Improvement of Perennial Ryegrass (Lolium perenne) Tolerance to Drought by Gene Transferring of Stress-inducible Transcription Factor CBF1 Gene

doi:10.3321/j.issn:1000-7091.2006.01.004

Abstract

Abstract: In this research,two expression vectors pBAC122 and pBAC127 containing CBF1 gene were constructed, gene is driven by rd29B promoter,and bar gene driven by CaMV35S promoter as selection marker.By biolistic bombardment of PDS1000/He system,both expression vectors(pBAC₁22 was co-transferred with p35SIH3) were transformed into callus,immature embryos and mature embryos of perennerial ryegrass(Lolium perenne),respectively.After screening by Bialaphos and plant regeneration culture,36 transgenic plants were produced.The results of PCR and Dot-blotting analysis showed that foreign gene were well integrated into some transgenic plant's genome of ryegrass,respectively.Treatment of transgenic plants under water stress or 15%PEG,the free proline content of transgenic plants increase markedly,the increased extent was obviously higher than non-transgenic wild type.After 25 days of drought stress,3 transgenic lines behave green and living,and 1 transgenic lines restored living after watering.Our research suggested that using drought-inducible rd29B gene promoter to enhance the expression of foreign gene in ryegrass can greatly improve its tolerance to drought.

Key words: Perennial ryegrass, Transgenic, Free proline content, Tolerance to drought

CLC Number:

S544

YANG Feng-ping, LIANG Rong-qi, ZHANG Li-quan, CHEN Xu-qing, ZHANG Xiao-dong, WANG Guo-ying, SUN Zhen-yuan. Improvement of Perennial Ryegrass (Lolium perenne) Tolerance to Drought by Gene Transferring of Stress-inducible Transcription Factor CBF₁ Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 14-18. doi: 10.3321/j.issn:1000-7091.2006.01.004.

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References

[1] 粱慧敏,夏阳,杜蜂,等.盐胁迫对两种草坪草抗性生理生化指标影响的研究[J].中国草地,2001,23(5):27-30.
[2] 刘强,张贵友,陈受宜.植物转录因子的结构与调控作用[J].科学通报,2000,45(7):1465-1474.
[3] Eric J,Mao Y,Rejier M K,et al.Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF₁,a transcriptional activator involved in cold-regulated gene expression[J].Nucleic Acids Research,2001,29:1524-1533.
[4] Haake V,Cook D,Riechmann J L,et al.Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis[J].Plant Physiology,2002,130:639-648.
[5] Foolad M R,Zhang L P,Lin G Y.Identification and validation of QTLs for salt tolerance during vegetative growth in tomato by selective genotyping[J].Genome,2001,44:444-454.
[6] 刘强,赵南明,Yamaguch-ShinozakiK,等.DREB转录因子在提高植物抗逆中的作用[J].科学通报,2000,45(1):11-16.
[7] 张晓东,梁荣奇,陈绪清,等.优质HMW谷蛋白亚基转基因小麦的获得及其遗传稳定性和品质性状分析[J].科学通报,2003,48(5):474-479.
[8] 徐晓峰,朱才.小麦叶中脯氨酸测定方法的研究[J].生物技术,1997,7(1):40-42.
[9] Kasug M,Liu Q,Miura S,et al.Improving plant drought,salt,and freezing tolerance by gene transfer of a single stressinducible transcription factor [J].Nature Biotechnology,1999,17:287-292.
[10] Liu Q,Kasuga M,Sakuma Y,et al.Two transcription factors,DREB1 and DREB2,with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression,respectively,in Arabidopsis[J].The Plant Cell,1998,10:1391-1406.
[11] 刘宁,高玉葆,贾彩霞,等.渗透胁迫下多花黑麦草叶内过氧化物酶活性和脯氨酸含量以及质膜相对透性的变化[J].植物生理学通讯,2000,36(1):11-14.
[12] 高玉葆,任安芝,刘峰,等.黑麦草叶内游离脯氨酸含量对于不同类型和强度的水分胁迫的生理生态响应[J].植物生理学通讯,1999,23(3):193-204.
[13] 高玉葆,任安芝,刘蜂.模拟草地内黑麦草叶游离脯氨酸含量与叶含水量、土壤含水量之间的相互关系研究[J].南开大学学报(自然科学版),1999,32(3):169-176.
[14] 苏金,朱汝财.渗透胁迫调节的转基因表达对植物抗旱耐盐性的影响[J].植物学通报,2001,18(2):129-136.

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Improvement of Perennial Ryegrass (Lolium perenne) Tolerance to Drought by Gene Transferring of Stress-inducible Transcription Factor CBF₁ Gene

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