Electrophoretic Analysis on Lactate Dehydrogenase(LDH)and Malate Dehydrogenase(MDH)Isozymes in Five Different Tissues in Schizothorax macropogon

doi:10.7668/hbnxb.20192209

Abstract

Abstract: This paper was to learn about the biochemical heredity characteristics and to enrich the research data about the germplasm resources of Schizothorax macropogon. Lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)isozymes from five tissues(heart, crystalline lens, muscle, liver and kidney)of Schizothorax macropogon were preliminarily studied by using the vertical polyacrylamide gel electrophoresis. The results showed that:the band numbers of LDH isozymes expressed in heart was 6-7, in crystalline lens 6-10, in muscle 5-7, in liver 8-11 and in kidney, 8-10 respectively, and the band numbers of MDH isozymes expressed in heart was 4-8, in crystalline lens 2-4, in muscle 3-4, in liver 4-5 and in kidney 4-7. LDH-C gene only specificly expressed in liver. The band numbers expressed in liver are the largest in five different tissues. MDH bands expressed in liver presented the darkest and showed the most degree of isozyme activity. LDH isozymes and MDH isozymes showed tissue specificity and individual variations. The tissue specificity mainly reflected in band numbers and degree of isozyme activity. There were correlations between tissue specificity and its specificly physiological functions. In order to adapt the unique habitant, the isozymes of Schizothorax macropogon showed individual variations. The research results can provide theoretical basis for making germplasm standards and the conservation and utilization for Schizothorax macropogon from the biochemical genetics level.

Key words: Schizothorax macropogon, LDH isozymes, MDH isozymes, Tissue specificity, Individual variations

CLC Number:

S917.4

ZHANG Tao, ZHANG Lin, ZHOU Jianguang, GAN Jinhua, HE Li. Electrophoretic Analysis on Lactate Dehydrogenase(LDH)and Malate Dehydrogenase(MDH)Isozymes in Five Different Tissues in Schizothorax macropogon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 390-396. doi: 10.7668/hbnxb.20192209.

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