Regulatory Effects of Two Grain Filling Regulators on Inferior Spikelets Filling of Large Panicle Rice

doi:10.7668/hbnxb.20192499

Abstract

Abstract:

In order to explore the effects and mechanism of grain filling regulators on inferior spikelets filling of large panicle rice varieties,large panicle rice variety Jiaoyuanyou 216 was selected as material and an open field experiment was conducted by spraying exogenous grain filling regulators(Helifeng and Xinmeizhouxing)at heading stage.Meanwhile,endogenous hormone contents,expressions of filling-related miRNAs and their targets,and genes encoding filling-related proteins and sucrose-starch metabolism key enzymes were measured.The results should that the two grain filling regulators of Helifeng and Xinmeizhouxing significantly increased the yield of Jiaoyuanyou 216,the 1000-grain weight,filling rate and initial grain filling potential of inferior spikelets.Specifically,compared with the control(spraying water),the 1000-grain weight of inferior spikelets increased by 16.07% and 15.89%,respectively.Content of IAA in inferior spikelets increased significantly at 9,15,21 d after flowering,and the content of Z+ZR increased significantly at 9,21 d after flowering.Under Helifeng treatment,expressions of miR167a-c,miR167d-j and miR1432 were significantly down-regulated at 6 d after flowering,OsGF14b and OsGF14f were significantly down-regulated at 12 after flowering. What's more,expression of OsGLP3,a gene encoding germin-like protein was significantly up-regulated at 6 d after flowering,and expressions of sucrose-starch metabolism key enzyme related genes were significantly up-regulated at 6,12 d after flowering.However,under Xinmeizhouxing treatment,expressions of miR167a-c,miR167d-j,miR1432 were significantly down-regulated at 6 d after flowering,and expressions of OsGF14b and OsGF14f were significantly down-regulated at 12 d after flowering.More importantly,expressions of OsGLP3 and sucrose-starch metabolism key enzyme related genes were significantly up-regulated at 6,12 d after flowering.Correlation analysis results showed that the filling rate of inferior spikelets was significantly negatively correlated with the expression of miR1432 and OsGF14f,while was significantly positively correlated with the content of IAA.Therefore,Helifeng and Xinmeizhouxing may regulate the expressions of filling-related miRNAs and their targets,as well as the genes of filling-related proteins and sucrose-starch metabolism key enzymes in the pre-and mid-filling period and increase the content of IAA and Z+ZR to promote inferior spikelets filling.

Key words: Rice, Inferior spikelets, Grain filling, Regulators, miRNA, Sucrose-starch metabolism key enzyme related genes

JING Lili, PENG Ting, ZHAO Yafan, ZHAO Shuaibing, WANG Tongtong, LI Yuan, CHENG Yuan, DU Yanxiu, ZHANG Jing, SUN Hongzheng, ZHAO Quanzhi. Regulatory Effects of Two Grain Filling Regulators on Inferior Spikelets Filling of Large Panicle Rice[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 86-94. doi: 10.7668/hbnxb.20192499.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192499

