Electrophoretic Analysis on 3 Kinds of Isozymes in Gymnodiptychus dybowskii

doi:10.7668/hbnxb.20193861

Abstract

Abstract:

This paper was to learn about the biochemical heredity characteristics and to enrich the research data about the germplasm resources of Gymnodiptychus dybowskii.Lactate dehydrogenase(LDH)isozymes,malate dehydrogenase(MDH)isozymes and Esterase(EST)isozymes in five kinds of tissues(heart,crystalline lens,liver,kidney and spleen)of Gymnodiptychus dybowskii were preliminarily studied by using the vertical polyacrylamide gel electrophoresis.The results showed that:LDH isozymes,MDH isozymes and EST isozymes showed tissue specificity and individual variations.The tissue specificity mainly reflected in expression of isozyme sites and degree of isozyme activity,which may be regulated by both heredity and metabolism and were related to the specific physiological functions of various kinds of tissues.LDH-C gene only expressed in liver.Its isozyme bands were near to negative pole in electrophoretogram, which were typical liver bands.In order to adapt the unique habitant,the isozymes of Gymnodiptychus dybowskii showed individual variations.Six supernate type of MDH isozyme bands expressed in heart may be the result of gene redundancy due to tetraploid during the evolution of Gymnodiptychus dybowskii.The research results could provide theoretical basis for the conservation and utilization of germolasm resources for Gymnodiptychus dybowskii from the biochemical genetics level.

Key words: Gymnodiptychus dybowskii, LDH isozymes, MDH isozymes, EST isozymes, Tissue specificity, Individual variations

ZHANG Tao, CHEN Peng, SHA Wenjun, WANG Ranxuan, HE Li, NIU Jiangong, GAN Jinhua. Electrophoretic Analysis on 3 Kinds of Isozymes in Gymnodiptychus dybowskii[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 444-451. doi: 10.7668/hbnxb.20193861.

