Effects of Nitrogen Nutrition and Rhizobium Inoculation on Nodulation and Nitrogen Fixation and Growth of Soybean

doi:10.7668/hbnxb.20192484

Abstract

Abstract:

To study the effect of soybean rhizobia inoculation under different nitrogen levels on soybean growth and nodulation and nitrogen fixation,soybean Williams 82 was used as the test material to be planted by vermiculite method,and nitrate treatments with three concentrations of no nitrogen,low nitrogen and high nitrogen were set up.Inoculate USDA110 soybean rhizobia to study the effect of different nitrogen levels on soybean nodulation and nitrogen content.The results showed that different levels of nitrogen application had different effects on the number of nodules,nodule dry weight and root dry weight.The application of high-nitrogen nutrient solution could inhibit soybean nodulation,while no nitrogen treatment promoted soybean nodulation.At the same time,the root dry weight showed the opposite trend,the root dry mass of the no nitrogen treatment was the smallest,and the root dry mass of the high-nitrogen treatment was the largest.Under different nitrogen levels,the nitrogen content of different organs of soybean showed different changing trends between inoculation and non-inoculation.Under different nitrogen levels,the nitrogen content under high nitrogen treatment was higher than low nitrogen and no nitrogen treatment,and the nitrogen content of different organs showed the phenomenon of leaf>root>stem overall.Under different nitrogen levels,the chlorophyll values(SPAD)of different leaf positions showed that there was no significant difference between inoculation and non-inoculation.The measurement results of leaf morphological indicators showed that the leaf area,leaf circumference,leaf length and leaf width of high nitrogen treatment were significantly higher than low nitrogen and no nitrogen treatments.Leaf area and leaf circumference after nitrogen-free inoculation were significantly greater than those without nitrogen-free inoculation,indicating that inoculation not only promoted root growth,but also stimulated leaf enlargement.It can be seen that rhizobium inoculation had different changes for nodulation,nitrogen fixation and growth and development of soybean seedlings under different nitrogen levels.Appropriate increase in nitrogen nutrition combined with the use of rhizobia was an effective way to promote the growth and development of soybean and the utilization and absorption of nitrogen.

Key words: Soybean, Nitrogen level, Nodulation and nitrogen fixation, Nitrogen Accumulation, Growth

WANG Xiaoli, WANG Min, YUE Aiqin, GUO Shujin, WANG Peng, WANG Lixiang, YANG Tingting, ZHANG Haisheng, ZHANG Yongpo, GAO Chunyan, ZHANG Wuxia, NIU Jingping, DU Weijun, ZHAO Jinzhong. Effects of Nitrogen Nutrition and Rhizobium Inoculation on Nodulation and Nitrogen Fixation and Growth of Soybean[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 95-102. doi: 10.7668/hbnxb.20192484.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192484

