Effects of Soil Acidification on Yield,Nitrogen Metabolism, and Related Gene Expression of Maize

doi:10.7668/hbnxb.20192847

Abstract

Abstract:

Yield and nitrogen accumulation of maize will decline under soil acidification,but the physiological mechanism is not clear.Field experiment was conducted with four different soil acidity gradients:nautral acid(pH=7,CK),weak acid(pH=6,T1),medium acid(pH=5,T2)and strong acid(pH=4,T3),comparing yield,nitrogen accumulation,grain protein content,nitrogen metabolism-related enzyme activities,gene expression,nitrate nitrogen,ammonium nitrogen,soluble protein,free amino acid content in leaf and stem of maize.The results showed that compared with CK,the yield of T1,T2 and T3 treatments declined by 4.2%,30.7% and 52.3%,respectively.Grain number per spike decreased by 1.8%,28.1% and 42.8%;grain protein content showed a downward trend with T3 treatment significantly reduced by 14.5%.At the big flare stage,with the increase of soil acidity,nitrogen accumulation in leaves showed a downward trend,it was significantly decreased in T2 and T3 treatment by 28.1% and 56.2%,respectively.In stem,the nitrogen accumulation increased firstly and then decreased.Compared with CK,T1 treatment was significantly increased by 33.1%,and T3 treatment significantly decreased by 65.4%.At the big flare stage, the activities of nitrate reductase, glutamate dehydrogenase and glutamate synthase in leaf and stem under T3 treatment were significantly higher than those in CK, while the activities of glutamine synthase in leaf were significantly decreased. The amino acids in stem decreased first and then increased.With the increase of soil acidity,the expressions of ZmGln2 and ZmFd-GOGAT were up-regulated,which promoted the assimilation of $NH 4 +$ released by photorespiration and $NH 4 +$ produced by $NO 3 -$ reduction;the down-regulated expression of ZmGln1.2-ZmGln1.4, ZmNADH-GOGAT2 in leaf and ZmNADH-GOGAT1 in stem decreased the assimilation of $NH 4 +$ released by catabolism.By up-regulating or down-regulating the expression of relevant genes,maize could promote the production of more free amino acids and soluble proteins during nitrogen metabolism to resist acidification stress,but also reduced the nitrogen accumulation,resulting in lower yield and grain protein content.

Key words: Maize, Soil acidification, Yield, Nitrogen metabolism, Transcriptome

ZHANG Zongxiang, HUANG Zhengrong, WU Xuefan, LIU Nannan, LI Xiaoxiao, DONG Zhaorong, SONG He. Effects of Soil Acidification on Yield,Nitrogen Metabolism, and Related Gene Expression of Maize[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 94-103. doi: 10.7668/hbnxb.20192847.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192847

http://www.hbnxb.net/EN/Y2022/V37/I3/94

Figures/Tables 8

Tab.1 Yield and yield components of maize under different soil acidification treatments

处理 Treatment	产量/(kg/hm²) Yield	穗粒数/(万穗/hm²) Kernel number per ear	千粒质量/g Thousand grain weight
CK	9 370.71±573.06a	490.33±25.17a	315.90±16.73a
T1	8 972.66±524.51a	481.33±19.60a	317.63±8.12a
T2	6 492.57±407.83b	352.67±26.03b	325.60±6.17a
T3	4 473.83±289.49c	280.67±19.14c	328.90±7.48a

Fig.1 Protein content,nitrogen accumulation,nitrogen use efficiency for grain production(NUEg) and nitrogen harvest index of maize at maturity stage under different soil acidification treatments Different lowercase letters indicate significant difference of 5%(P<0.05).The same as Fig.2-4.

Tab.2 Related nitrogen indexes of maize at big flare stage under different soil acidification treatments

器官 Organ	处理 Treatment	铵态氮/(mg/g) N $H 4 +$	硝态氮/(mg/g) N $O 3 -$	可溶性蛋白/(mg/g) Soluble protein	氮素积累量/(kg/hm²) Nitrogen accumulation
叶片	CK	0.062±0.010b	0.017±0.002b	3.50±0.18a	37.18±3.34a
Leaf	T1	0.061±0.002b	0.011±0.001b	3.41±0.49a	34.65±3.20a
	T2	0.078±0.006a	0.080±0.007a	3.18±0.48a	26.75±1.58b
	T3	0.079±0.005a	0.071±0.009a	3.10±0.35a	16.27±1.48c
茎秆	CK	0.021±0.002b	0.307±0.003a	1.03±0.05b	21.07±0.28b
Stem	T1	0.016±0.001b	0.314±0.002a	1.05±0.06b	28.04±1.19a
	T2	0.042±0.005a	0.308±0.005a	1.47±0.10ab	18.83±3.17b
	T3	0.047±0.002a	0.309±0.004a	1.73±0.34a	7.29±0.91c

