Analysis on the Accumulation of Lignin in the Stem of Soybean

doi:10.7668/hbnxb.20192719

Abstract

Abstract:

To clarify the lignin accumulation ways in the stem of soybean variety,and further understand the relation between the lignin accumulation and lodging resistance of soybean plant,which could give the theory evidence for plant phenotype regulation and new soybean variety breeding under different planting densities,Charleston,a representative variety with determinate growth habit,was selected in current study and were planted with two densities of 20,35 plants/m².The lignin content in the elongation zone(EZ)and the secondary cell wall maturation zone(MZ)at the third internode of Charleston's stem were measured and the activities of key enzymes such as 4-Coumarate:coenzyme A ligase(4CL)and phenylalanine aminotransferase(PAL)and cinnamate dehydrogenase(CAD)involved in the lignin synthesis were determined at two different densities.The results showed that lignin content in the stem of Charleston under low density was higher than that under high density.The content of lignin in single internode(stem 3)of Charleston under both high and low density treatments was MZ>EZ.4CL,PAL and CAD activities in low density Charleston stem were higher than those in high density of Charleston and in accordance with gene expression levels.The key genes for lignin synthesized were distributed in soybean genome in different gene families.Moreover,different enzyme gene in one gene family showed different expression style in the same section.Same results could be observed in different organ for one gene.

Key words: Soybean, Stem, Lignin content, Key enzymes for lignin synthesis

LI Yanyan, ZHAO Caitong, QI Yangyang, LIU Ming, ZHANG Shurui, LIU Zhihua, LI Wenbin, JIANG Zhenfeng. Analysis on the Accumulation of Lignin in the Stem of Soybean[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 77-85. doi: 10.7668/hbnxb.20192719.

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Figures/Tables 8

Tab.1 Method procedure for 4CL activity

试剂名称 Reagent name	测定管/μL Measuring tube
样本 Sample	160
试剂一 Reagent 1	480
试剂二 Reagent 2	80
试剂三 Reagent 3	80

Fig.1 Resorcinol staining results of stem of Charleston under high density condition A,B and C are the staining results of the upper,middle and lower parts of the first stem segment respectively;D,E and F are the staining results of the upper,middle and lower parts of the second stem segment respectively;G,H and I are the staining results of the upper,middle and lower parts of the third stem segment,respectively.The same as Fig.2.

Fig.2 Resorcinol staining results of stem of Charleston under low density condition

Fig.3 Lignin content of stem of Charston under high and low densities A.Charleston different internode lignin content:CH.Charleston at high density,CL.Charleston at low density;B.Lignin content of EZ and MZ in stem 3:EZ.Cell elongation region,MZ.Mature area of secondary cell wall,HⅢ.Stem 3 of Charleston under high density treatment,LⅢ.Stem 3 of Charleston under low density treatment.Different lowercase letters indicate significant difference (Duncan's multiple-range test,P<0.05). The same as Fig.4-6.

Tab.2 Metabolite content of phenylpropane synthesis pathway and related genes under high density conditions

代谢物 Metabolites	EZ/AS	MZ/EZ	基因 Gene
肉桂酸 Cinnamic acid	-2.1		PAL	Glyma.13g145000	-3.77
				Glyma.20g180800	-2.30
				Glyma.03g181600	3.22
对香豆醇 P-coumarylalcohol	2.4		4CL	Glyma.13g372000	-2.18
松柏醛 Coniferylaldehyde	1.7	2.8	CCR	Glyma.13g369800	-0.29
				Glyma.08g218100	-1.10
松柏醇 Coniferyl alcohol	1.7	4.0	CAD	Glyma.20g128600	-1.80

Fig.4 PAL activity and PAL genes in EZ and MZ of stem of Charleston in different density A.PAL gene expression levels of EZ and MZ in stem of Charleston section 3 under high density treatment;B.PAL activity in stem of Charleston treated with high and low density:Ⅱ.The second internode,Ⅲ1.The third internode EZ,Ⅲ2.The third internode MZ,Ⅳ.The fourth internode.The same as Fig.5-6.

Fig.5 4CL activity and 4CL genes in EZ and MZ of stem of Charleston in different density A.4CL gene expression levels of EZ and MZ in stem of Charleston section 3 under high density treatment; B.4CL activity in stem of Charleston treated with high and low density.

Fig.6 CAD activity and CAD genes in EZ and MZ of stem of Charleston in different density A.CAD gene expression levels of EZ and MZ in stem of Charleston section 3 under high density treatment; B.CAD activity in stem of Charleston treated with high and low density.

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