Biodegradation Characteristics of Chlorpyrifos by Mixed Culture of Bacillus cereus Strains

doi:10.3969/j.issn.1000-7091.2013.02.039

Abstract

Abstract: In the present work,we investigated for the first time the degradation characteristics of chlorpyrifos by the mixed culture of Bacillus cereus strains. The optimal ratio of inoculation volume of the two strains for chlor-pyrifos degradation was established. Further,the effects of the inoculation volume,glucose concentration,initial pH and the initial chlorpyrifos concentration on the degradation efficiency of chlorprifos were determined. Simultaneous-ly,the effect of high salinity on the biodegradation activity of chlorpyrifos was also examined. The results showed that when the ratio(V/V) was 1∶ 1,the degradation rate to 80mg/Lof chlorpyrifos reached the highest. The optimal in-oculum amount of the mixed strains was 8%(V/V) of the total volume. Addition ofglucose stimulated thegrowth of the mixed strains but did not enhance the degradation efficiency of chlorpyrifos. The alkaline environment was prone to form the biomass and to achieve a high degradation rate of chlorpyrifos by the mixed strains. When the initial con-centration of chlorpyrifos was 42mg/L,the residual concentration of chlorpyrifos decreased with the time. However,the degradation curves of chlorpyrifos at high concentrations(108,126mg/L) followed two peaks. The degradation rate of the mixed strains to 80mg/L of chlorpyrifos was above 41% while the sodium chloride concentration ranged from 20g/L to 70g/L. The results suggest that the mixed strains could be used as a potential and efficient chlor-pyrifos degrader for the bioremediation of contaminated sites,especially for the cleanup of high salinity wastewater in the chlorpyrifos production enterprises.

Key words: Chlorpyrifos, Bacillus cereus, Biodegradation, Mixed strains, High salinity

CLC Number:

X172

DUAN Hai-ming,. Biodegradation Characteristics of Chlorpyrifos by Mixed Culture of Bacillus cereus Strains[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 219-224. doi: 10.3969/j.issn.1000-7091.2013.02.039.

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