Tab.2 Related nitrogen indexes of maize at big flare stage under different soil acidification treatments

器官 Organ	处理 Treatment	铵态氮/(mg/g) N $H 4 +$	硝态氮/(mg/g) N $O 3 -$	可溶性蛋白/(mg/g) Soluble protein	氮素积累量/(kg/hm²) Nitrogen accumulation
叶片	CK	0.062±0.010b	0.017±0.002b	3.50±0.18a	37.18±3.34a
Leaf	T1	0.061±0.002b	0.011±0.001b	3.41±0.49a	34.65±3.20a
	T2	0.078±0.006a	0.080±0.007a	3.18±0.48a	26.75±1.58b
	T3	0.079±0.005a	0.071±0.009a	3.10±0.35a	16.27±1.48c
茎秆	CK	0.021±0.002b	0.307±0.003a	1.03±0.05b	21.07±0.28b
Stem	T1	0.016±0.001b	0.314±0.002a	1.05±0.06b	28.04±1.19a
	T2	0.042±0.005a	0.308±0.005a	1.47±0.10ab	18.83±3.17b
	T3	0.047±0.002a	0.309±0.004a	1.73±0.34a	7.29±0.91c

Fig.2 Related enzyme activity in different organs of maize at big flare stage under different soil acidification treatments

Fig.3 Contents of free amino acids in leaves of maize at big flare stage under different soil acidification treatments

Tab.3 Correlation analysis between enzyme activity and nitrogen content of maize at the big flare stag

器官 Organ	指标 Indicators	NR	GS	GDH	GOGAT
叶片	$NH 4 +$	0.349	-0.038	0.612^*	0.371
Leaf	$NO 3 -$	0.440	-0.010	0.570	0.594^*
	可溶性蛋白	0.133	0.172	-0.289	-0.119
	氮素积累量	-0.635^*	0.528	-0.821^**	-0.649^*
茎秆	$NH 4 +$	0.582^*	-0.093	-0.110	-0.113
Stem	$NO 3 -$	0.077	-0.341	0.685^*	0.720^**
	可溶性蛋白	0.237	-0.343	0.592^*	0.732^**
	氮素积累量	0.056	-0.067	-0.652^*	-0.911^**

Tab.3 Correlation analysis between enzyme activity and nitrogen content of maize at the big flare stag

器官 Organ	指标 Indicators	NR	GS	GDH	GOGAT
叶片	$NH 4 +$	0.349	-0.038	0.612^*	0.371
Leaf	$NO 3 -$	0.440	-0.010	0.570	0.594^*
	可溶性蛋白	0.133	0.172	-0.289	-0.119
	氮素积累量	-0.635^*	0.528	-0.821^**	-0.649^*
茎秆	$NH 4 +$	0.582^*	-0.093	-0.110	-0.113
Stem	$NO 3 -$	0.077	-0.341	0.685^*	0.720^**
	可溶性蛋白	0.237	-0.343	0.592^*	0.732^**
	氮素积累量	0.056	-0.067	-0.652^*	-0.911^**

Fig.4 Contents of free amino acids in stems of maize at big flare stage under different soil acidification treatments

Tab.4 Gene relative expression in leaves and stems of maize at big flare stage under different soil acidification treatments

