Effect of StRab5b Gene on Anthocyanin Synthesis in Potato

doi:10.7668/hbnxb.20192727

Abstract

Abstract:

Small GTPases Rabs is a kind of monomer GTP binding protein,which can act as a molecular switch involve in variety of physiological and biochemical reactions in plant cells. In order to explore the relationship between potato small G protein StRab5b and anthocyanin synthesis,the StRab5b gene was overexpressed in potato by Agrobacterium mediated genetic transformation,try to study the effects of StRab5b gene on anthocyanin content and PAL activity. The result showed that overexpression of StRab5b gene significantly increased the anthocyanin content in potato leaves,stems and tubers. Compared with the control,the anthocyanin content in leaves of transgenic potatoes StRab5b-L2,StRab5b-L3 and StRab5b-L5 increased by 42%,24% and 54%,respectively;in stems was increased by 59%,37% and 32%,respectively;in tubers was increased by 118%,82% and 105%,respectively. At the same time,StRab5b gene also promoted the PAL activity,a key enzyme of plant anthocyanin synthesis. Compared with the control,the PAL activity in leaves of transgenic potatoes StRab5b-L2,StRab5b-L3 and StRab5b-L5 increased by 88%,63% and 66%,respectively;in stems increased by 48%,38% and 31%,respectively;in tubers increased by 98%,78% and 64%,respectively. In summary,small GTPases StRab5b increased the content of anthocyanin in potato by regulating PAL activity.

Key words: Potato, Anthocyanin, Small GTPases, StRab5b gene, Genetic transformation, PAL activity

ZHANG Zhiwei, FAN Junchen, KANG Liru, JIA Ruifang, TIAN Zaimin, ZHAO Jun. Effect of StRab5b Gene on Anthocyanin Synthesis in Potato[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 28-33. doi: 10.7668/hbnxb.20192727.

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Figures/Tables 9

Fig.1 The cloning of StRab5b gene M.DNA Marker 2K;1.StRab5b gene.

Fig.2 Identification of T-StRab5b vector digested by KpnⅠand Bgl Ⅱ M.DNA Marker 2000;1.T-StRab5b vector.

Fig.3 Identification of PLG-StRab5b and p1300-221-StRab5b vectors A.PLG-StRab5b/SmaⅠ-SacⅠ:M.DNA Marker 2000 Plus;1.PLG-StRab5b vector.B.p1300-221-StRab5b/SacⅠ-Xba I: M.DNA Marker 15000;1.p1300-221-StRab5b vector.

Fig.4 PCR identification of Agrobacterium tumefaciens containing p1300-221-StRab5b M.DNA Marker 2000;1.Plasmid p1300-221-StRab5b; 2.Negetive control;3-6.Agrobacterium plasmids DNA.

Fig.5 Relative expression of StRab5b gene Different letters represent significant differences(P<0.05). The same as Fig.8-9.

Fig.6 The phenotype of leaves and stem segments after overexpression of StRab5b gene

Fig.7 The phenotype of potato tuber after overexpression of StRab5b gene

Fig.8 The anthocyanin relative content in potato after overexpression of StRab5b gene

Fig.9 The PAL activity in potato after overexpression of StRab5b gene

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