Physiological Response Mechanism of Rye to Low Temperature During Cold Hardening and Rejuvenation Period

doi:10.7668/hbnxb.20194655

Abstract

Abstract:

In order to reveal the physiological mechanism of winter rye in response to low temperature stress,the physiological changes of osmoregulatory substances and antioxidant enzyme activity in the leaves and tillering nodes of Wintergraze 70 and White BK-1 were compared during the cold hardening and rejuvenation periods.The results showed that during the cold hardening period,rye mainly improved its cold resistance by accumulating osmoregulatory substances in leaves and tillering nodes.During this period,the semi lethal temperature gradually decreased,and the semi lethal temperature of White BK-1 reached -9.93 ℃,which was 1.95 ℃ lower than Wintergraze 70 while the soil freezing.The increase of soluble sugar and soluble protein contents in the leaves and tillering nodes of White BK-1 was greater than those of Wintergraze 70,which meant these physiological pathways played an important role in improving the tolerance of White BK-1 to low temperatures in winter.Research on the rejuvenation period found that with the extension of low temperature stress time,the content of malondialdehyde first increased and then decreased.Rye resisted the low temperature of rejuvenation period by increasing the content of osmoregulatory substances,improving the activity of antioxidant enzymes in leaves and regulating the activity of antioxidant enzymes in tillering nodes.White BK-1 suffered less damage under the low temperature stress during rejuvenation period,and its malondialdehyde content was lower than Wintergraze 70 after recovery.In addition,after recovery,two varieties of rye accumulated higher contents of proline and soluble protein in the tillering nodes,which providing sufficient nutrients for growth and development after rejuvenation.

Key words: Rye, Cold hardening, Rejuvenation, Low temperature physiology

YANG Chaowei, SUN Weihong, REN Wei, WANG Dan, AN Mingzhu, GENG Feilong, WANG Xianguo. Physiological Response Mechanism of Rye to Low Temperature During Cold Hardening and Rejuvenation Period[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(3): 104-113. doi: 10.7668/hbnxb.20194655.