类别 Type	基因名称 Gene name	叶片 Leaf				茎秆 Stem
类别 Type	基因名称 Gene name	CK	T1	T2	T3	CK	T1	T2	T3
硝酸还原酶	NAD(P)H	1	1.56	2.04	2.42	1	0.48	0.72	0.32
Nitrate reductase	NADH1	1	1.00	0.79	0.77	1	1.47	1.18	1.36
	NADH2	1	0.64	0.59	0.27	1	1.18	1.48	1.75
谷氨酸脱氢酶	GDH1	1	1.73	0.89	0.66	1	1.02	1.05	0.61
Glutamate dehydrogenase	GDHA	1	1.70	0.98	1.21	1	1.19	0.82	2.97
	GDH2	1	1.31	1.21	0.39	1	0.85	0.96	0.60
谷氨酰胺合成酶	ZmGln1.1	1	1.44	1.23	2.50	1	0.77	0.14	0.66
Glutamine synthetase	ZmGln1.2	1	0.54	0.26	0.37	1	0.37	0.21	0.11
	ZmGln1.3	1	0.83	0.64	0.37	1	0.58	0.14	0.24
	ZmGln1.4	1	0.74	0.51	0.29	1	0.93	0.29	0.13
	ZmGln1.5	1	1.90	1.47	0.67	1	0.60	0.39	0.19
	ZmGln2	1	1.23	2.34	3.09	1	0.70	1.06	2.87
谷氨酸合成酶	ZmNADH-GOGAT1	1	0.75	1.36	1.30	1	0.57	0.40	0.47
Glutamate synthetase	ZmNADH-GOGAT2	1	0.99	0.52	0.47	1	1.60	0.88	0.95
	ZmFd-GOGAT	1	0.89	1.39	2.69	1	0.55	0.45	0.57

