Regulation Pattern of Photoperiod,Temperature and Abiotic Stress on SiPRR73 in Foxtail Millet

doi:10.7668/hbnxb.20193022

Abstract

Abstract:

The SiPRR73 gene was cloned from Yangu 11 using RT-PCR technology,and through analyzing tissue-specific expression,responsive features of SiPRR73 to different photoperiods,photo-thermal combinational treatments and five abiotic stress treatments,the regulation mode of photoperiod and temperature on SiPRR73,and the responsive pattern of SiPRR73 to abiotic stresses in foxtail millet were explored. The results showed that totally 2 928 bp cDNA sequence of SiPRR73 was obtained from Yangu 11,which included 2 283 bp CDS region,encoding 760 amino acids. The SiPRR73 proteins of C4 crops including Panicum miliaceum,Panicum hallii,Sorghum bicolor and Zea mays showed relatively close relationship with SiPRR73. The second parietal leaf was the highest expression tissue of SiPRR73,but the expression level at root,stem and panicle tissues was relatively lower. The expression level of SiPRR73 was higher at light period than that at dark period under both short-day and long-day conditions,and during the whole vegetative growth phase,SiPRR73 showed higher expression level under long-day compared to short-day,which indicated that the expression of SiPRR73 was induced by light and controlled by photoperiod. The temperature determined expression peak number of SiPRR73 and the photoperiod determined occurrence time of expression peaks,so temperature and photoperiod participated in regulating of SiPRR73 expression mutually. PEG and low temperature stresses induced SiPRR73 expression totally,NaCl induced SiPRR73 expression at early stress stage,but inhibited it at later stress stage. Fe stress inhibited SiPRR73 expression at early stage,but induced it at later stage. ABA stress caused the close responsive feature of SiPRR73 to NaCl. This study indicated that SiPRR73 showed light-dependent expression feature,and photoperiod and temperature regulated SiPRR73 by interaction pattern,suggesting that SiPRR73 participated in adaptability regulation process to different photo-thermal conditions and might play a certain role in coping with drought,low temperature,ABA,NaCl and Fe stresses in foxtail millet.

Key words: Foxtail millet, PRR73, Photoperiod, Temperature, Abiotic stress

YAN Liuyan, LI Jianfeng, ZHANG Shiwen, ZHANG Bo, WANG Yongfang, ZHANG Xiaomei, ZU Chaofan, WANG Zhenshan, SANG Luman, HE Zhanxiang, JIA Xiaoping, DONG Zhiping. Regulation Pattern of Photoperiod,Temperature and Abiotic Stress on SiPRR73 in Foxtail Millet[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 11-19. doi: 10.7668/hbnxb.20193022.

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Figures/Tables 8

Tab.1 Primers used for gene amplification and fluorescence quantitative PCR

引物名称 Primer name	引物序列(5'-3') Primer sequence(5'-3')	预期片段大小/bp Expected fragment size	目的 Purpose
SiPRR73-G1	F-GCTCTGCTCGTCCTCGTT	2 398	克隆SiPRR73
	R-GCCACCTCCAGCCACAT
SiPRR73-G2	F-CGGGTAACAGGGAGAGAGTAA	804
	R-CAACCACAGAAACGATAACTGG
SiActin-FQ	F-GGCAAACAGGGAGAAGATGA	229	内参基因荧光定量
	R-GAGGTTGTCGGTAAGGTCACG
SiPRR73-FQ	F-ACCATCTGTGAACTCCCTCT	198	SiPRR73荧光定量
	R-ATCTTCCTTTTCCCTCCATT

Fig.1 Total RNA and SiPRR73 gene amplified products in foxtail millet detected by electrophoresis A.Electrophoresis of total RNA of foxtail millet:1,2.Two tubes of RNA.B. Electrophoresis of SiPRR73 gene RT-PCR product:1.DL2000 Marker;2,3.Segmental amplification products,SiPRR73-G1 and SiPRR73-G2.

Fig.2 The domains contained in SiPRR73 protein

Fig.3 Molecular phylogenetic tree of PRR73 proteins

Fig.4 Expression profile of SiPRR73 in different tissue parts of foxtail millet Different lowercase letters indicate significant differences between treatments at P<0.05. The same as Fig.5-7.

