Effects of Different Salinity,Alkalinity and pH Value on Sperm Motility and Fertilization Rate of Amur ide

doi:10.7668/hbnxb.20192551

Abstract

Abstract: In order to determine the optimum range of salinity, alkalinity and pH value of sperm motility and fertilization rate of Amur ide. Different NaCl salinity(1‰, 2‰, 3‰, 4‰, 5‰, 6‰, 7‰, 8‰), NaHCO₃ alkalinity(10, 20, 30, 40, 50, 60, 70 mmol/L)and pH value(5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0)were set to stimulate the sperm motility of the Darinor Lake Amur ide.The sperm status of different treatment groups was observed, and the intense movement time, fast movement time and life span of the sperm were measured. Meanwhile, the fertilization rate in different salinity, alkalinity and pH solutions was determined.The results showed as follows. When the salinity ranged from 0 to 4‰, the sperm intense movement, fast movement time and life span increased gradually, and reached the maximum at 4. When the alkalinity was 0-50 mmol/L, the sperm intense movement, fast movement time and life span were prolonged with the increase of alkalinity, and reached the maximum at 50 mmol/L. In the pH value 5.0-7.0, the sperm intense movement, fast movement time and life span increased with the increase of pH, pH value 7.0 reached the maximum. The fertilization rates reached the highest when the salinity was 4‰, the basicity was 30 mmol/L and the pH value was 6.5, respectively. The fertilization rates in different salinity and alkalinity groups were significantly lower than those in the control group. In conclusion, based on the analysis results of different salinity, alkalinity and pH on sperm motility and fertilization rate of Amur ide, it was suggested to select saline-alkali water or fresh water with salinity of 0-4‰, alkalinity of 0-30 mmol/L and pH of 6.0-7.0 in the process of total artificial reproduction and cross breeding of Amur ide. It can obtain higher fertilization rate and seedling survival rate.

Key words: Amur ide, Sperm motility, Rate of fertilization, Salinity, Alkalinity, pH value

CLC Number:

S965

LI Qian, LIANG Liqun, AI Keda, SUN Bo, ZHANG Limin, DONG Zhiguo, CHANG Yumei. Effects of Different Salinity,Alkalinity and pH Value on Sperm Motility and Fertilization Rate of Amur ide[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 381-389. doi: 10.7668/hbnxb.20192551.

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