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References 36

[1]	刘爱民, 贾盼娜, 王立新, 吴良杰. 我国饲(草)料供求及未来需求预测和对策研究[J]. 中国工程科学, 2018, 20(5):39-44.doi:10.15302/J-SSCAE-2018.05.006.
	Liu A M, Jia P N, Wang L X, Wu L J. Supply and demand analysis,future demand forecast,and countermeasure studies for forage materials in China[J]. Strategic Study of CAE, 2018, 20(5):39-44.
[2]	李洪泉, 高兰阳, 刘刚, 李华德. 草畜优化条件下草地生态载畜量测算方法新探[J]. 草业学报, 2009, 18(5):262-265.doi:10.3321/j.issn:1004-5759.2009.05.034.
	Li H Q, Gao L Y, Liu G, Li H D. A new probe in to the method of calculating pasture ecological livestock capability on the grass-livestock optimize[J]. Acta Prataculturae Sinica, 2009, 18(5):262-265.
[3]	王伟强, 田新会, 杜文华. 甘农1号黑麦在青藏高原高寒牧区的生产性能研究[J]. 草原与草坪, 2021, 41(3):57-62.doi:10.13817/j.cnki.cyycp.2021.03.008.
	Wang W Q, Tian X H, Du W H. Forage productivity of Secale cereal cv.Gannong No.1 in Qinghai-Tibet alpine pastoral area[J]. Grassland and Turf, 2021, 41(3):57-62.
[4]	Fuller M P, Fuller A M, Kaniouras S, Christophers J, Fredericks T. The freezing characteristics of wheat at ear emergence[J]. European Journal of Agronomy, 2007, 26(4):435-441.doi:10.1016/j.eja.2007.01.001.
[5]	Ensminger I, Busch F, Huner N P A. Photostasis and cold acclimation:sensing low temperature through photosynthesis[J]. Physiologia Plantarum, 2006, 126(1):28-44.doi:10.1111/j.1399-3054.2006.00627.x.
[6]	Livingston D P, Premakumar R, Tallury S P. Carbohydrate partitioning between upper and lower regions of the crown in oat and rye during cold acclimation and freezing[J]. Cryobiology, 2006, 52(2):200-208.doi:10.1016/j.cryobiol.2005.11.001. pmid: 16359655
[7]	Janmohammadi M, Sabaghnia N, Mahfoozi S. Frost tolerance and metabolite changes of rye(Secale cereale)during the cold hardening and overwintering[J]. Acta Physiologiae Plantarum, 2018, 40(3):42.doi:10.1007/s11738-018-2620-0.
[8]	Yu X R, Jiang Y F, Yao H H, Ran L P, Zang Y, Xiong F. Cytological and molecular characteristics of delayed spike development in wheat under low temperature in early spring[J]. The Crop Journal, 2022, 10(3):840-852.doi:10.1016/j.cj.2021.08.008.
[9]	Popov V N, Naraikina N V. Change of antioxidant enzyme activity during low-temperature hardening of Nicotiana tabacum L.and Secale cereale L.[J]. Russian Journal of Plant Physiology, 2020, 67(5):898-905.doi:10.1134/S1021443720050118.
[10]	刘杰. 低温干旱复合胁迫对黑麦、小黑麦、短芒披碱草幼苗的抗逆性研究[D]. 拉萨: 西藏大学, 2017.doi:10.7666/d.D01204759.
	Liu J. Study on stress resistance of rye,triticale and Elymus breviscapus seedlings under combined stress of low temperature and drought[D]. Lasa: Tibet University, 2017.
[11]	张舒芸. 小黑麦和黑麦的抗旱性与抗寒性研究[D]. 兰州: 甘肃农业大学, 2018.
	Zhang S Y. Studies on the drought and cold resistance of triticale and rye[D]. Lanzhou: Gansu Agricultural University, 2018.
[12]	Bahrani H, Thoms K, Båga M, Larsen J, Graf R, Laroche A, Sammynaiken R, Chibbar R N. Preferential accumulation of glycosylated cyanidins in winter-hardy rye(Secale cereale L.)genotypes during cold acclimation[J]. Environmental and Experimental Botany, 2019, 164:203-212.doi:10.1016/j.envexpbot.2019.05.006.
[13]	艾雅雯, 孙建奇, 韩双泽, 张梦琪, 华维. 1961—2016年中国春季极端低温事件的时空特征分析[J]. 大气科学, 2020, 44(6):1305-1319.doi:10.3878/j.issn.1006-9895.1912.19223.
	Ai Y W, Sun J Q, Han S Z, Zhang M Q, Hua W. Spatial and temporal features of spring extreme low temperature events in China during 1961—2016[J]. Chinese Journal of Atmospheric Sciences, 2020, 44(6):1305-1319.
[14]	赵瑞玲, 赵勇, 徐渴, 李嘉豪, 张树华, 杨学举. 室内冷冻法鉴定小麦抗寒性的研究[J]. 植物遗传资源学报, 2019, 20(2):284-296.doi:10.13430/j.cnki.jpgr.20180719001.
	Zhao R L, Zhao Y, Xu K, Li J H, Zhang S H, Yang X J. Identification of cold resistance wheat varieties by laboratory freezing method[J]. Journal of Plant Genetic Resources, 2019, 20(2):284-296.
