Acta Agriculturae Boreali-Sinica ›› 2022, Vol. 37 ›› Issue (4): 1-10. doi: 10.7668/hbnxb.20192757

Special Issue: Corn Saline-alkali stress Biotechnology Hot Article

• Crop Genetics & Breeding·Germplasm Resources·Biotechnology •     Next Articles

Genome-wide Identification and Expression Response to Stresses Analysis of NRL Gene Family in Zea mays

ZHAO Changjiang1,2,3,4, DU Mengxiang1,2,3,4, SONG Juqi1,2,3,4, XU Shangyuan1,2,3,4, HE Lin1,2,4, XU Jingyu1,2,4, YANG Kejun1,2,3,4, LI Zuotong1,2,3,4,   

  1. 1 College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,China
    2 Key Laboratory of Low-carbon Green Agriculture in Northeastern China,Ministry of Agriculture and Rural Affairs,Daqing 163319,China
    3 Engineering Research Center of Crop Straw Utilization, Heilongjiang Province,Daqing 163319,China
    4 Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement,Daqing 163319,China
  • Received:2022-02-22 Published:2022-08-28
  • Contact: LI Zuotong


NRL(NPH3/RPT2-Like)is a type of light-responsive protein unique to plants and plays a vital role in the phototropic signal pathway. To reveal the NRL gene maize genome's characteristics and expression,we analyzed them using bioinformatics methods combined with qRT-PCR technology. The property,structure,evolution of their encoded proteins,and growth period tissue expression and stress expression were analyzed. 31 ZmNRL genes identified were located in nine maize chromosomes,encoding protein amino acids 464-749 aa,which predicted to have chloroplast,nuclear and cytoplasmic locations. According to protein conservation,ZmNRL family was divided into four categories. Their gene structure also presented certain conservation,the most contained four exons. Analysis of the cis-elements of gene promoters revealed a large number of abscisic acids,jasmonic acid,light response,and anti-oxidation elements,among which G-box and Sp1 were two types of light-related elements. The expression of ZmNRL family genes in tissues during the growth period showed a temporal and spatial specificity,and the majority expression level was not high. Only ZmNRL2,ZmNRL4,ZmNRL24,and ZmNRL29 highly expressed. Furthermore,the characteristic modules were produced based on the data of the tissue co-expression genes. And the GO enrichment analysis of a particular leaf growth module containing six ZmNRL genes,mainly associated with the plastid organization biological processes and rRNA binding molecular functions. The expression of ZmNRL5,ZmNRL7,ZmNRL12,and ZmNRL19 genes were analyzed by qRT-PCR under salt,drought,high temperature,and Rhizoctonia solani inoculation treatments. The results showed that ZmNRL12 was significantly up-regulated in maize seedlings treated with high temperature,while ZmNRL5,ZmNRL7 and ZmNRL19 genes were down-regulated in drought,salt and pathogen treatments. In summary,31 ZmNRL genes were identified in the maize genome. They not only had apparent specific tissue expression but also participated in biotic and abiotic stress responses.

Key words: Maize, NRL, Gene family, Stresses, Expression analysis

Cite this article

ZHAO Changjiang, DU Mengxiang, SONG Juqi, XU Shangyuan, HE Lin, XU Jingyu, YANG Kejun, LI Zuotong. Genome-wide Identification and Expression Response to Stresses Analysis of NRL Gene Family in Zea mays[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 1-10. doi: 10.7668/hbnxb.20192757.

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