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References 39

[1]	郭焱. 新疆鱼类志[M]. 乌鲁木齐: 新疆科学技术出版社, 2012.
	Guo Y. Xinjiang fishes[M]. Urumchi: Xinjiang Science and Technology Press, 2012.
[2]	张涛, 牛建功, 刘鸿, 蔡林钢, 焦飞. 喀什河新疆裸重唇鱼标记方法的研究[J]. 水产学杂志, 2022, 35(4):27-31.doi:10.3969/j.issn.1005-3832.2022.04.004.
	Zhang T, Niu J G, Liu H, Cai L G, Jiao F. Tagging techniques for Gymnodiptychus dybowskii releasing in Kashi River[J]. Chinese Journal of Fisheries, 2022, 35(4):27-31.
[3]	蔡林钢, 牛建功, 李红, 刘建. 巩乃斯河新疆裸重唇鱼和斑重唇鱼产卵场微环境研究[J]. 干旱区研究, 2013, 30(1):144-148.doi:10.13866/j.azr.2013.01.013.
	Cai L G, Niu J G, Li H, Liu J. Study on micro-environment at the spawning fields of Gymnodiptychus dybowskii and Diptychus maculates in the kunes river[J]. Arid Zone Research, 2013, 30(1):144-148.
[4]	蔡林钢, 李红, 张人铭, 阿达克, 刘建. 霍尔果斯河水生生物现状初步调查[J]. 水生态学杂志, 2008, 1(6):39-43.doi:10.3969/j.issn.1001-988X.2013.01.022.
	Cai L G, Li H, Zhang R M, A D K, Liu J. The preliminary inverstigation of aquatic organisms in Huoerguosi River[J]. Journal of Hydroecology, 2008, 1(6):39-43.
[5]	牛建功, 刘春池, 刘鸿, 李红, 张涛, 王甜, 蔡林钢. 乌鲁木齐河新疆裸重唇鱼栖息地适宜度初步评价[J]. 浙江海洋学院学报(自然科学版), 2017, 36(5):403-408,413.doi:10.3969/j.issn.1008-830X.2017.05.005.
	Niu J G, Liu C C, Liu H, Li H, Zhang T, Wang T, Cai L G. Assessment of habitat suitability index of Gymnodiptychus dybowskii in Urumqi River[J]. Journal of Zhejiang Ocean University(Natural Science), 2017, 36(5):403-408,413.
[6]	马燕武, 郭焱, 张人铭, 吐尔逊, 谢春刚, 刘建, 李林. 新疆塔里木河水系土著鱼类区系组成与分布[J]. 水产学报, 2009, 33(6):949-956.doi:10.3724/SP.J.00001.
	Ma Y W, Guo Y, Zhang R M, Tu E X, Xie C G, Liu J, Li L. Fauna composition and distribution of aboriginal fish in the Tarim River of Xinjiang Uygur Autonomous Region[J]. Journal of Fisheries of China, 2009, 33(6):949-956.
[7]	牛建功, 蔡林钢, 李红, 马徐发. 伊犁河两种裂腹鱼类基因安全和种群分布的关系[J]. 水产学杂志, 2015, 28(4):1-5.doi:10.3969/j.issn.1005-3832.2015.04.001.
	Niu J G, Cai L G, Li H, Ma X F. Spatial distribution,gonad development and reproductive isolation mechanism in two schizothoracins in Yili River[J]. Chinese Journal of Fisheries, 2015, 28(4):1-5.
[8]	孔磊, 胡文革, 王佳君. 新疆裸重唇鱼染色体的核型及C-带研究初报[J]. 生物学杂志, 2011, 28(1):34-36.doi:10.3969/j.issn.1008-9632.2011.01.034.
	Kong L, Hu W G, Wang J J. Primary study on karyotype and C-banding of Gymnodiptychus dybowskii kessler[J]. Journal of Biology, 2011, 28(1):34-36.
[9]	牛建功, 张涛, 刘鸿, 米乃瓦尔·木衣提, 蔡林钢, 刘春池. 新疆裸重唇鱼胚胎及早期仔鱼发育的形态观察[J]. 水产学杂志, 2019, 32(1):1-6.doi:10.3969/j.issn.1005-3832.2019.01.001.
	Niu J G, Zhang T, Liu H, Mi N W, Cai L G, Liu C C. Morphological observation of embryonic and early larval development of Gymnodiptychus dybowskii[J]. Chinese Journal of Fisheries, 2019, 32(1):1-6.
[10]	张齐, 比拉力·艾山, 李胜忠, 布丽布丽汗, 黄淑萍, 艾力亚木·吐尔衮. 新疆裸重唇鱼胚胎发育观察与孵化技术研究[J]. 新疆农垦科技, 2019, 42(10):31-33.doi:10.3969/j.issn.1001-361X.2019.10.022.
	Zhang Q, Bi L L, Li S Z, Bu L H, Huang S P, Ai L Y. Observation on embryo development and study on hatching technology of naked lipfish in Xinjiang[J]. Xinjiang Farm Research of Science and Technology, 2019, 42(10):31-33.