References 39

[1]	陈平平. 酸化土壤对水稻产量与氮利用效率的影响途径研究[D]. 长沙: 湖南农业大学, 2015.
	Chen P P. Effect approach of acidified soil on yield and nitrogen utilization efficiency of rice[D]. Changsha: Hunan Agricultural University, 2015.
[2]	Zhang Y Y, Zhang S Q, Wang R Z, Cai J P, Zhang Y G, Li H, Huang S M, Jiang Y. Impacts of fertilization practices on pH and the pH buffering capacity of calcareous soil[J]. Soil Science and Plant Nutrition, 2016, 62(5/6):432-439.doi: 10.1080/00380768.2016.1226685. doi: 10.1080/00380768.2016.1226685 URL
[3]	Yang Z Y. The shadow price of nitrogen:A dynamic analysis of nitrogen-induced soil acidification in China[J]. China Agricultural Economic Review, 2019, 11(3):489-506.doi: 10.1108/CAER-11-2017-0220. doi: 10.1108/CAER-11-2017-0220 URL
[4]	Guo J H, Liu X J, Zhang Y, Shen J L, Han W X, Zhang W F, Christie P, Goulding K W T, Vitousek P M, Zhang F S. Significant acidification in major Chinese croplands[J]. Science, 2010, 327:1008-1010.doi: 10.1126/science.1182570. doi: 10.1126/science.1182570 pmid: 20150447
[5]	Baquy M A A, Li J Y, Jiang J, Mehmood K, Shi R Y, Xu R K. Critical pH and exchangeable Al of four acidic soils derived from different parent materials for maize crops[J]. Journal of Soils and Sediments, 2018, 18(4):1490-1499.doi: 10.1007/s11368-017-1887-x. doi: 10.1007/s11368-017-1887-x URL
[6]	Nagy J. The effect of soil pH and precipitation variability during the growing season on maize hybrid grain yield in a 17 year long-term experiment[J]. Journal of Hydrology and Hydromechanics, 2011, 59(1):60-67.doi: 10.2478/v10098-011-0005-9. doi: 10.2478/v10098-011-0005-9
[7]	赵静, 韩甜甜, 谢兴斌, 董彦, 吴曼, 梁爱新, 沈向. 酸化梨园土壤酶活性与土壤理化性质之间的关系[J]. 水土保持学报, 2011, 25(4):115-120.doi: 10.13870/j.cnki.stbcxb.2011.04.031. doi: 10.13870/j.cnki.stbcxb.2011.04.031
	Zhao J, Han T T, Xie X B, Dong Y, Wu M, Liang A X, Shen X. Relationship between soil enzymatic activity and soil property in selected acidified pear orchards[J]. Journal of Soil and Water Conservation, 2011, 25(4):115-120.
[8]	Sahay S, Robledo-Arratia L, Glowacka K, Gupta M. Root NRT, NiR,AMT,GS,GOGAT and GDH expression levels reveal NO and ABA mediated drought tolerance in Brassica juncea L[J]. Scientific Reports, 2021, 11(1):7992.doi: 10.1038/s41598-021-86401-0. doi: 10.1038/s41598-021-86401-0 URL
[9]	Pan X Y, Baquy M A A, Guan P, Yan J, Wang R H, Xu R K, Xie L. Effect of soil acidification on the growth and nitrogen use efficiency of maize in Ultisols[J]. Journal of Soils and Sediments, 2020, 20(3):1435-1445.doi: 10.1007/s11368-019-02515-z. doi: 10.1007/s11368-019-02515-z URL
[10]	马庆旭, 王峻, 曹小闯, 孙燕, 孙涛, 吴良欢. 土壤pH对玉米与微生物竞争吸收氨基酸的影响[J]. 应用生态学报, 2017, 28(7):2277-2284.doi: 10.13287/j.1001-9332.201707.033. doi: 10.13287/j.1001-9332.201707.033
	Ma Q X, Wang J, Cao X C, Sun Y, Sun T, Wu L H. Effects of soil pH on the competitive uptake of amino acids by maize and microorganisms[J]. Chinese Journal of Applied Ecology, 2017, 28(7):2277-2284.
[11]	宋航, 周卫霞, 袁刘正, 靳英杰, 李鸿萍, 杨艳, 尤东玲, 李潮海. 光、氮及其互作对玉米氮素吸收利用和物质生产的影响[J]. 作物学报, 2016, 42(12):1844-1852.doi: 10.3724/SP.J.1006.2016.01844. doi: 10.3724/SP.J.1006.2016.01844
	Song H, Zhou W X, Yuan L Z, Jin Y J, Li H P, Yang Y, You D L, Li C H. Effects of light,nitrogen and their interaction on nitrogen absorption,utilization and matter production of maize[J]. Acta Agronomica Sinica, 2016, 42(12):1844-1852. doi: 10.3724/SP.J.1006.2016.01844 URL
[12]	徐晓鹏, 傅向东, 廖红. 植物铵态氮同化及其调控机制的研究进展[J]. 植物学报, 2016, 51(2):152-166.doi: 10.11983/CBB15077. doi: 10.11983/CBB15077
	Xu X P, Fu X D, Liao H. Advances in study of ammonium assimilation and its regulatory mechanism in plants[J]. Chinese Bulletin of Botany, 2016, 51(2):152-166.