Fig.5 Photoperiod responsive pattern of SiPRR73 gene A. Diurnal expression pattern;B. Expression pattern at different leaf stages.

Fig.6 The effect of photo-thermal interaction on diurnal expression pattern of SiPRR73

Fig.7 Expression pattern of SiPRR73 under abiotic stress conditions

References 47

[1]	Koo B H, Yoo S C, Park J W, Kwon C T, Lee B D, An G, Zhang Z Y, Li J J, Li Z C, Paek N C. Natural variation in OsPRR37 regulates heading date and contributes to rice cultivation at a wide range of latitudes[J]. Molecular Plant, 2013, 6(6):1877-1888. doi: 10.1093/mp/sst088. doi: 10.1093/mp/sst088 URL
[2]	李佳, 刘运华, 张余, 陈晨, 余霞, 余舜武. 干旱对水稻生物钟基因和干旱胁迫响应基因每日节律性变化的影响[J]. 遗传, 2017, 39(9):837-846. doi: 10.16288/j.yczz.17-113. doi: 10.16288/j.yczz.17-113
	Li J, Liu Y H, Zhang Y, Chen C, Yu X, Yu S W. Drought stress modulates diurnal oscillations of circadian clock and drought-responsive genes in Oryza sativa L.[J]. Hereditas, 2017, 39(9):837-846.
[3]	Campoli C, Shtaya M, Davis S J, von Korff M. Expression conservation within the circadian clock of a monocot:Natural variation at barley Ppd-H1 affects circadian expression of flowering time genes,but not clock orthologs[J]. BMC Plant Biology, 2012, 12:97. doi: 10.1186/1471-2229-12-97. doi: 10.1186/1471-2229-12-97 pmid: 22720803
[4]	Zhang W P, Zhao G Y, Gao L F, Kong X Y, Guo Z A, Wu B H, Jia J Z. Functional studies of heading date-related gene TaPRR73,a paralog of Ppd1 in common wheat[J]. Frontiers in Plant Science, 2016, 7:772. doi: 10.3389/fpls.2016.00772. doi: 10.3389/fpls.2016.00772
[5]	赵淑靓. 玉米伪应答调节基因ZmPRR73的克隆与表达分析[D]. 洛阳: 河南科技大学, 2018.
	Zhao S L. Gene cloning and ecpressing analysis of pseudo-response regulator 73(ZmPRR73)in maize[D]. Luoyang: Henan University of Science and Technology, 2018.
[6]	Brutnell T P, Wang L, Swartwood K, Goldschmidt A, Jackson D, Zhu X G, Kellogg E, van Eck J. Setaria viridis:A model for C4 photosynthesis[J]. The Plant Cell, 2010, 22(8):2537-2544. doi: 10.1105/tpc.110.075309. doi: 10.1105/tpc.110.075309 pmid: 20693355
[7]	Lata C R, Gupta S, Prasad M. Foxtail millet:A model crop for genetic and genomic studies in bioenergy grasses[J]. Critical Reviews in Biotechnology, 2013, 33(3):328-343. doi: 10.3109/07388551.2012.716809. doi: 10.3109/07388551.2012.716809 URL
[8]	贾小平, 张博, 董志平, 全建章, 王永芳, 张小梅, 袁玺垒, 李剑峰, 戴凌峰. 海南短日照条件下谷子穗部性状的全基因组关联分析[J]. 河南农业科学, 2018, 47(9):33-40. doi: 10.15933/j.cnki.1004-3268.2018.09.006. doi: 10.15933/j.cnki.1004-3268.2018.09.006
	Jia X P, Zhang B, Dong Z P, Quan J Z, Wang Y F, Zhang X M, Yuan X L, Li J F, Dai L F. Genome-wide association analysis of panicle traits of foxtail millet under Hainan short-day condition[J]. Journal of Henan Agricultural Sciences, 2018, 47(9):33-40.
[9]	贾小平, 张博, 李剑峰, 袁玺垒, 陆平, 戴凌峰. 谷子SSR标记与光周期敏感性的关联分析[J]. 华北农学报, 2019, 34(1):89-96. doi: 10.7668/hbnxb.201750672. doi: 10.7668/hbnxb.201750672
	Jia X P, Zhang B, Li J F, Yuan X L, Lu P, Dai L F. Association analysis of SSR markers and photoperiod sensitivity in foxtail millet[J]. Acta Agriculturae Boreali-Sinica, 2019, 34(1):89-96.