[11]	刘茂春, 谭刚, 何兴恒, 李璞, 袁聪, 魏祖涛, 陈凡刚. 开都河流域新疆裸重唇鱼胚胎发育观察[J]. 江苏农业科学, 2020, 48(3):175-179.doi:10.15889/j.issn.1002-1302.2020.03.030.
	Liu M C, Tan G, He X H, Li P, Yuan C, Wei Z T, Chen F G. Observation on embryonic development of naked lipped fish in Xinjiang in Kaidu River Basin[J]. Jiangsu Agricultural Sciences, 2020, 48(3):175-179.
[12]	牛建功, 张涛, 刘鸿, 刘春池, 张飞, 蔡林钢. 新疆裸重唇鱼初孵仔鱼寄生虫病及其防治试验[J]. 河北渔业, 2017(11):41-43.doi:10.3969/j.issn.1004-6755.2017.11.011.
	Niu J G, Zhang T, Liu H, Liu C C, Zhang F, Cai L G. Parasitic diseases of newly hatched larvae of naked lipped fish in Xinjiang and their control experiments[J]. Hebei Fisheries, 2017(11):41-43.
[13]	张文润, 郝翠兰, 容梦婕,喀迪尔丁·艾尔肯,穆妮热·喀迪尔,田胜利,刘彦君,高仕文,王璐瑶,岳城. 伊犁河流域新疆裸重唇鱼寄生三代虫的感染情况及空间分布[J]. 淡水渔业, 2021, 51(3):35-40.doi:10.3969/j.issn.1000-6907.2021.03.005.
	Zhang W R, Hao C L, Rong M J, Ka D E, Mu N R, Tian S L, Liu Y J, Gao S W, Wang L Y, Yue C. Infection and spatial distribution of parasitic Gyrodactylus in Gymnodiptychus dybowskii in the Ili River Basin[J]. Freshwater Fisheries, 2021, 51(3):35-40.
[14]	赵雪飞, 米博翰, 梁利群, 马勇强, 孙博, 常玉梅. 新疆裸重唇鱼和斑重唇鱼遗传多样性分析[J]. 水产学杂志, 2019, 32(2):19-24.
	Zhao X F, Mi B H, Liang L Q, Ma Y Q, Sun B, Chang Y M. Genetic diversity analysis of Gymnodiptychus dybowskii and Diptychus maculates[J]. Journal of Fishery Sciences of China, 2019, 32(2):19-24.
[15]	郭飏, 胡文革, 莫超, 武菲, 王翠华, 马得草, 何园. 基于线粒体DNA细胞色素b基因部分序列的新疆裸重唇鱼遗传多样性分析[J]. 大连海洋大学学报, 2016, 31(5):528-532.doi:10.16535/j.cnki.dlhyxb.2016.05.010.
	Guo Y, Hu W G, Mo C, Wu F, Wang C H, Ma D C, He Y. An analysis of genetic diversity in Gymnodiptychus dybowskii based on mt DNA cytochrome b gene sequences[J]. Journal of Dalian Ocean University, 2016, 31(5):528-532.
[16]	王新月, 任道全, 马良, 陈生熬, 刘茂春, 谢从新. 开都河新疆裸重唇鱼生物学特性[J]. 新疆农业科学, 2021, 58(2):375-382.doi:10.6048/j.issn.1001-4330.2021.02.019.
	Wang X Y, Ren D Q, Ma L, Chen S A, Liu M C, Xie C X. Biology on Gymnodiptychus dybowskii in kaidu river[J]. Xinjiang Agricultural Sciences, 2021, 58(2):375-382.
[17]	牛玉娟, 任道全, 陈生熬, 蔡林刚, 牛建功, 谢从新. 伊犁河三支流新疆裸重唇鱼的生长特性研究[J]. 水生态学杂志, 2015, 36(6):59-65.doi:10.15928/j.1674-3075.2015.06.009.
	Niu Y J, Ren D Q, Chen S A, Cai L G, Niu J G, Xie C X. Growth characteristics of gymnodiptychusdybowskii kessler in three tributaries of the Ili River in Xinjiang,China[J]. Journal of Hydroecology, 2015, 36(6):59-65.
[18]	Ardestani G, Rogelio D R, Reyes R, Laude R P. Genetic diversity of two Philippine native freshwater goby species(Perciformes:Gobiidae):implications for conservation[J]. Aquatic Conservation:Marine and Freshwater Ecosystems, 2014, 24(5):592-600.doi:10.1002/aqc.2462. URL
[19]	张涛, 周剑光, 何力. 双须叶须鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的组织特异性分析[J]. 安徽农业大学学报, 2018, 45(2):242-246.doi:10.13610/j.cnki.1672-352x.20180427.028.
