Construction of Overexpression Vector of Maize ZmMGT10 Gene and Transformation of Arabidopsis thaliana

doi:10.7668/hbnxb.2017.06.001

Abstract

Abstract: CorA/MRS2/MGT-type magnesium ion transporters play an important role in maintaining magnesium homeostasis in plants.In order to investigate the function of ZmMGT10 gene which is a maize CorA/MRS2/MGT-type magnesium ion transporter and expression is induced by magnesium deficiency,we constructed the overexpression vector of ZmMGT10 and transgenic Arabidopsis plants were obtained.Compared with wild-type plants,transgenic Arabidopsis plants grew more vigorously than wild-type plants under low Mg conditions,exhibited by longer root length,higher plant fresh weight and chlorophyll content.Further analysis indicated that the roots and shoots of transgenic plants had higher magnesium accumulation than wild-type plants under low magnesium condition.Furthermore,the roots of transgenic plants had enhanced magnesium uptake ability than wild-type plants.The results suggested that overexpression ZmMGT10 in Arabidopsis could enhance the resistance ability of transgenic plants to magnesium deficiency stress.

Key words: Maize, ZmMGT10, Magnesium ion transporter, Arabidopsis, Transgenic plants

CLC Number:

Q78
S513.03

LI Hongyou, ZHANG Suzhi, CHEN Qingfu. Construction of Overexpression Vector of Maize ZmMGT10 Gene and Transformation of Arabidopsis thaliana[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(6): 1-7. doi: 10.7668/hbnxb.2017.06.001.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.06.001

http://www.hbnxb.net/EN/Y2017/V32/I6/1

References

[1] Cowan J A.Structural and catalytic chemistry of magnesium-dependent enzymes[J].Biometals:an International Journal on the Role of Metal Ions in Biology,Biochemistry,and Medicine,2002,15(3):225-235.
[2] Hörtensteiner S.Stay-green regulates chlorophyll and chlorophyll-binding protein degradation during senescence[J].Trends in Plant Science,2009,14(3):155-162.
[3] 靳晓琳,马翠兰,陈立松.植物缺镁研究进展[J].亚热带农业研究,2012,8(2):118-122.
[4] 盛翊佳,丁亚文,傅媛烨,等.植物应答镁营养胁迫机理的研究进展[J].植物学研究,2015,4(5):97-106.
[5] Bui D M,Gregan J,Jarosch E,et al.The bacterial magnesium transporter CorA can functionally substitute for its putative homologue Mrs2p in the yeast inner mitochondrial membrane[J].The Journal of Biological Chemistry,1999,274(29):20438-20443.
[6] Szegedy M A,Maguire M E.The CorA Mg²⁺ transport protein of Salmonella typhimurium:mutagenesis of conserved residues in the second membrane domain[J].Journal of Biological Chemistry,1999,274(44):36973-36979.
[7] Graschopf A,Stadler J A,Hoellerer M K,et al.The yeast plasma membrane protein Alr1 controls Mg²⁺ homeostasis and is subject to Mg²⁺-dependent control of its synthesis and degradation[J].Journal of Biological Chemistry,2001,276(19):16216-16222.
[8] Lee J M,Gardner R C.Residues of the yeast ALR1 protein that are critical for magnesium uptake[J].Current Genetics,2006,49(1):7-20.
[9] Pisat N P,Pandey A,Macdiarmid C W.MNR2 regulates intracellular magnesium storage in Saccharomyces cerevisiae[J].Genetics,2009,183(3):873-884.
[10] Li L,Tutone A F,Drummond R S,et al.A novel family of magnesium transport genes in Arabidopsis[J].The Plant Cell,2001,13(12):2761-2775.
[11] Saito T,Kobayashi N I,Tanoi K,et al.Expression and functional analysis of the CorA-MRS2-ALR-type magnesium transporter family in rice[J].Plant & Cell Physiology,2013,54(10):1673-1683.
[12] 张岗,翟清华,张大为,等.铁皮石斛镁离子转运蛋白基因的克隆及表达分析[J].中草药,2014,45(23):3443-3448.
[13] Li H,Du H,Huang K,et al.Identification,and functional and expression analyses of the CorA/MRS2/MGT-Type magnesium transporter family in maize[J].Plant & Cell Physiology,2016,57(6):1153-1168.
[14] 阳江华,秦云霞,方永军,等.巴西橡胶树镁离子转运蛋白基因 HbMGT10的克隆及表达分析[J].热带作物学报,2016,37(12):2353-2358.
[15] Deng W,Luo K,Li D,et al.Overexpression of an Arabidopsis magnesium transport gene, AtMGT1,in Nicotiana benthamiana confers Al tolerance[J].Journal of Experimental Botany,2006,57(15):4235-4243.
[16] Gebert M,Meschenmoser K,Svidová S,et al.A root-expressed magnesium transporter of the MRS2/MGT gene family in Arabidopsis thaliana allows for growth in low-Mg²⁺ environments[J].The Plant Cell,2009,21(12):4018-4030.
[17] Mao D,Chen J,Tian L,et al.Arabidopsis transporter MGT6 mediates magnesium uptake and is required for growth under magnesium limitation[J].The Plant Cell,2014,26(5):2234-2248.
[18] Oda K,Kamiya T,Shikanai Y,et al.The Arabidopsis Mg transporter,MRS2-4,is essential for Mg homeostasis under both low and high Mg conditions[J].Plant & Cell Physiology,2016,57(4):754-763.
[19] Clough S J,Bent A F.Floral Dip:a simplified method for agrobacterium-mediated transformation of Arabidopsis thaliana[J].Plant Journal,1998,16(6):735-743.
[20] Tocquin P,Corbesier L,Havelange A,et al.A novel high efficiency,low maintenance,hydroponic system for synchronous growth and flowering of Arabidopsis thaliana[J].BMC Plant Biology,2003,3:2.
[21] Grbić V,Bleecker A B.Ethylene regulates the timing of leaf senescence in Arabidopsis[J].Plant Journal,2003,8(8):595-602.
[22] Leggett J E,Gilbert W A.Magnesium uptake by soybeans[J].Plant Physiology,1969,44(8):1182-1186.
[23] Maas E V,Ogata G.Absorption of magnesium and chloride by excised corn root[J].Plant Physiology,1971,47(3):357-360.
[24] Joseph R A,Vanhai T.Kinetics of potassium and magnesium uptake by intact soybean roots[J].Physiologia Plantarum,1976,36(3):233-235.
[25] Rengel Z,Robinson D L.Competitive Al³⁺ inhibition of net Mg²⁺ uptake by intact Lolium multiflorum roots[J].Plant Physiology,1989,91:1407-1413.
[26] Tanoi K,Kobayashi N I,Saito T,et al.Application of Mg-28 to the kinetic study of Mg uptake by rice plants[J].Journal of Radioanalytical and Nuclear Chemistry,2013,296(2):749-751.
[27] Tanoi K,Kobayashi N I,Saito T,et al.Effects of magnesium deficiency on magnesium uptake activity of rice root,evaluated using (28) Mg as a tracer[J].Plant and Soil,2014,384(1/2):69-77.

Please choose a citation manager

Content to export