[10]	Makino S, Kiba T, Imamura A, Hanaki N, Nakamura A, Suzuki T, Taniguchi M, Ueguchi C, Sugiyama T, Mizuno T. Genes encoding pseudo-response regulators:Insight into his-to-asp phosphorelay and circadian rhythm in Arabidopsis thaliana[J]. Plant and Cell Physiology, 2000, 41(6):791-803. doi: 10.1093/pcp/41.6.791. doi: 10.1093/pcp/41.6.791 pmid: 10945350
[11]	李剑峰, 李婷, 贾小平. PRRs家族功能基因的研究进展[J]. 植物遗传资源学报, 2019, 20(6):1399-1407. doi: 10.13430/j.cnki.jpgr.20190403001. doi: 10.13430/j.cnki.jpgr.20190403001
	Li J F, Li T, Jia X P. Advances on unlocking the functional basis of PRRs family genes[J]. Journal of Plant Genetic Resources, 2019, 20(6):1399-1407.
[12]	Farré E M, Kay S A. PRR7 protein levels are regulated by light and the circadian clock in Arabidopsis[J]. The Plant Journal, 2007, 52(3):548-560. doi: 10.1111/j.1365-313X.2007.03258.x. doi: 10.1111/j.1365-313X.2007.03258.x URL
[13]	Matsushika A, Makino S, Kojima M, Mizuno T. Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana:Insight into the plant circadian clock[J]. Plant and Cell Physiology, 2000, 41(9):1002-1012. doi: 10.1093/pcp/pcd043. doi: 10.1093/pcp/pcd043 pmid: 11100772
[14]	Ito S, Matsushika A, Yamada H, Sato S, Kato T, Tabata S, Yamashino T, Mizuno T. Characterization of the APRR9 pseudo-response regulator belonging to the APRR1/TOC1 quintet in Arabidopsis thaliana[J]. Plant and Cell Physiology, 2003, 44(11):1237-1245. doi: 10.1093/pcp/pcg136. doi: 10.1093/pcp/pcg136 URL
[15]	Kiba T, Henriques R, Sakakibara H, Chua N H. Targeted degradation of PSEUDO-RESPONSE REGULATOR5 by an SCFZTL complex regulates clock function and photomorphogenesis in Arabidopsis thaliana[J]. The Plant Cell, 2007, 19(8):2516-2530. doi: 10.1105/tpc.107.053033. doi: 10.1105/tpc.107.053033 URL
[16]	Kaczorowski K A, Quail P H. Arabidopsis PSEUDO-RESPONSE REGULATOR7 is a signaling intermediate in phytochrome-regulated seedling deetiolation and phasing of the circadian clock[J]. The Plant Cell, 2003, 15(11):2654-2665. doi: 10.1105/tpc.015065. doi: 10.1105/tpc.015065 URL
[17]	Salomé P A, Weigel D, McClung C R. The role of the Arabidopsis morning loop components CCA1,LHY,PRR7,and PRR9 in temperature compensation[J]. The Plant Cell, 2010, 22(11):3650-3661. doi: 10.1105/tpc.110.079087. doi: 10.1105/tpc.110.079087 URL
[18]	Nakamichi N, Kiba T, Henriques R, Mizuno T, Chua N H, Sakakibara H. PSEUDO-RESPONSE REGULATORS 9,7,and 5 are transcriptional repressors in the Arabidopsis circadian clock[J]. The Plant Cell, 2010, 22(3):594-605. doi: 10.1105/tpc.109.072892. doi: 10.1105/tpc.109.072892 pmid: 20233950
[19]	Lai A G, Doherty C J, Mueller-Roeber B, Kay S A, Schippers J H, Dijkwel P P. Circadian clock-associated 1 regulates ROS homeostasis and oxidative stress responses[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(42):17129-17134. doi: 10.1073/pnas.1209148109. doi: 10.1073/pnas.1209148109