	Zhang T, Zhou J G, He L. Preliminary study on lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)isozymes in five different tissues in Ptychobarbus dipogon[J]. Journal of Anhui Agricultural University, 2018, 45(2):242-246.
[20]	贺刚. 湘西盲高原鳅种质特征的研究[D]. 武汉: 华中农业大学, 2008.
	He G. Study on germplasm characteristics of loach in Xiangxi blind plateau[D]. Wuhan: Huazhong Agricultural University, 2008.
[21]	徐钢春, 董晶晶, 聂志娟, 徐跑, 顾若波. 刀鲚不同组织的乳酸脱氢酶同工酶及DNA含量研究[J]. 上海海洋大学学报, 2012, 21(4):481-488.doi:10.3724/SP.J.0000.2009.61078.
	Xu G C, Dong J J, Nie Z J, Xu P, Gu R B. Studies on lactate dehydrogenase isozymes and DNA content in different tissues of Coilia nasus[J]. Journal of Shanghai Ocean University, 2012, 21(4):481-488.
[22]	徐先栋, 廖怀生, 王海华, 朱述淦, 张燕萍, 吴斌, 罗星星. 河川沙塘鳢不同组织6种同工酶分析[J]. 中国农学通报, 2017, 33(25):152-156.doi:10.15889/j.issn.1002-1302.2015.02.074.
	Xu X D, Liao H S, Wang H H, Zhu S G, Zhang Y P, Wu B, Luo X X. Six kinds of isozymes in different tissues of Odontobutis potamophila[J]. Chinese Agricultural Science Bulletin, 2017, 33(25):152-156.
[23]	王红叶, 张娟, 蔡焰值, 卢涛, 秦晓燕. 白甲鱼不同组织同工酶的组织特异性研究[J]. 湖北农业科学, 2011, 50(23):4912-4915.doi:10.3969/j.issn.0439-8114.2011.23.047.
	Wang H Y, Zhang J, Cai Y Z, Lu T, Qin X Y. Study on isozymic tissue specificity of different tissues in Onychostoma Sima[J]. Hubei Agricultural Sciences, 2011, 50(23):4912-4915.
[24]	薛国雄, 官平, 张燕生, 杨靖, 赵淑慧, 吴婷婷, 夏德全. 草鱼LDH同工酶比较酶学和免疫化学性质[J]. 水产学报, 1992, 16(4):357-364.
	Xue G X, Guan P, Zhang Y S, Yang J, Zhao S H, Wu T T, Xia D Q. The comparative enzymology and immunochemical property of ldh isozymes from grass carp ctenopharyngodon idellus[J]. Journal of Fisheries of China, 1992, 16(4):357-364.
[25]	张涛, 张林, 周剑光, 甘金华, 陈建武, 何力. 黄尾鲴形态特征及其同工酶电泳分析[J]. 中国渔业质量与标准, 2019, 9(4):48-55.doi:10.3969/j.issn.2095-1833.2019.04.007.
	Zhang T, Zhang L, Zhou J G, Gan J H, Chen J W, He L. Morphological characteristics and electrophoretic analysis of isozymes in Xenocypris davidi[J]. China Fishery Quality and Standards, 2019, 9(4):48-55.
[26]	蒋俊, 宋超, 周丽青, 胡丽娟, 谢志伟, 刘若晖, 庄平, 张涛. 刀鲚染色体核型及不同组织中的LDH同工酶[J]. 中国水产科学, 2022, 29(2):234-244.doi:10.12264/JFSC2021-0163.
	Jiang J, Song C, Zhou L Q, Hu L J, Xie Z W, Liu R H, Zhuang P, Zhang T. Chromosome karyotype and LDH isoenzyme in different tissues of Coilia nasus[J]. Journal of Fishery Sciences of China, 2022, 29(2):234-244.
[27]	张娟, 王红叶, 蔡焰值, 卢涛, 秦晓燕. 中华倒刺鲃同工酶组织特异性研究[J]. 湖北农业科学, 2011, 50(24):5206-5210.doi:10.3969/j.issn.0439-8114.2011.24.054.
	Zhang J, Wang H Y, Cai Y Z, Lu T, Qin X Y. Study on the isozymic tissue specificity of Spinibarbus sinensis[J]. Hubei Agricultural Sciences, 2011, 50(24):5206-5210.
[28]	尹绍武, 廖经球, 黄海, 雷从改, 陈国华, 张本. 褐点石斑鱼不同组织4种同工酶的研究[J]. 海洋通报, 2007, 26(1):41-44.doi:10.3969/j.issn.1001-6392.2007.01.006.
	Yin S W, Liao J Q, Huang H, Lei C G, Chen G H, Zhang B. Studies on four kinds of isozyme in different tissues of Epinephelus fuscoguttatus[J]. Marine Science Bulletin, 2007, 26(1):41-44.