http://www.hbnxb.net/EN/Y2022/V37/I1/95

Figures/Tables 6

References 29

[1]	贾志荣,张美俊,杨武德.施氮对套作玉米氮素吸收利用的影响[J].山西农业科学,2017,45(12):1960-1964,2031.doi:10.3969/j.issn.1002-2481.2017.12.16.
	Jia Z R,Zhang M J,Yang W D.Effects of nitrogen fertilizer on nitrogen uptake and utilization of intercropped maize[J].Journal of Shanxi Agricultural Sciences,2017,45(12):1960-1964,2031.
[2]	Chun L,Mi G H,Li J S,Chen F J,Zhang F S.Genetic analysis of maize root characteristics in response to low nitrogen stress[J].Plant and Soil,2005,276(1/2):369-382.doi:10.1007/s11104-005-5876-2.
[3]	李强,罗延宏,余东海,孔凡磊,杨世民,袁继超.低氮胁迫对耐低氮玉米品种苗期光合及叶绿素荧光特性的影响[J].植物营养与肥料学报,2015,21(5):1132-1141.doi:10.11674/zwyf.2015.0505.
	Li Q,Luo Y H,Yu D H,Kong F L,Yang S M,Yuan J C.Effects of low nitrogen stress on photosynthetic characteristics and chlorophyll fluorescence parameters of maize cultivars tolerant to low nitrogen stress at the seedling stage[J].Journal of Plant Nutrition and Fertilizers,2015,21(5):1132-1141.
[4]	孙年喜,宗学凤,王三根.不同供氮水平对玉米光合特性的影响[J].西南农业大学学报(自然科学版),2005,27(3):389-392,396.doi:10.3969/j.issn.1673-9868.2005.03.027.
	Sun N X,Zong X F,Wang S G.Effects of nitrogen supply on photosynthetic traits of maize[J].Journal of Southwest Agricultural University(Natural Science),2005,27(3):389-392,396.
[5]	Liu Y,Li M X,Xu J S,Liu X Y,Wang S Y,Shi L X.Physiological and metabolomics analyses of young and old leaves from wild and cultivated soybean seedlings under low-nitrogen conditions[J].BMC Plant Biology,2019,19(1):389.doi:10.1186/s12870-019-2005-6.
[6]	Ren B,Wang X T,Duan J B,Ma J X.Rhizobial tRNA-derived small RNAs are signal molecules regulating plant nodulation[J].Science,2019,365(6456):919-922.doi:10.1126/science.aav8907.
[7]	梁福琴,关大伟,党蓓蕾,樊晨,张强,王晓霞,吴菊梅.根瘤菌和氮素对大豆植株特性及产量的影响[J].黑龙江农业科学,2017(8):28-31.doi:10.1194/j.issn1002-2767.2017.08.0028.
	Liang F Q,Guan D W,Dang B L,Fan C,Zhang Q,Wang X X,Wu J M.Effect of rhizobium and nitrogen on plant characteristics and yield of soybean[J].Heilongjiang Agricultural Sciences,2017(8):28-31.
[8]	刘琪,乔月静,高志强.夏闲期不同耕作措施对旱地小麦根际土壤微生物的影响[J].山西农业科学,2019,47(1):65-68.doi:10.3969/j.issn.1002-2481.2019.01.16.
	Liu Q,Qiao Y J,Gao Z Q.Effects of different tillage practices on rhizosphere soil microorganisms in dryland wheat during summer leisure period[J].Shanxi Agricultural Sciences,2019,47(1):65-68.
[9]	Agtuca B J,Stopka S A,Evans S,Samarah L,Liu Y,Xu D,Stacey M G,Koppenaal D W,Paša-Tolic' L,Anderton C R,Vertes A,Stacey G.Metabolomic profiling of wild-type and mutant soybean root nodules using laser-ablation electrospray ionization mass spectrometry reveals altered metabolism[J].The Plant Journal,2020,103(5):1937-1958.doi:10.1111/tpj.14815.
