Tissue Specificity Express Analysis of Enzyme Genes Related to Lignin Biosynthesis in Suisho Pear Brown Pericarp Mutant

doi:10.7668/hbnxb.2016.05.005

Abstract

Abstract: In order to reveal the expression characteristic of genes (PAL1,PAL2,CAD,4CL,POD) related to lignin biosynthesis in brown pericarp mutant of Suisho pear,and provide the basis for further studying pear brown pericarp formation mechanism.RNA was extracted from different tissues according to RNA extraction kit (EASY spin,Biomed),primers were designed with Primer 5.0 and DNAMAN,expression pattern of genes were analyzed by QRT-PCR.Among the different tissues and organs,the highest relative expression of PAL1,PAL2,CAD,4CL,POD gene appeared in peels.In peels and pulps,the relative expression of POD gene was extremely significantly higher than the other genes.In flowers,leaves and branches phloem,the relative expression of CAD was extremely significantly higher than the other genes.The study suggested that the expression pattern of PAL,CAD,4CL and POD had significant tissue-specific in the five selected tissues and organs,and these genes may be associated with the formation of Suisho pear brown peel closely.

Key words: Brown pericarp mutant of Suisho pear, Key enzymes of lignin biosynthesis, Gene, Reverse transcription-PCR (RT-PCR), Tissue specificity expression

CLC Number:

Q78
S661.03

LIU Bingxu, YU Fengming, ZHANG Libin, WU Junkai, SONG Liqin, XIAO Xiao, DU Xiaodong. Tissue Specificity Express Analysis of Enzyme Genes Related to Lignin Biosynthesis in Suisho Pear Brown Pericarp Mutant[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 28-34. doi: 10.7668/hbnxb.2016.05.005.

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