[29]	蒋晓华, 周铭东, 昝瑞光. 滇池高背鲫鱼早期胚胎发育期间苹果酸脱氢酶(MDH)同工酶的分析[J]. 云南大学学报(自然科学版), 1997, 19(4):383-387.doi:10.1007/BF02951625.
	Jiang X H, Zhou M D, Zan R. Analysis of malate dehydrogenase isozymes during embryogensis of Dianchi high-back crucian carp(Carassius auratus)[J]. Journal of Yunnan University(Natural Sciences Edition), 1997, 19(4):383-387.
[30]	Bailey G S, Wilson A C, Halver J E, Johnson C L. Multiple forms of supernatant malate dehydrogenase in salmonid fishes[J]. The Journal of Biological Chemistry, 1970, 245(22):5927-5940.doi:10.1016/S0021-9258(18)62644-4. URL
[31]	Aspinwall N. Genetic analysis of duplicate malate dehydrogenase loci in the pink salmon,Oncorhynchus gorbuscha[J]. Genetics, 1974, 76(1):64-72. pmid: 4818265
[32]	White G S. Developmental genetics of fishes isozymic analyses of differential gene expression[J]. American Zoologist, 1981, 21:549-572.doi:10.1093/icb/21.2.549. URL
[33]	吴力钊, 王祖熊. 草鱼同工酶发育遗传学研究Ⅱ早期发育过程中的同工酶分析[J]. 遗传学报, 1987, 14(5):387-394.
	Wu L Z, Wang Z X. Study on the developmental genetics of isozymes in grass carp(Ctenopharyngodon idellus)ⅡAnalysis of isozymes during the early development of grass carp[J]. Acta Genetica Sinica, 1987, 14(5):387-394.
[34]	周健博, 李明云. 光唇鱼不同组织的同工酶谱[J]. 水产科学, 2010, 29(4):229-231.doi:10.3969/j.issn.1003-1111.2010.04.010.
	Zhou J B, Li M Y. Isozyme patterns in various tissues of Acrossocheilus fasciatus[J]. Fisheries Science, 2010, 29(4):229-231.
[35]	刘鸿艳. 黑斑原(魚兆)同工酶的研究[D]. 武汉: 华中农业大学, 2006.
	Liu H Y. Study on isozymes of black spot(fish omen)[D]. Wuhan: Huazhong Agricultural University, 2006.
[36]	刘磊, 张晨捷, 彭士明, 高权新, 施兆鸿. 银鲳不同组织中4种同工酶的表达差异[J]. 海洋渔业, 2017, 39(1):68-75.doi:10.15889/j.issn.1002-1302.2015.07.084.
	Liu L, Zhang C J, Peng S M, Gao Q X, Shi Z H. Differential expression of 4 kinds of isozyme in different tissues of Pampus argenteus[J]. Marine Fisheries, 2017, 39(1):68-75.
[37]	张燕萍, 徐先栋, 章海鑫, 李彩刚. 黄尾鲴6种同工酶的组织特异性研究[J]. 中国农学通报, 2016, 32(35):10-14.doi:10.3969/j.issn.1006-3188.2016.02.011.
	Zhang Y P, Xu X D, Zhang H X, Li C G. Six kinds of isozymes in different tissues of Xenocypris davidi[J]. Chinese Agricultural Science Bulletin, 2016, 32(35):10-14.
[38]	张涛, 张林, 周剑光, 甘金华, 何力. 巨须裂腹鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析[J]. 华北农学报, 2021, 36(S1):390-396.doi:10.7668/hbnxb.20192209.
	Zhang T, Zhang L, Zhou J G, Gan J H, He L. Electrophoretic analysis on lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)isozymes in five different tissues in Schizothorax macropogon[J]. Acta Agriculturae Boreali-Sinica, 2021, 36(S1):390-396.
[39]	张涛, 陈建武, 何力. 尖裸鲤5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析[J]. 华北农学报, 2018, 33(S1):89-93.doi:10.7668/hbnxb.2018.S1.015.
	Zhang T, Chen J W, He L. Electrophoretic analysis on lactate dehydrogenase(LDH)and malate dehydrogenase(MDH)in five different tissues of Oxygymnocypris stewartii[J]. Acta Agriculturae Boreali-Sinica, 2018, 33(S1):89-93.

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