[20]	Liu T, Carlsson J, Takeuchi T, Newton L, Farré E M. Direct regulation of abiotic responses by the Arabidopsis circadian clock component PRR7[J]. The Plant Journal, 2013, 76(1):101-114. doi: 10.1111/tpj.12276. doi: 10.1111/tpj.12276
[21]	Nakamichi N, Kusano M, Fukushima A, Kita M, Ito S, Yamashino T, Saito K, Sakakibara H, Mizuno T. Transcript profiling of an Arabidopsis pseudo response regulator arrhythmic triple mutant reveals a role for the circadian clock in cold stress response[J]. Plant and Cell Physiology, 2009, 50(3):447-462. doi: 10.1093/pcp/pcp004. doi: 10.1093/pcp/pcp004 pmid: 19131357
[22]	Seo P J, Mas P. STRESSing the role of the plant circadian clock[J]. Trends in Plant Science, 2015, 20(4):230-237. doi: 10.1016/j.tplants.2015.01.001. doi: 10.1016/j.tplants.2015.01.001 URL
[23]	Keily J, MacGregor D R, Smith R W, Millar A J, Halliday K J, Penfield S. Model selection reveals control of cold signalling by evening-phased components of the plant circadian clock[J]. The Plant Journal, 2013, 76(2):247-257. doi: 10.1111/tpj.12303. doi: 10.1111/tpj.12303 pmid: 23909712
[24]	Briat J F, Ravet K, Arnaud N, Duc C, Boucherez J, Touraine B, Cellier F, Gaymard F. New insights into ferritin synthesis and function highlight a link between iron homeostasis and oxidative stress in plants[J]. Annals of Botany, 2010, 105(5):811-822. doi: 10.1093/aob/mcp128. doi: 10.1093/aob/mcp128 URL
[25]	贾小平, 李剑峰, 全建章, 王永芳, 董志平, 张博, 袁玺垒. 不同光周期条件下谷子农艺性状的光周期敏感性评价[J]. 植物遗传资源学报, 2018, 19(5):919-924. doi: 10.13430/j.cnki.jpgr.20180207005. doi: 10.13430/j.cnki.jpgr.20180207005
	Jia X P, Li J F, Quan J Z, Wang Y F, Dong Z P, Zhang B, Yuan X L. Evaluation of photoperiod sensitivity of agronomic traits of foxtail millet varieties(Setaria italica)under different photoperiod conditions[J]. Journal of Plant Genetic Resources, 2018, 19(5):919-924.
[26]	贾小平, 袁玺垒, 李剑峰, 张博, 张小梅, 郭秀璞, 陈春燕. 不同光温条件谷子资源主要农艺性状的综合评价[J]. 中国农业科学, 2018, 51(13):2429-2441. doi: 10.3864/j.issn.0578-1752.2018.13.001. doi: 10.3864/j.issn.0578-1752.2018.13.001
	Jia X P, Yuan X L, Li J F, Zhang B, Zhang X M, Guo X P, Chen C Y. Comprehensive evaluation of main agronomic traits of millet resources under different light and temperature conditions[J]. Scientia Agricultura Sinica, 2018, 51(13):2429-2441.
[27]	袁玺垒, 戴凌峰, 张小梅, 郁飞燕, 贾小平. 谷子Hd1-like基因的克隆及其与农艺性状的关联分析[J]. 河南农业科学, 2018, 47(6):24-30. doi: 10.15933/j.cnki.1004-3268.2018.06.005. doi: 10.15933/j.cnki.1004-3268.2018.06.005
	Yuan X L, Dai L F, Zhang X M, Yu F Y, Jia X P. Putative Hd1-like gene cloning and its preliminary association with agronomic traits in foxtail millet(Setaria italica)[J]. Journal of Henan Agricultural Sciences, 2018, 47(6):24-30.
[28]	Ni X M, Xia Q J, Zhang H B, Cheng S, Li H, Fan G Y, Guo T, Huang P, Xiang H T, Chen Q C, Li N, Zou H F, Cai X M, Lei X J, Wang X M, Zhou C S, Zhao Z H, Zhang G Y, Du G H, Cai W, Quan Z W. Updated foxtail millet genome assembly and gene mapping of nine key agronomic traits by resequencing a RIL population[J]. GigaScience, 2017, 6(2):giw005. doi: 10.1093/gigascience/giw005. doi: 10.1093/gigascience/giw005