[10]	Gan Y B,Stulen I,van Keulen H,Kuiper P J C.Low concentrations of nitrate and ammonium stimulate nodulation and N₂ fixation while inhibiting specific nodulation(nodule DW g^-1 root dry weight)and specific N₂ fixation(N₂ fixed g^-1 root dry weight)in soybean[J].Plant and Soil,2004,258(1):281-292.doi:10.1023/B:PLSO.0000016558.32575.17.
[11]	王浩,刘伟,姜妍,王绍东,李宏伟,李远明.不同氮素水平下接种根瘤菌对大豆生长的影响[J].大豆科技,2012(1):14-17.doi:10.3969/j.issn.1674-3547.2012.01.007.
	Wang H,Liu W,Jiang Y,Wang S D,Li H W,Li Y M.Effect of rhizobium inoculation and nitrogen level on growth of soybean[J].Soybean Science and Technology,2012(1):14-17.
[12]	伍惠,钟喆栋,樊伟,彭亚齐,杜思,陈大松,王学路,李友国.8株优良大豆根瘤菌与不同地区27个大豆主栽品种的匹配性研究[J].大豆科学,2017,36(3):405-418.doi:10.11861/j.issn.1000-9481/2017.
	Wu H,Zhong Z D,Fan W,Peng Y Q,Du S,Chen D S,Wang X L,Li Y G.Symbiotic compatibility among eight elite soybean rhizobia strains and twenty-seven soybean cultivars from different planting regions[J].Soybean Science,2017,36(3):405-418.
[13]	Yuan S L,Li R,Chen S L,Chen H F,Zhang C J,Chen L M,Hao Q N,Shan Z H,Yang Z L,Qiu D Z,Zhang X J,Zhou X N.RNA-seq analysis of differential gene expression responding to different Rhizobium strains in soybean(Glycine max)roots[J].Frontiers in Plant Science,2016,7:721.doi:10.3389/fpls.2016.00721.
[14]	Wang R C,Okamoto M,Xing X J,Crawford N M.Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1 000 rapidly responding genes and new linkages to glucose,trehalose-6-phosphate,iron,and sulfate metabolism[J].Plant Physiology,2003,132(2):556-567.doi:10.1104/pp.103.021253.
[15]	Walch-Liu P,Forde B G.Nitrate signalling mediated by the NRT1.1 nitrate transporter antagonises L-glutamate-induced changes in root architecture[J].The Plant Journal,2008,54(5):820-828.doi:10.1111/j.1365-313x.2008.03443.x.
[16]	郑永美,王春晓,刘岐茂,吴正锋,王才斌,孙秀山,郑亚萍.氮肥对花生根系生长和结瘤能力的调控效应[J].核农学报,2017,31(12):2418-2425.doi:10.11869/j.issn.100-8551.2017.12.2418.
	Zheng Y M,Wang C X,Liu Q M,Wu Z F,Wang C B,Sun X S,Zheng Y P.Effect of nitrogen fertilizer regulation on root growth and nodulating ability of peanut[J].Journal of Nuclear Agricultural Sciences,2017,31(12):2418-2425.
[17]	Castro Marín I,Loef I,Bartetzko L,Searle I,Coupland G,Stitt M,Osuna D.Nitrate regulates floral induction in Arabidopsis,acting independently of light,gibberellin and autonomous pathways[J].Planta,2011,233(3):539-552.doi:10.1007/s00425-010-1316-5.
[18]	康佳惠,梁秀芝,郑敏娜,韩志顺,陈燕妮.不同外源氮素形态对紫花苜蓿根系的影响[J].山西农业科学,2021,49(4):467-471.doi:10.3969/j.issn.1002-2481.2021.04.14.
	Kang J H,Liang X Z,Zheng M N,Han Z S,Chen Y N.Effects of exogenous nitrogen forms on the root of alfalfa[J].Journal of Shanxi Agricultural Sciences,2021,49(4):467-471.
[19]	江木兰,张学江,徐巧珍,胡小加,张银波.大豆—根瘤菌的固氮作用[J].中国油料作物学报,2003,25(1):50-53,58.doi:10.3321/j.issn:1007-9084.2003.01.013.