[13]	吴永升. 玉米谷氨酰胺合成酶基因Gln1-3、Gln1-4氮利用效率关联性分析[D]. 北京: 中国农业科学院, 2009.
	Wu Y S. Association analysis of glutamine synthetase cenes,Gln1-3 and Gln1-4,with nitrogen use efficiency in maize[D]. Beijing: Chinese Academy of Agricultural Sciences, 2009.
[14]	汪晓丽, 陈平, 封克. 酸性环境下作物幼苗对N O 3 --N和N H 4 +-N的吸收特征[J]. 扬州大学学报(农业与生命科学版), 2007, 28(2):52-56.doi:10.16872/j.cnki.1671-4652.2007.02.014. URL
	Wang X L, Chen P, Feng K. Uptake characteristics of N O 3 --N and N H 4 +-N by different plant seedlings under the low pH condition[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2007, 28(2):52-56.
[15]	童贯和, 梁惠玲. 模拟酸雨及其酸化土壤对小麦幼苗体内可溶性糖和含氮量的影响[J]. 应用生态学报, 2005, 16(8):1487-1492.
	Tong G H, Liang H L. Effects of simulated acid rain and its acidified soil on soluble sugar and nitrogen contents of wheat seedlings[J]. Chinese Journal of Applied Ecology, 2005, 16(8):1487-1492.
[16]	麦博儒, 郑有飞, 梁骏, 刘霞, 李璐, 钟燕川. 模拟酸雨对小麦叶片同化物、生长和产量的影响[J]. 应用生态学报, 2008, 19(10):2227-2233.doi: 10.13287/j.1001-9332.2008.0389. doi: 10.13287/j.1001-9332.2008.0389
	Mai B R, Zheng Y F, Liang J, Liu X, Li L, Zhong Y C. Effects of simulated acid rain on leaf photosynthate,growth,and yield of wheat[J]. Chinese Journal of Applied Ecology, 2008, 19(10):2227-2233.
[17]	仇焕广, 李新海, 余嘉玲. 中国玉米产业:发展趋势与政策建议[J]. 农业经济问题, 2021, 42(7):4-16.doi: 10.13246/j.cnki.iae.2021.07.002. doi: 10.13246/j.cnki.iae.2021.07.002
	Qiu H G, Li X H, Yu J L. China maize industry:Development trends and policy suggestions[J]. Issues in Agricultural Economy, 2021, 42(7):4-16.
[18]	Chen D M, Lan Z C, Bai X, Grace J B, Bai Y F. Evidence that acidification-induced declines in plant diversity and productivity are mediated by changes in below-ground communities and soil properties in a semi-arid steppe[J]. Journal of Ecology, 2013, 101(5):1322-1334.doi: 10.1111/1365-2745.12119. doi: 10.1111/1365-2745.12119 URL
[19]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
	Bao S D. Soil agrochemical analysis[M]. 3rd edition. Beijing: China Agriculture Press, 2000.
[20]	王朝辉, 李生秀. 蔬菜不同器官的硝态氮与水分、全氮、全磷的关系[J]. 植物营养与肥料学报, 1996, 2(2):144-152.doi: 10.11674/zwyf.1996.0208. doi: 10.11674/zwyf.1996.0208
	Wang Z H, Li S X. Relationships between nitrate contents and water,total N as well as total P in different organs of vegetable plants[J]. Journal of Plant Nutrition and Fertilizers, 1996, 2(2):144-152.
[21]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000:192-194.
	Li H S. Principles and techniques of plant physiological and biochemical experiments[M]. Beijing: Higher Education Press, 2000:192-194.
[22]	Zhang C F, Peng S B, Bennett J. Glutamine synthetase and its isoforms in rice spikelets and Rachis during grain development[J]. Journal of Plant Physiology, 2000, 156(2):230-233.doi: 10.1016/S0176-1617(00)80311-9. doi: 10.1016/S0176-1617(00)80311-9 URL
[23]	赵全志, 陈静蕊, 刘辉, 乔江方, 高桐梅, 杨海霞, 王继红. 水稻氮素同化关键酶活性与叶色变化的关系[J]. 中国农业科学, 2008, 41(9):2607-2616.doi: 10.3864/j.issn.0578-1752.2008.09.006. doi: 10.3864/j.issn.0578-1752.2008.09.006
	Zhao Q Z, Chen J R, Liu H, Qiao J F, Gao T M, Yang H X, Wang J H. Relationship between activities of nitrogen assimilation enzymes and leaf color of rice[J]. Scientia Agricultura Sinica, 2008, 41(9):2607-2616.
[24]	Alekseeva T, Alekseev A, Xu R K, Zhao A Z, Kalinin P. Effect of soil acidification induced by a tea plantation on chemical and mineralogical properties of Alfisols in Eastern China[J]. Environmental Geochemistry and Health, 2011, 33(2):137-148.doi: 10.1007/s10653-010-9327-5. doi: 10.1007/s10653-010-9327-5 pmid: 20563880
[25]	Lidon F C, Azinheira H G, Barreiro M G. Aluminum toxicity in maize:Biomass production and nutrient uptake and translocation[J]. Journal of Plant Nutrition, 2000, 23(2):151-160.doi: 10.1080/01904160009382005. doi: 10.1080/01904160009382005 URL