[15]	张志良, 瞿伟菁, 李小方. 植物生理学实验指导[M]. 4版. 北京: 高等教育出版社, 2009.
	Zhang Z L, Qu W J, Li X F. Guidance on plant physiology experiments[M]. 4th Edition.Beijing: Higher Education Press, 2009.
[16]	衣莹, 张玉龙, 郭志富, 白丽萍, 乔雪, 侯立白. 冬小麦叶片对低温胁迫的生理响应[J]. 华北农学报, 2013, 28(1):144-148.doi:10.3969/j.issn.1000-7091.2013.01.027.
	Yi Y, Zhang Y L, Guo Z F, Bai L P, Qiao X, Hou L B. Physiological responses of winter wheat's leaves to low temperature stress[J]. Acta Agriculturae Boreali-Sinica, 2013, 28(1):144-148. doi: 10.3969/j.issn.1000-7091.2013.01.027
[17]	陈禹兴, 付连双, 王晓楠, 李卓夫. 低温胁迫对冬小麦恢复生长后植株细胞膜透性和丙二醛含量的影响[J]. 东北农业大学学报, 2010, 41(10):10-16.doi:10.19720/j.cnki.issn.1005-9369.2010.10.003.
	Chen Y X, Fu L S, Wang X N, Li Z F. Effect of freezing stress on membrane permeability and MDA content in the re-growth plant of winter wheat cultivars[J]. Journal of Northeast Agricultural University, 2010, 41(10):10-16.
[18]	吴宇欣, 蔡昌杨, 唐诗蓓, 谢裕红, 王晓艳, 朱强. 植物响应低温的生长发育及分子机制研究进展[J]. 江苏农业科学, 2023, 51(19):1-9.doi:10.15889/j.issn.1002-1302.2023.19.001.
	Wu Y X, Cai C Y, Tang S B, Xie Y H, Wang X Y, Zhu Q. Research progress on plant growth and molecular mechanism in response to low temperature[J]. Jiangsu Agricultural Sciences, 2023, 51(19):1-9.
[19]	苏李维, 李胜, 马绍英, 戴彩虹, 时振振, 唐斌, 赵生琴, 蒲彦涛. 葡萄抗寒性综合评价方法的建立[J]. 草业学报, 2015, 24(3):70-79.doi:10.11686/cyxb20150307.
	Su L W, Li S, Ma S Y, Dai C H, Shi Z Z, Tang B, Zhao S Q, Pu Y T. A comprehensive assessment method for cold resistance of grape vines[J]. Acta Prataculturae Sinica, 2015, 24(3):70-79. doi: 10.11686/cyxb20150307
[20]	丁美云, 刘丽杰, 于梦迪, 王璐瑶, 孙玉婷, 金忠民, 李珊珊, 张东向. 油菜素内酯对寒地冬小麦越冬期生理代谢的影响[J]. 麦类作物学报, 2022, 42(7):864-871.doi:10.7606/j.issn.1009-1041.2022.07.10.
	Ding M Y, Liu L J, Yu M D, Wang L Y, Sun Y T, Jin Z M, Li S S, Zhang D X. Effect of brassinolide on physiological metabolism of winter wheat in cold area[J]. Journal of Triticeae Crops, 2022, 42(7):864-871.
[21]	曹燕燕, 张宏套, 郭春强, 葛昌斌, 廖平安, 黄杰, 乔冀良, 齐双丽, 李雷雷. 不同冬小麦品种拔节期低温生理生化反应及其灰色关联度分析[J]. 山东农业科学, 2021, 53(8):37-42.doi:10.14083/j.issn.1001-4942.2021.08.007.
	Cao Y Y, Zhang H T, Guo C Q, Ge C B, Liao P A, Huang J, Qiao J L, Qi S L, Li L L. Physiological and biochemical reactions of different winter wheat varieties at jointing stage under low temperature and their grey correlation analysis[J]. Shandong Agricultural Sciences, 2021, 53(8):37-42.
[22]	陈翔, 林涛, 林非非, 张妍, 苏慧, 胡燕美, 宋有洪, 魏凤珍, 李金才. 黄淮麦区小麦倒春寒危害机理及防控措施研究进展[J]. 麦类作物学报, 2020, 40(2):243-250.doi:10.7606/j.issn.1009-1041.2020.02.14.
	Chen X, Lin T, Lin F F, Zhang Y, Su H, Hu Y M, Song Y H, Wei F Z, Li J C. Research progress on damage mechanism and prevention and control measures of late spring coldness of wheat in Huanghuai Region[J]. Journal of Triticeae Crops, 2020, 40(2):243-250.
[23]	陈思琪, 孙敬爽, 麻文俊, 王军辉, 赵曦阳, 胡瑞阳. 植物低温胁迫调控机制研究进展[J]. 中国农学通报, 2022, 38(17):51-61.doi:10.11924/j.issn.1000-6850.cabs-2021-0656.
	Chen S Q, Sun J S, Ma W J, Wang J H, Zhao X Y, Hu R Y. Regulation mechanism of low temperature stress on plants:research progress[J]. Chinese Agricultural Science Bulletin, 2022, 38(17):51-61.
[24]	王金伟, 刘灿, 李卓夫, 付连双, 孙艳丽, 王晓楠, 陈禹兴. 低温驯化期冬小麦生理指标变化的比较[J]. 东北农业大学学报, 2009, 40(9):1-5.doi:10.19720/j.cnki.issn.1005-9369.2009.09.001.
	Wang J W, Liu C, Li Z F, Fu L S, Sun Y L, Wang X N, Chen Y X. Comparison of physiological changes of winter wheat during cold acclimation period[J]. Journal of Northeast Agricultural University, 2009, 40(9):1-5.
[25]	张自阳, 王智煜, 王斌, 王志伟, 朱启迪, 霍云风, 茹振钢, 刘明久. 春季穗分化阶段低温处理对不同小麦品种幼穗结实性及生理特性的影响[J]. 华北农学报, 2019, 34(4):130-139.doi:10.7668/hbnxb.20190321.
	Zhang Z Y, Wang Z Y, Wang B, Wang Z W, Zhu Q D, Huo Y F, Ru Z G, Liu M J. Effects of low temperature treatment at spring spike differentiation stage on young ear fruiting and physiological characteristics of different wheat varieties[J]. Acta Agriculturae Boreali-Sinica, 2019, 34(4):130-139. doi: 10.7668/hbnxb.20190321