http://www.hbnxb.net/EN/Y2022/V37/I1/86

Figures/Tables 9

References 28

[1]	蒋丹,文欢,潘珊珊,兰诗琪,南文斌,梁永书,张汉马,秦小健.一份水稻大粒材料的粒型及生理特性分析[J].华北农学报,2020,35(S1):126-132.doi:10.7668/hbnxb.20191502.
	Jiang D,Wen H,Pan S S,Lan S Q,Nan W B,Liang Y S,Zhang H M,Qin X J.Analysis of grain type and physiological characteristics for a large grain rice[J].Acta Agriculturae Boreali-Sinica,2020,35(S1):126-132.
[2]	Zhang H,Zhang J S,Yan J,Gou F,Mao Y F,Tang G L,Botella J R,Zhu J K.Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits[J].Proceedings of the National Academy of Sciences of the United States of America,2017,114(20):5277-5282.doi:10.1073/pnas.1703752114.
[3]	Peng T,Qiao M M,Liu H P,Teotia S,Zhang Z H,Zhao Y F,et al.A resource for inactivation of MicroRNAs using short tandem target mimic technology in model and crop plants[J].Molecular Plant,2018,11(11):1400-1417.doi:10.1016/j.molp.2018.09.003.
[4]	Zhao Y F,Peng T,Sun H Z,Teotia S,Wen H L,Du Y X,Zhang J,Li J Z,Tang G L,Xue H W,Zhao Q Z.miR1432-OsACOT(Acyl-CoA thioesterase)module determines grain yield via enhancing grain filling rate in rice[J].Plant Biotechnology Journal,2019,17(4):712-723.doi:10.1111/pbi.13009.
[5]	Zhang Z X,Zhao H,Huang F L,Long J F,Song G,Lin W X.The 14-3-3 protein GF14f negatively affects grain filling of inferior spikelets of rice(Oryza sativa L.)[J].The Plant Journal,2019,99(2):344-358.doi:10.1111/tpj.14329.
[6]	Zhang Z X,Tang J,Du T W,Zhao H,Li Z,Li Z,Lin W X.Mechanism of developmental stagnancy of rice inferior spikelets at early grain-filling stage as revealed by proteomic analysis[J].Plant Molecular Biology Reporter,2015,33(6):1844-1863.doi:10.1007/s11105-015-0880-z.
[7]	V P,Tyagi A.Correlation between expression and activity of ADP glucose pyrophosphorylase and starch synthase and their role in starch accumulation during grain filling under drought stress in rice[J].Plant Physiology and Biochemistry,2020,157:239-243.doi:10.1016/j.plaphy.2020.10.018.
[8]	Han H X,Yang C T,Zhu J H,Zhang L X,Bai Y M,Li E P,Gilbert R G.Competition between granule bound starch synthase and starch branching enzyme in starch biosynthesis[J].Rice,2019,12(1):96.doi:10.1186/s12284-019-0353-3.
[9]	Berahim Z,Dorairaj D,Saud H M,Ismail M R.Regulation of sucrose synthase and its association with grain filling in spermine-treated rice plant under water deficit[J].Journal of Plant Interactions,2019,14(1):464-473.doi:10.1080/17429145.2019.1657189.
[10]	徐云姬,许阳东,李银银,钱希旸,王志琴,杨建昌.干湿交替灌溉对水稻花后同化物转运和籽粒灌浆的影响[J].作物学报,2018,44(4):554-568.doi:10.3724/SP.J.1006.2018.00554.
	Xu Y J,Xu Y D,Li Y Y,Qian X Y,Wang Z Q,Yang J C.Effect of alternate wetting and drying irrigation on post-anthesis remobilization of assimilates and grain filling of rice[J].Acta Agronomica Sinica,2018,44(4):554-568.
[11]	褚光,徐冉,陈松,徐春梅,王丹英,章秀福.干湿交替灌溉对籼粳杂交稻产量与水分利用效率的影响及其生理基础[J].中国农业科学,2021,54(7):1499-1511.doi:10.3864/j.issn.0578-1752.2021.07.014.
	Chu G,Xu R,Chen S,Xu C M,Wang D Y,Zhang X F.Effects of alternate wetting and soil drying on the grain yield and water use efficiency of indica-japonica hybrid rice and its physiological bases[J].Scientia Agricultura Sinica,2021,54(7):1499-1511.
[12]	张志兴,陈花,敏秀梅,许海龙,宋果,林文雄.多肽配体R18促进水稻弱势籽粒灌浆的初步研究[J].作物学报,2021,47(7):1332-1341.doi:10.3724/SP.J.1006.2021.02049.
	Zhang Z X,Chen H,Min X M,Xu H L,Song G,Lin W X.Preliminary study of the peptide aptamer R18 promotes grain filling of rice inferior spikelets[J].Acta Agronomica Sinica,2021,47(7):1332-1341.
[13]	Zhang H,Tan G L,Yang L N,Yang J C,Zhang J H,Zhao B H.Hormones in the grains and roots in relation to post-anthesis development of inferior and superior spikelets in japonica/indica hybrid rice[J]. Plant Physiology and Biochemistry,2009,47(3):195-204.doi:10.1016/j.plaphy.2008.11.012.
[14]	Zhao B H,Liu K,Zhang H X,Zhu Q S,Yang J C.Causes of poor grain plumpness of two-line hybrids and their relationships to the contents of hormones in the rice grain[J].Agricultural Sciences in China,2007,6(8):930-940.doi:10.1016/S1671-2927(07)60131-X.
