Differences in Bacterial Communities and Enzyme Activities in Rhizosphere Soil of Different Resistant Oat Varieties under Saline Alkali Stress[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(1): 145-154. doi: 10.7668/hbnxb.20195688.

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Figures/Tables 9

Fig.1 Growth of oat seedlings under salt-alkali stress Q-6 h,Q-12 h,Q-24 h,Q-48 h represent Qinqiong seedlings after saline-alkali stress for 6,12,24,and 48 h;M-6 h,M-12 h,M-24 h,M-48 h represent Magnum seedlings after saline-alkali stress for 6,12,24,and 48 h.

Fig.2 Rarefaction curves of soil bacterial communities in the oat rhizosphere

Fig.3 OTUs clustering of soil bacterial communities in the oat rhizosphere

Tab.1 Alpha diversity index of bacterial community in Qinqiong rhizosphere soil

指标Index	Q-CK	Q-6 h	Q-12 h	Q-24 h	Q-48 h
Shannon指数Shannon index	1.820 0±0.07a	1.750 0±0.04ab	1.620 0±0.09b	1.710 0±0.08ab	1.720 0±0.08ab
Simpson指数Simpson index	0.240 0±0.02b	0.250 0±0.01b	0.300 0±0.03a	0.270 0±0.02ab	0.260 0±0.02ab
Ace指数 Ace index	35.000 0±1.78a	34.140 0±1.02a	32.590 0±0.45a	33.390 0±1.63a	35.400 0±2.38a
Chao指数 Chao index	35.170 0±2.57a	33.830 0±0.76a	32.500 0±0.87a	32.420 0±1.28a	34.940 0±2.20a
覆盖率 Coverage rate	0.999 9±0.000 07a	0.999 9±0.000 02a	0.999 9±0.000 03a	0.999 9±0.000 03a	0.999 9±0.000 05a

Tab.2 Alpha diversity index of bacterial community in Magnum rhizosphere soil

指标Index	M-CK	M-6 h	M-12 h	M-24 h	M-48 h
Shannon指数Shannon index	6.330±0.08b	6.550±0.02a	6.530±0.05a	6.570±0.07a	6.590±0.04a
Simpson指数Simpson index	0.006±0.000 6a	0.005±0.000 5b	0.004±0.000 2bc	0.004±0.000 2bc	0.004±0.000 2c
Ace指数 Ace index	3 726.500±110.36a	3 881.300±222.05a	3 869.500±367.12a	4 128.800±221.40a	4 084.200±92.65a
Chao指数 Chao index	3 667.700±141.48a	3 812.400±196.28a	3 812.400±196.28a	4 043.300±254.04a	4 034.100±102.38a
覆盖率 Coverage rate	0.979±0.002a	0.975±0.008a	0.974±0.007a	0.978±0.002a	0.979±0.002a

Fig.4 Beta diversity of bacterial community in oats rhizosphere soil A.Principal component analysis of soil microbial community in Qinqiong;B.Principal component analysis of soil microbial community in Magnum;C.Analysis of similarities of soil microbial community in Qinqiong;D.Analysis of similarities of soil microbial community in Magnum.

Fig.5 Relative abundance distribution of rhizosphere soil microbial community A,C.Soil bacterial communities of salt-tolerant oats at phylum,genus level;B,D.Soil bacterial communities of sensitive oats at phylum,genus level.

Fig.6 Enzyme activity differences in oat rhizosphere soil Different lowercase letters indicate significant difference(P<0.05).

Fig.7 Analysis of the diversity of soil microbial communities and soil enzyme activity in the rhizosphere of oats A.Mantel test analysis of Qinqiong rhizosphere bacterial diversity and enzyme activity;B.Mantel test analysis of Magnum rhizosphere bacterial diversity and enzyme activity.

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