[29]	贾小平, 张博, 全建章, 李剑峰, 王永芳, 袁玺垒. 不同光周期条件下谷子株高的全基因组关联分析[J]. 华北农学报, 2019, 34(4):16-23. doi: 10.7668/hbnxb.201750856. doi: 10.7668/hbnxb.201750856
	Jia X P, Zhang B, Quan J Z, Li J F, Wang Y F, Yuan X L. Genome-wide association analysis of plant height in foxtail millet under different photoperiod conditions[J]. Acta Agriculturae Boreali-Sinica, 2019, 34(4):16-23.
[30]	Doust A N, Mauro-Herrera M, Hodge J G, Stromski J. The C4 model grass Setaria is a short day plant with secondary long day genetic regulation[J]. Frontiers in Plant Science, 2017, 8:1062. doi: 10.3389/fpls.2017.01062. doi: 10.3389/fpls.2017.01062 URL
[31]	谢丽莉. 谷子光周期敏感相关性状的QTL定位与分析[D]. 郑州: 河南农业大学, 2012.
	Xie L L. OTL mapping and analysis for the related traits of photoperiod sensitivity in Setaria italica[D]. Zhengzhou: Henan Agricultural University, 2012.
[32]	Jia G Q, Huang X H, Zhi H, Zhao Y, Zhao Q, Li W J, Chai Y, Yang L F, Liu K Y, Lu H Y, Zhu C R, Lu Y Q, Zhou C C, Fan D L, Weng Q J, Guo Y L, Huang T, Zhang L, Lu T T, Feng Q, Hao H F, Liu H K, Lu P, Zhang N, Li Y H, Guo E H, Wang S J, Wang S Y, Liu J R, Zhang W F, Chen G Q, Zhang B J, Li W, Wang Y F, Li H Q, Zhao B H, Li J Y, Diao X M, Han B. A haplotype map of genomic variations and genome-wide association studies of agronomic traits in foxtail millet(Setaria italica)[J]. Nature Genetics, 2013, 45(8):957-961. doi: 10.1038/ng.2673. doi: 10.1038/ng.2673 URL
[33]	Zhang K, Fan G Y, Zhang X X, Zhao F, Wei W, Du G H, Feng X L, Wang X M, Wang F, Song G L, Zou H F, Zhang X L, Li S D, Ni X M, Zhang G Y, Zhao Z H. Identification of QTLs for 14 agronomically important traits in Setaria italica based on SNPs generated from high-throughput sequencing[J]. G3 Genes\|Genomes\|Genetics, 2017, 7(5):1587-1594. doi: 10.1534/g3.117.041517. doi: 10.1534/g3.117.041517
[34]	贾小平, 袁玺垒, 李剑峰, 王永芳, 张小梅, 张博, 全建章, 董志平. 不同光温条件谷子光温互作模式研究及SiCCT基因表达分析[J]. 作物学报, 2020, 46(7):1052-1062. doi: 10.3724/SP.J.1006.2020.94144. doi: 10.3724/SP.J.1006.2020.94144
	Jia X P, Yuan X L, Li J F, Wang Y F, Zhang X M, Zhang B, Quan J Z, Dong Z P. Photo-thermal interaction model under different photoperiod-temperature conditions and expression analysis of SiCCT gene in foxtail millet(Setaria italica L.)[J]. Acta Agronomica Sinica, 2020, 46(7):1052-1062. doi: 10.3724/SP.J.1006.2020.94144 URL
[35]	陈华夏, 申国境, 王磊, 邢永忠. 4个物种CCT结构域基因家族的序列进化分析[J]. 华中农业大学学报, 2010, 29(6):669-676. doi: 10.13300/j.cnki.hnlkxb.2010.06.001. doi: 10.13300/j.cnki.hnlkxb.2010.06.001
	Chen H X, Shen G J, Wang L, Xing Y Z. Sequence evolution analysis of CCT domain gene family in rice,Arabidopsis,maize and Sorghum[J]. Journal of Huazhong Agricultural University, 2010, 29(6):669-676.
[36]	Zhang L, Li Q P, Dong H J, He Q, Liang L W, Tan C, Han Z M, Yao W, Li G W, Zhao H, Xie W B, Xing Y Z. Three CCT domain-containing genes were identified to regulate heading date by candidate gene-based association mapping and transformation in rice[J]. Scientific Reports, 2015, 5:7663. doi: 10.1038/srep07663. doi: 10.1038/srep07663 pmid: 25563494