	Jiang M L,Zhang X J,Xu Q Z,Hu X J,Zhang Y B.Nodulation and nitrogen-fixation in soybean-rhizobium[J].Chinese Journal of Oil Crop Sciences,2003,25(1):50-53,58.
[20]	Liu Y H,Wang E T,Jiao Y S,Tian C F,Wang L,Wang Z J,Guan J J,Singh R P,Chen W X,Chen W F.Symbiotic characteristics of Bradyrhizobium diazoefficiens USDA 110 mutants associated with shrubby sophora(Sophora flavescens)and soybean(Glycine max)[J].Microbiological Research,2018,214:19-27.doi:10.1016/j.micres.2018.05.012.
[21]	Forde B G.Nitrogen signalling pathways shaping root system architecture:An update[J].Current Opinion in Plant Biology,2014,21:30-36.doi:10.1016/j.pbi.2014.06.004.
[22]	Meng L,Dong J X,Wang S S,Song K,Ling A F,Yang J G,Xiao Z X,Li W,Song W J,Liang H B.Differential responses of root growth to nutrition with different ammonium/nitrate ratios involve auxin distribution in two tobacco cultivars[J].Journal of Integrative Agriculture,2019,18(12):2703-2715.doi:10.1016/S2095-3119(19)62595-5.
[23]	Liu Y,Wu L,Baddeley J A,Watson C A.Models of biological nitrogen fixation of legumes[J].Springer Netherlands,2011,2:883-905.doi:10.1007/978-94-007-0394-0_39.
[24]	Sun J L,Ye M,Peng S B,Li Y.Nitrogen can improve the rapid response of photosynthesis to changing irradiance in rice(Oryza sativa L.)plants[J].Scientific Reports,2016,6:31305.doi:10.1038/srep31305.
[25]	Li Y S, Yu Z H, Liu X B, Mathesius U, Wang G H, Tang C X, Wu J J, Liu J D, Zhang S Q, Jin J.Elevated CO₂ increases nitrogen fixation at the reproductive phase contributing to various yield responses of soybean cultivars[J].Frontiers in Plant Science,2017,8:1546.doi:10.3389/fpls.2017.01546.
[26]	王树起,韩晓增,乔云发,严君,李晓慧.施氮对大豆根瘤生长和结瘤固氮的影响[J].华北农学报,2009,24(2):176-179.doi:10.7668/hbnxb.2009.02.036.
	Wang S Q,Han X Z,Qiao Y F,Yan J,Li X H.Nodule growth, nodulation and nitrogen fixation in soybean(Glycine max L.)as affected by nitrogen application[J].Acta Agriculturae Boreali-Sinica, 2009,24(2):176-179.
[27]	Netto A T,Campostrini E,de Oliveira J G,Bressan-Smith R E.Photosynthetic pigments,nitrogen,chlorophyll a fluorescence and SPAD-502 readings in coffee leaves[J].Scientia Horticulturae,2005,104(2):199-209.doi:10.1016/j.scienta.2004.08.013.
[28]	Jia M,Colombo R,Rossini M,Celesti M,Zhu J,Cogliati S,Cheng T,Tian Y C,Zhu Y,Cao W X,Yao X.Estimation of leaf nitrogen content and photosynthetic nitrogen use efficiency in wheat using sun-induced chlorophyll fluorescence at the leaf and canopy scales[J].European Journal of Agronomy,2021,122:126192.doi:10.1016/j.eja.2020.126192.
[29]	Lawlor D W.Carbon and nitrogen assimilation in relation to yield:Mechanisms are the key to understanding production systems[J].Journal of Experimental Botany,2002,53(370):773-787.doi:10.1093/jexbot/53.370.773.

