Abstract: Aiming at the problem of the narrow genetic basis of the EPSPS gene of glyphosate resistant transgenic soybean,using ms1 recurrent population to broaden soybean genetic basis was studied.There are three populations.The first is the basic population that has rich diversity,the second is C0 population that was formed based the basic population and transgenic lines Ji K32,Ji K331,Ji K69,Ji K964.The third is C1 population that derived from C0. The quality,yield and other agronomic traits of the three populations were analyzed. The results showed that C1 population protein changed from 35.66% to 48.02%;fatty changed from 16.34% to 22.92%;plant height varied from 46.00 cm to 158.00 cm;branch number varied from 0 to 8,100 seeds weight changed from 10.30 g to 25.30 g;plant weight changes range from 1.60 to 59.60 g. The proportion of male sterile plants in C1 population was 19.20%,and the proportion of glyphosate resistant plants was 70.54%.Using soybean ms1 gene to construct a EPSPS gene containing genetically modified soybean recurrent population,the population has rich genetic diversity and is suitable for transgenic soybean breeding material selection.

Key words: Soybean, Recurrent selection population, ms1 gene, Transgenic, EPSPS, Glyphosate

CLC Number: 

• Q78

• S656.03