[26]	张志伟, 王法宏, 李升东, 冯波, 司纪升, 张宾, 孔令安, 王峥. 不同类型小麦品种孕穗期低温生理反应及其抗寒性分析[J]. 麦类作物学报, 2012, 32(5):900-906.
	Zhang Z W, Wang F H, Li S D, Feng B, Si J S, Zhang B, Kong L A, Wang Z. Analysis on the physiological reaction to low temperature at booting stage and their cold resistance of different types of wheat varieties[J]. Journal of Triticeae Crops, 2012, 32(5):900-906.
[27]	Burnett A C, Kromdijk J. Can we improve the chilling tolerance of maize photosynthesis through breeding?[J]. Journal of Experimental Botany, 2022, 73(10):3138-3156.doi:10.1093/jxb/erac045. pmid: 35143635
[28]	李鹏, 马骊, 徐芳, 刘丽君, 姚彦林, 蒲媛媛, 王旺田, 李学才, 方彦, 孙万仓, 武军艳. 越冬前北方不同类型强冬性冬油菜形态及生理响应研究[J]. 干旱地区农业研究, 2022, 40(5):42-51.doi:10.7606/j.issn.1000-7601.2022.05.05.
	Li P, Ma L, Xu F, Liu L J, Yao Y L, Pu Y Y, Wang W T, Li X C, Fang Y, Sun W C, Wu J Y. Comparison of morphological and physiological responses of two different species of northern winter rapeseed before overwintering[J]. Agricultural Research in the Arid Areas, 2022, 40(5):42-51.
[29]	Bao Y Z, Xing J P, Liang Y, Ren Z P, Fu L S, Yu J, Wang D J, Zhang D, Xu Q H, Cang J. Analysis of overwintering indexes of winter wheat in alpine regions and establishment of a cold resistance model[J]. Field Crops Research, 2022, 275:108347.doi:10.1016/j.fcr.2021.108347.
[30]	李莉莎, 徐海燕, 吴晓东, 毛楠, 甘子鹏, 薛守业. 青藏高原高山嵩草叶、根抗寒性生理特征[J]. 草地学报, 2020, 28(6):1544-1551.doi:10.11733/j.issn.1007-0435.2020.06.007.
	Li L S, Xu H Y, Wu X D, Mao N, Gan Z P, Xue S Y. Cold resistance physiology characteristics of leaf and root of Kobresia pygmaea on the Tibetan Plateau[J]. Acta Agrestia Sinica, 2020, 28(6):1544-1551. doi: 10.11733/j.issn.1007-0435.2020.06.007
[31]	王小华, 庄南生. 脯氨酸与植物抗寒性的研究进展[J]. 中国农学通报, 2008, 24(11):398-402.
	Wang X H, Zhuang N S. Advances in research on proline and cold resistance of plant[J]. Chinese Agricultural Science Bulletin, 2008, 24(11):398-402.
[32]	李轶冰, 杨顺强, 任广鑫, 冯永忠, 张强, 李鹏. 低温处理下不同禾本科牧草的生理变化及其抗寒性比较[J]. 生态学报, 2009, 29(3):1341-1347.doi:10.3321/j.issn:1000-0933.2009.03.030.
	Li Y B, Yang S Q, Ren G X, Feng Y Z, Zhang Q, Li P. Changes analysis in physiological properties of several gramineous grass species and cold-resistance comparison on under cold stress[J]. Acta Ecologica Sinica, 2009, 29(3):1341-1347.
[33]	衣莹, 张玉龙, 郭志富, 白丽萍, 侯立白. 越冬及返青期冬小麦生理生化指标的变化[J]. 江苏农业科学, 2013, 41(1):81-83.doi:10.15889/j.issn.1002-1302.2013.01.099.
	Yi Y, Zhang Y L, Guo Z F, Bai L P, Hou L B. Changes of physiological and biochemical indexes of winter wheat during overwintering and greening period[J]. Jiangsu Agricultural Sciences, 2013, 41(1):81-83.
[34]	徐静, 唐运来, 王建宝, 王丹. Cs对菠菜叶片光合作用影响的研究[J]. 核农学报, 2015, 29(5):986-994.doi:10.11869/j.issn.100-8551.2015.05.0986.
	Xu J, Tang Y L, Wang J B, Wang D. Study on the effects of cesium on photosynthesis of spinach[J]. Journal of Nuclear Agricultural Sciences, 2015, 29(5):986-994. doi: 10.11869/j.issn.100-8551.2015.05.0986
[35]	曹燕燕, 葛昌斌, 齐双丽, 廖平安, 黄杰, 王君, 李雷雷, 乔冀良, 张振永. 不同冬小麦品种(系)拔节期低温胁迫生理反应及抗寒性评价[J]. 江苏农业科学, 2022, 50(17):59-66.doi:10.15889/j.issn.1002-1302.2022.17.010.
	Cao Y Y, Ge C B, Qi S L, Liao P A, Huang J, Wang J, Li L L, Qiao J L, Zhang Z Y. Physiological response to low temperature stress and evaluation of cold resistance of different winter wheat varieties(lines)at jointing stage[J]. Jiangsu Agricultural Sciences, 2022, 50(17):59-66.
[36]	孙苗苗, 王志强, 高翔, 龚璞, 辛泽毓, 林同保. 河南主推小麦品种对低温胁迫的生理响应及耐寒性分析[J]. 麦类作物学报, 2016, 36(3):316-324.doi:10.7606/j.issn.1009-1041.2016.03.09.
	Sun M M, Wang Z Q, Gao X, Gong P, Xin Z Y, Lin T B. Cold tolerance evaluation of wheat varieties in Henan based on their physiological response to low temperature stress[J]. Journal of Triticeae Crops, 2016, 36(3):316-324.