指标 Indexes	对照 CK			接菌 Inoculation
指标 Indexes	无氮 NN	低氮 LN	高氮 HN	无氮 NN	低氮 LN	高氮 HN
根瘤数目/个	0	0	0	193.670±2.190a	84.000±10.650b	30.000±5.030c
Nodule number
根瘤干质量/g	0	0	0	0.072±0.004a	0.035±0.004b	0.004±0.001c
Nodule dry weight
根干质量/g	0.257±0.001b	0.297±0.010b	0.367±0.013a	0.275±0.007b	0.287±0.008b	0.351±0.002a
Root dry weight

指标 Indexes	对照 CK			接菌 Inoculation
指标 Indexes	无氮 NN	低氮 LN	高氮 HN	无氮 NN	低氮 LN	高氮 HN
根瘤数目/个	0	0	0	193.670±2.190a	84.000±10.650b	30.000±5.030c
Nodule number
根瘤干质量/g	0	0	0	0.072±0.004a	0.035±0.004b	0.004±0.001c
Nodule dry weight
根干质量/g	0.257±0.001b	0.297±0.010b	0.367±0.013a	0.275±0.007b	0.287±0.008b	0.351±0.002a
Root dry weight

处理 Treatment		叶位 Leaf position	叶面积/mm² Leaf area	叶周长/mm Leaf perimeter	叶长/mm Leaf length	叶宽/mm Leaf width
对照	无氮	第1对真叶	960.82±86.82Ba	119.27±4.96Ba	39.19±1.6Bab	32.48±1.57Ba
CK	NN	第1叶	1 056.32±103.51Ba	121.14±5.75Ba	42.40±2.05Ba	31.75±1.59Ba
		第2叶	560.87±107.02Bb	87.68±8.85Bb	31.65±3.48Bb	22.54±2.13Bb
	低氮	第1对真叶	1 574.55±128.65Aa	153.31±3.82Aa	50.70±1.64Aa	41.18±1.88Aa
	LN	第1叶	1 741.81±124.89Aa	156.37±5.70Aa	54.49±2.09Aa	41.64±2.21Aa
		第2叶	1 566.09±142.64Aa	148.20±6.37Aa	52.63±2.44Aa	39.14±2.05Aa
	高氮	第1对真叶	1 737.36±201.81Aa	159.59±8.62Aa	54.07±2.35Aa	42.93±2.20Aa
	HN	第1叶	1 966.78±69.79Aa	167.09±2.98Aa	59.62±0.92Aa	42.99±1.03Aa
		第2叶	1 639.54±130.01Aa	151.26±6.08Aa	52.47±2.65Aa	40.77±1.46Aa
接菌	无氮	第1对真叶	1 448.27±34.53ABb	144.80±1.85ABb	48.36±0.61Bb	39.50±0.24ABab
Inoculation	NN	第1叶	1 849.04±58.11Aa	161.34±2.67Aa	57.48±0.84Aa	42.24±1.12Aa
		第2叶	1 449.32±95.17Ab	144.86±5.20Ab	52.80±2.31Aab	36.47±1.29Ab
	低氮	第1对真叶	1 209.60±99.79Bb	132.18±5.44Ba	43.05±1.46Cb	37.50±2.27Bb
	LN	第1叶	1 649.18±82.08Aa	151.92±3.74Aa	52.24±1.29Ba	41.09±1.33Aa
		第2叶	1 411.30±141.86Aab	139.69±6.86Ab	48.71±2.80Aab	37.66±1.73Ab
	高氮	第1对真叶	1 791.58±125.51Aa	161.99±5.26Aa	53.05±1.23Aa	45.24±2.49Aa
	HN	第1叶	1 767.41±63.83Aa	158.85±2.92Aa	56.20±1.16ABa	41.89±0.64Aa
		第2叶	1 738.14±146.06Aa	156.75±7.38Aa	54.57±3.16Aa	41.64±1.92Aa

处理 Treatment		叶位 Leaf position	叶面积/mm² Leaf area	叶周长/mm Leaf perimeter	叶长/mm Leaf length	叶宽/mm Leaf width
对照	无氮	第1对真叶	960.82±86.82Ba	119.27±4.96Ba	39.19±1.6Bab	32.48±1.57Ba
CK	NN	第1叶	1 056.32±103.51Ba	121.14±5.75Ba	42.40±2.05Ba	31.75±1.59Ba
		第2叶	560.87±107.02Bb	87.68±8.85Bb	31.65±3.48Bb	22.54±2.13Bb
	低氮	第1对真叶	1 574.55±128.65Aa	153.31±3.82Aa	50.70±1.64Aa	41.18±1.88Aa
	LN	第1叶	1 741.81±124.89Aa	156.37±5.70Aa	54.49±2.09Aa	41.64±2.21Aa
		第2叶	1 566.09±142.64Aa	148.20±6.37Aa	52.63±2.44Aa	39.14±2.05Aa
	高氮	第1对真叶	1 737.36±201.81Aa	159.59±8.62Aa	54.07±2.35Aa	42.93±2.20Aa
	HN	第1叶	1 966.78±69.79Aa	167.09±2.98Aa	59.62±0.92Aa	42.99±1.03Aa
		第2叶	1 639.54±130.01Aa	151.26±6.08Aa	52.47±2.65Aa	40.77±1.46Aa
接菌	无氮	第1对真叶	1 448.27±34.53ABb	144.80±1.85ABb	48.36±0.61Bb	39.50±0.24ABab
Inoculation	NN	第1叶	1 849.04±58.11Aa	161.34±2.67Aa	57.48±0.84Aa	42.24±1.12Aa
		第2叶	1 449.32±95.17Ab	144.86±5.20Ab	52.80±2.31Aab	36.47±1.29Ab
	低氮	第1对真叶	1 209.60±99.79Bb	132.18±5.44Ba	43.05±1.46Cb	37.50±2.27Bb
	LN	第1叶	1 649.18±82.08Aa	151.92±3.74Aa	52.24±1.29Ba	41.09±1.33Aa
		第2叶	1 411.30±141.86Aab	139.69±6.86Ab	48.71±2.80Aab	37.66±1.73Ab
	高氮	第1对真叶	1 791.58±125.51Aa	161.99±5.26Aa	53.05±1.23Aa	45.24±2.49Aa
	HN	第1叶	1 767.41±63.83Aa	158.85±2.92Aa	56.20±1.16ABa	41.89±0.64Aa
		第2叶	1 738.14±146.06Aa	156.75±7.38Aa	54.57±3.16Aa	41.64±1.92Aa

Please choose a citation manager

Content to export