[15]	唐瑭,谢红,吕冰,梁建生.植物激素对杂交稻籽粒灌浆及蔗糖合酶活性的影响[J].中国水稻科学,2011,25(2):182-188.doi:10.3969/j.issn.1001-7216.2011.02.010.
	Tang T,Xie H,Lü B,Liang J S.Effects of plant hormones in regulating sucrose synthase activity and grain filling of rice[J].Chinese Journal of Rice Science,2011,25(2):182-188.
[16]	Zhang W Y,Cao Z Q,Zhou Q,Chen J,Xu G W,Gu J F,Liu L J,Wang Z Q,Yang J C,Zhang H.Grain filling characteristics and their relations with endogenous hormones in large-and small-grain mutants of rice[J].PLoS One,2016,11(10):e0165321.doi:10.1371/journal.pone.0165321.
[17]	陈海生.外源生长素对亚种间杂交稻两优培九籽粒生理活性和结实率的作用[J].安徽农业科学,2012,40(1):80,139.doi:10.13989/j.cnki.0517-6611.2012.01.189.
	Chen H S.Effects of exogenous IAA on physiological activities and seed setting rate in grain of intersubspecific hybrid rice Liangyoupeijiu[J].Journal of Anhui Agricultural Sciences,2012,40(1):80,139.
[18]	聂波. 植物生长调节剂在水稻生产上的应用分析[J].当代化工研究,2020(17):159-160.doi:10.3969/j.issn.1672-8114.2020.17.076.
	Nie B.Application analysis of plant growth regulator in rice production[J].Modern Chemical Research,2020(17):159-160.
[19]	顾俊荣,董明辉,杨代凤,陈培峰,王文青,乔中英.一种新型水稻生长调节剂对超级稻籽粒结实与品质特性的影响[J].江西农业学报,2017,29(12):8-13.doi:10.19386/j.cnki.jxnyxb.2017.12.02.
	Gu J R,Dong M H,Yang D F,Chen P F,Wang W Q,Qiao Z Y.Effects of a kind of new-type rice growth regulator on grain setting traits and quality of super rice[J].Acta Agriculturae Jiangxi,2017,29(12):8-13.
[20]	秦猛,刘丽华,郑桂萍,康楷,李红宇,吕艳东,陈志波,孙维声.不同叶面肥及施用时期对水稻穗部性状及产量、品质的影响[J].河南农业科学,2020,49(9):20-26.doi:10.15933/j.cnki.1004-3268.2020.09.003.
	Qin M,Liu L H,Zheng G P,Kang K,Li H Y,Lü Y D,Chen Z B,Sun W S.Effects of different foliar fertilizers and application periods on panicle traits,yield and quality of rice[J].Journal of Henan Agricultural Sciences,2020,49(9):20-26.
[21]	彭廷,万建伟,王琳琳,杜彦修,张静,李俊周,孙红正,赵全志.复配化学调节剂对水稻子粒灌浆充实和蔗糖-淀粉代谢关键基因表达的影响[J].河南农业大学学报,2015,49(5):602-607,615.doi:10.16445/j.cnki.1000-2340.2015.05.005.
	Peng T,Wan J W,Wang L L,Du Y X,Zhang J,Li J Z,Sun H Z,Zhao Q Z.Effect of chemical regulation compounds on rice grain filling,and sucrose-starch metabolism key enzyme related genes expression of rice[J].Journal of Henan Agricultural University,2015,49(5):602-607,615.
[22]	Peng T,Lü Q,Zhao Y F,Sun H Z,Han Y C,Du Y X,Zhang J,Li J Z,Wang L L,Zhao Q Z.Superior grains determined by grain weight are not fully correlated with the flowering order in rice[J].Journal of Integrative Agriculture,2015,14(5):847-855.doi:10.1016/S2095-3119(14)60858-3.
[23]	朱庆森,曹显祖,骆亦其.水稻籽粒灌浆的生长分析[J].作物学报,1988,14(3):182-193.doi:10.3321/j.issn:0496-3490.1988.03.002.
	Zhu Q S,Cao X Z,Luo Y Q.Growth analysis on the process of grain filling in rice[J].Acta Agronomica Sinica,1988,14(3):182-193.
[24]	Rogers K,Chen X M.Biogenesis,turnover,and mode of action of plant MicroRNAs[J].The Plant Cell,2013,25(7):2383-2399.doi:10.1105/tpc.113.113159.
[25]	You C C,Zhu H L,Xu B B,Huang W X,Wang S H,Ding Y F,Liu Z H,Li G H,Chen L,Ding C Q,Tang S.Effect of removing superior spikelets on grain filling of inferior spikelets in rice[J].Frontiers in Plant Science,2016,7:1161.doi:10.3389/fpls.2016.01161.
[26]	张志兴,敏秀梅,宋果,陈花,许海龙,林文雄.14-3-3蛋白在水稻籽粒灌浆过程中的互作靶蛋白鉴定及其对外源激素的响应[J].中国农业科学,2021,54(12):2523-2537.doi:10.3864/j.issn.0578-1752.2021.12.004.
	Zhang Z X,Min X M,Song G,Chen H,Xu H L,Lin W X.Identification of 14-3-3 client proteins in rice grains and their response to exogenous hormones during the grain filling stage[J].Scientia Agricultura Sinica,2021,54(12):2523-2537.
[27]	Zhang H,Li H W,Yuan L M,Wang Z Q,Yang J C,Zhang J H.Post-anthesis alternate wetting and moderate soil drying enhances activities of key enzymes in sucrose-to-starch conversion in inferior spikelets of rice[J].Journal of Experimental Botany,2012,63(1):215-227.doi:10.1093/jxb/err263.
[28]	Jin D M,Wang W J,Lan S Y,Xu Z X,Yang S H.Dynamic status of endogenous IAA,ABA and GA levels in superior and inferior spikelets of heavy panicle hybrid rice during grain filling[J].Journal of Plant Physiology and Molecular Biology,2002,28(3):215-220.doi:10.3321/j.issn:1671-3877.2002.03.010.