[26]	Tandzi L N, Mutengwa C S, Ngonkeu E L M, Gracen V. Breeding maize for tolerance to acidic soils:A review[J]. Agronomy, 2018, 8(6):84.doi: 10.3390/agronomy8060084. doi: 10.3390/agronomy8060084 URL
[27]	李瑞珂. 土壤pH和氮、磷肥类型对玉米生长发育的影响[D]. 郑州: 河南农业大学, 2018.
	Li R K. Effects of soil pH and different nitrogen and phosphate fertilizer on growth and development of maize[D]. Zhengzhou: Henan Agricultural University, 2018.
[28]	Kidd P S, Proctor J. Effects of aluminium on the growth and mineral composition of Betula pendula Roth[J]. Journal of Experimental Botany, 2000, 51(347):1057-1066.doi: 10.1093/jexbot/51.347.1057. doi: 10.1093/jexbot/51.347.1057 pmid: 10948233
[30]	Dogan I, Ozyigit I I, Demir G. Influence of aluminum on mineral nutrient uptake and accumulation in Urtica pilulifera L.[J]. Journal of Plant Nutrition, 2014, 37(3):469-481.doi: 10.1080/01904167.2013.864306. doi: 10.1080/01904167.2013.864306 URL
[31]	陈平. 介质pH与不同氮素形态对作物幼苗氮素吸收的影响[D]. 扬州: 扬州大学, 2005.
	Chen P. The relationship between nitrogen uptake of crop seedlings and H⁺ concentration of nutrient solution[D]. Yangzhou: Yangzhou University, 2005.
[32]	Wang J L, Zhao X Q, Zhang H Q, Shen R F. The preference of maize plants for nitrate improves fertilizer N recovery efficiency in an acid soil partially because of alleviated Al toxicity[J]. Journal of Soils and Sediments, 2021, 21(9):3019-3033.doi: 10.1007/s11368-021-03007-9. doi: 10.1007/s11368-021-03007-9 URL
[33]	邱旭华. 水稻氮代谢基础研究:谷氨酸脱氢酶作用的分子机理[D]. 武汉: 华中农业大学, 2009.
	Qiu X H. Molecular mechanism of glutamine dehydrogenase genes for primary study of the rice nitrogen metabolite[D]. Wuhan: Huazhong Agricultural University, 2009.
[34]	莫良玉, 吴良欢, 陶勤南. 高等植物GS/GOGAT循环研究进展[J]. 植物营养与肥料学报, 2001, 7(2):223-231.doi: 10.3321/j.issn:1008-505X.2001.02.018. doi: 10.3321/j.issn:1008-505X.2001.02.018
	Mo L Y, Wu L H, Tao Q N. Research advances on GS/GOGAT cycle in higher plants[J]. Journal of Plant Nutrition and Fertilizers, 2001, 7(2):223-231.
[35]	李常健, 林清华, 张楚富. 高等植物谷氨酰胺合成酶研究进展[J]. 生物学杂志, 2001, 18(4):1-3.doi: 10.3969/j.issn.2095-1736.2001.04.001. doi: 10.3969/j.issn.2095-1736.2001.04.001
	Li C J, Lin Q H, Zhang C F. Progress of the studies on glutamine synthetase in higher plants[J]. Journal of Microbiology, 2001, 18(4):1-3.
[36]	蔡红梅. 超量表达谷氨酰胺合成酶、硝酸根转运蛋白和铵离子转运蛋白家族1基因对水稻氮代谢的功能研究[D]. 武汉: 华中农业大学, 2009.
	Cai H M. Functional analysis of overexpressing glutamine synthetase,nitrate transporter and ammonium transporter1 genes for nitrogen assimilation in rice[D]. Wuhan: Huazhong Agricultural University, 2009.
[37]	高灿红, 胡晋, 郑昀晔, 张胜. 玉米幼苗抗氧化酶活性、脯氨酸含量变化及与其耐寒性的关系[J]. 应用生态学报, 2006, 17(6):1045-1050.
	Gao C H, Hu J, Zheng Y Y, Zhang S. Antioxidant enzyme activities and proline content in maize seedling and their relationships to cold endurance[J]. Chinese Journal of Applied Ecology, 2006, 17(6):1045-1050.
[38]	吴雅薇, 李强, 豆攀, 孔凡磊, 马晓君, 程秋博, 袁继超. 低氮胁迫对不同耐低氮玉米品种苗期伤流液性状及根系活力的影响[J]. 植物营养与肥料学报, 2017, 23(2):278-288.doi: 10.11674/zwyy.16103. doi: 10.11674/zwyy.16103
	Wu Y W, Li Q, Dou P, Kong F L, Ma X J, Cheng Q B, Yuan J C. Effect of low nitrogen stress on bleeding sap characters and root activity of maize cultivars with different low N tolerance[J]. Journal of Plant Nutrition and Fertilizers, 2017, 23(2):278-288.
[39]	钟楚, 简少芬. 氮素营养调控植物逆境适应的机理研究[J]. 华北农学报, 2020, 35(S1):424-432.doi: 10.7668/hbnxb.20191237. doi: 10.7668/hbnxb.20191237
	Zhong C, Jian S F. Study on the mechanisms of nitrogen nutrition regulated plant stress-acclimation[J]. Acta Agriculturae Boreali-Sinica, 2020, 35(S1):424-432.

Please choose a citation manager

Content to export