The Study of the Expression Pattern of Soybean sbp Gene and Activity Analysis of It's Promoter

doi:10.7668/hbnxb.2017.06.002

Abstract

Abstract: The expression pattern of soybean sbp gene and activity analysis of it's promoter was studied,which provided the theoretical foundation for further understanding the regulation of sbp gene expression and utilizing the promoter. The expression pattern of soybean sbp gene under different stresses and in soybean tissues were detected by qRT-PCR. The 5'-flanking upstream sequence of soybean sbp gene was isolated by PCR method and analyzed by some prediction softwares. The activity analysis of the promoter under drought stress and in different tissues were performed in transgenic tabacco. Soybean sbp gene was up-regulated by drought and were down-regulated by salt,cold and ABA. There was a little activity in roots,stems,leaves and flowers,but there was highest activity in seeds. The 5'-flanking upstream sequence of soybean sbp gene,named SP,was isolated,and the length was 942 bp. Sequence analysis revealed that this fragment contained a series of motifs related to seed-specific promoters,and stress-inducible promoters. The gus gene driven by SP promoter had high expression activity under drought stress or/and in seeds in transgenic tabacco through GUS histochemical analysis. SP promoter could have characteristics of drought-induced and seed-specific activity.

Key words: Soybean, sbp gene, Promoter, Cloning, Transgenic tobacco

CLC Number:

Q78
S565.03

ZHAO Yan, ZHAI Ying, LI Shanshan, YANG Xiaojie, LIU Lili. The Study of the Expression Pattern of Soybean sbp Gene and Activity Analysis of It's Promoter[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(6): 8-13. doi: 10.7668/hbnxb.2017.06.002.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.06.002

http://www.hbnxb.net/EN/Y2017/V32/I6/8

References

[1] Kühn C,Quick W P,Schulz A,et al. Companion cell-specific inhibition of the potato sucrose transporter SUT1[J]. Plant Cell and Environment,1996,19(10):1115-1123.
[2] 杨艳,王爽,黄丽燕,等.两个大豆GmSBP 基因的特征、亚细胞定位及对非生物胁迫的响应[J].生物工程学报,2014,30(11):1709-1719.
[3] 颜克亮,陈波,姚家玲,等.大豆种子形态建成期与成熟期正反抑制消减文库构建及差异表达基因分析[J].武汉大学学报:理学版,2008,54(2):202-208.
[4] Zhai Ying,Wang Ying,Li Yanjie,et al. Isolation and molecular characterization of GmERF7,a soybean ethylene-response factor that increases salt stress tolerance in tobacco[J]. Gene,2013,513(1):174-183.
[5] Zhao Y,Sha W,Wang Q Y,et al. Molecular cloning and activity analysis of a seed-specific FAD2-1B gene promoter from Glycine max[J]. Cellular and Molecular Biology,2015,61(4):85-89.
[6] Chen Yanhui,Han Yangyang,Zhang Meng,et al. Overexpression of the wheat expansin gene TaEXPA2 improved seed production and drought tolerance in transgenic tobacco plants[J]. PLoS One,2016,11(4):e0153494.
[7] Zhai Ying,Saho Shuli,Sha Wei,et al. Overexpression of soybean GmERF9 enhances the tolerance to drought and cold in the transgenic tobacco[J]. Plant Cell,Tissue and Organ Culture,2017,128(3):607-618.
[8] Li Xiaoshuang,Zhang Daoyuan,Li Haiyan,et al. EsDREB2B,a novel truncated DREB2-type transcription factor in the desert legume Eremosparton songoricum,enhances tolerance to multiple abiotic stresses in yeast and transgenic tobacco[J]. BMC Plant Biology,2014,14:44.
[9] Wei Zhigang,Qu Zanshuang,Zhang Lijie,et al. Overexpression of poplar xylem sucrose synthase in tobacco leads to a thickened cell wall and increased height[J]. PLoS One,2015,10(3):e0120669.
[10] Jefferson R A,Kavanagh T A,Bevan M W. GUS fusions-beta-glucuronidase as a sensitive and versatile gene fusion marker in higher-plants[J]. EMBO Journal,1987,6(13):3901-3907.
[11] Moreno-risueno M A,Gonzalez,Diazi,et al. FUSCA3 from barley unveils a common transcriptional regulation of seed-specific genes between cereals and Arabidopsis[J]. The Plant Journal:for Cell and Molecular Biology,2008,53(6):882-894.
[12] Chung K J,Hwang S K,Hahn B S,et al. Authentic seed-specific activity of the Perilla oleosin 19 gene promoter in transgenic Arabidopsis[J]. Plant Cell Reports,2008,27(1):29-37.
[13] Kim M J,Kim J K,Shin J S,et al. The SebHLH transcription factor mediates trans-activation of the SeFAD2 gene promoter through binding to E-and G-box elements[J]. Plant Molecular Biology,2007,64(4):453-466.
[14] Fauteux F,Strömvik M V. Seed storage protein gene promoters contain conserved DNA motifs in Brassicaceae,Fabaceae and Poaceae[J]. BMC Plant Biology,2009,9:126.
[15] Xie Chengjian,Zhang Beibei,Wang De,et al. Molecular cloning and characterization of an achene-seed-specific promoter from motherwort (Leonurus japonicus Houtt)[J]. Biotechnology Letters,2011,33(1):167-172.
[16] Hu Xinwen,Liu Sixin,Guo Jianchun,et al. Embryo and anther regulation of the mabinlin Ⅱ sweet protein gene in Capparis masaikai Lévl[J]. Functional & Integrative Genomics,2009,9(3):351-361.
[17] Gao Mingjun,Lydiate D J,Li Xiang,et al. Repression of seed maturation genes by a trihelix transcriptional repressor in Arabidopsis seedlings[J]. The Plant Cell,2009,21(1):54-71.
[18] Zhao Yan,Saho Shuli,Li Xiaowei,et al. Isolation and activity analysis of a seed-abundant soyAP1 gene promoter from soybean[J]. Plant Molecular Biology Reporter,2012,30(6):1400-1407.
[19] Zhou Qiyun,Tian Aiguo,Zou Hongfeng,et al. Soybean WRKY-type transcription factor genes, GmWRKY13,GmWRKY21,and GmWRKY54,confer differential tolerance to abiotic stresses in transgenic Arabidopsis-plants[J]. Plant Biotechnology Journal,2008,6(5):486-503.
[20] Nalbandi K,Kohnehrouz B B,Saeed K A,et al. Isolating barley(Hordeum vulgare L.)B1 hordein gene promoter and using sequencing analaysis for the identification of conservedregulatory elements by bioinformatic tools[J]. African Journal of Biotechnology,2012,11(29):7378-7387.

Please choose a citation manager

Content to export