日期/(月-日) Date	最高气温 Maximum temperature	最低气温 Minimum temperature	平均气温 Average temperature
10-31	18.0	2.0	10.0
11-10	13.0	0.0	6.5
11-20	14.0	4.0	9.0
12-10	3.0	-6.0	-1.5
12-20	0.0	-8.0	-4.0

日期/(月-日) Date	最高气温 Maximum temperature	最低气温 Minimum temperature	平均气温 Average temperature
10-31	18.0	2.0	10.0
11-10	13.0	0.0	6.5
11-20	14.0	4.0	9.0
12-10	3.0	-6.0	-1.5
12-20	0.0	-8.0	-4.0

日期/(月-日) Date	冬牧70 Wintergraze 70	白BK-1 White BK-1
10-31	0.617±0.050ab	0.657±0.056ab
11-10	0.592±0.075b	0.685±0.072a
11-20	0.633±0.082ab	0.683±0.052a
12-10	0.610±0.085b	0.677±0.035a
12-20	0.601±0.056b	0.647±0.032ab

日期/(月-日) Date	冬牧70 Wintergraze 70	白BK-1 White BK-1
10-31	0.617±0.050ab	0.657±0.056ab
11-10	0.592±0.075b	0.685±0.072a
11-20	0.633±0.082ab	0.683±0.052a
12-10	0.610±0.085b	0.677±0.035a
12-20	0.601±0.056b	0.647±0.032ab

日期/(月-日) Date	冬牧70 Wintergraze 70	白BK-1 White BK-1
10-31	-3.84±0.44a	-3.89±0.87a
11-10	-5.35±1.76b	-5.61±0.42b
11-20	-5.94±0.57b	-6.26±0.86b
12-10	-7.59±0.39c	-8.73±0.52c
12-20	-7.98±0.70c	-9.93±0.60d

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