[37]	Liu C, Song G Y, Zhou Y H, Qu X F, Guo Z B, Liu Z W, Jiang D M, Yang D C. OsPRR37 and Ghd7 are the major genes for general combining ability of DTH,PH and SPP in rice[J]. Scientific Reports, 2015, 5:12803. doi: 10.1038/srep12803. doi: 10.1038/srep12803 URL
[38]	Balasubramanian S, Sureshkumar S, Lempe J, Weigel D. Potent induction of Arabidopsis thaliana flowering by elevated growth temperature[J]. PLoS Genetics, 2006, 2(7):e106. doi: 10.1371/journal.pgen.0020106. doi: 10.1371/journal.pgen.0020106 pmid: 16839183
[39]	Hemming M N, Walford S A, Fieg S, Dennis E S, Trevaskis B. Identification of high-temperature-responsive genes in cereals[J]. Plant Physiology, 2012, 158(3):1439-1450. doi: 10.1104/pp.111.192013. doi: 10.1104/pp.111.192013 pmid: 22279145
[40]	Cober E R, Stewart D W, Voldeng H D. Crop physiology & metabolism:Photoperiod and temperature responses in early-maturing,near-isogenic soybean lines[J]. Crop Science, 2001, 41:721-727. doi: 10.2135/cropsci2001. 413721x. doi: 10.2135/cropsci2001. 413721x URL
[41]	刘易科. 大豆光温互作新模型的验证和FT家族基因的克隆[D]. 杨凌: 西北农林科技大学, 2006.
	Liu Y K. Testify a new soybean model of photo-thermal interaction and clone FT family genes[D]. Yangling: Northwest A&F University, 2006.
[42]	孙洪波. 大豆光温互作新模式的验证及PEBP家族基因的克隆和功能分析[D]. 北京: 中国农业科学院, 2008.
	Sun H B. Validation of a new soybean light-temperature interaction model and cloning and functional analysis of PEBP family genes[D]. Beijing: Chinese Academy of Agricultural Sciences, 2008.
[43]	Grundy J, Stoker C, Carré I A. Circadian regulation of abiotic stress tolerance in plants[J]. Frontiers in Plant Science, 2015, 6:648. doi: 10.3389/fpls.2015.00648. doi: 10.3389/fpls.2015.00648 pmid: 26379680
[44]	Shrestha R, Gómez-Ariza J, Brambilla V, Fornara F. Molecular control of seasonal flowering in rice,Arabidopsis and temperate cereals[J]. Annals of Botany, 2014, 114(7):1445-1458. doi: 10.1093/aob/mcu032. doi: 10.1093/aob/mcu032 pmid: 24651369
[45]	Lister D L, Thaw S, Bower M A, Jones H, Charles M P, Jones G, Smith L M J, Howe C J, Brown T A, Jones M K. Latitudinal variation in a p.hotoperiod response gene in European barley:insight into the dynamics of agricultural spread from Historic specimens[J]. Journal of Archaeological Science, 2009, 36:1092-1098. doi: 10.1016/j. jas.2008.12.012. doi: 10.1016/j. jas.2008.12.012 URL
[46]	Kurup S, Jones H D, Holdsworth M J. Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds[J]. The Plant Journal, 2000, 21(2):143-155. doi: 10.1046/j.1365-313x.2000.00663.x. doi: 10.1046/j.1365-313x.2000.00663.x URL
[47]	向芬, 黄帅, 赵小英, 刘选明, 朱咏华. 拟南芥prr5突变体对ABA的响应[J]. 激光生物学报, 2013, 22(6):546-550. doi: 10.3969/j.issn.1007-7146.2013.06.012. doi: 10.3969/j.issn.1007-7146.2013.06.012
	Xiang F, Huang S, Zhao X Y, Liu X M, Zhu Y H. The response of the prr5 mutant to ABA in Arabidopsis thaliana[J]. Acta Laser Biology Sinica, 2013, 22(6):546-550.

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