引物名称 Primer name	引物序列(5'—3') Primer sequence
miR167a-c_RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTAGATCAT
miR167d-j_RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCAGATCAT
miR1432_RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGTCGGTGT
miR167a-c_F	ACACTCCAGCTGGGTGAAGCTGCCAGCAT
miR167d-j_F	ACACTCCAGCTGGGTGAAGCTGCCAGCAT
miR1432_F	ACACTCCAGCTGGGATCAGGAGAGATGAC
Stem-loop_U	TGGTGTCGTGGAGTCG
OsARF12_F	ACGATGTCACAATGCATGCG
OsARF12_R	AGTAATTCGTCGGCTGCTTG
OsACOT_F	AGGTTGAGGGAAAGCTGTTG
OsACOT_R	GGGCCTGCGCTATCAATTTG
OsGLP3_F	TGTCCAAGGTGCTCAACAAG
OsGLP3_R	TATCCACCTGAAACGCCTTC
OsGF14f_F	AGCAGCTGAGAACACTCTTG
OsGF14f_R	CAGCAATAGCATCGTCGAAC
OsGF14b_F	GCTTGAATCCCACCTTGTC
OsGF14b_R	AATGTCCTGAGCAGCCTTG
OsSUS3_F	CCCCAACTCCGACCTTTACT
OsSUS3_R	TTGCAATCAGGTCAGCTGTG
OsAGPS2_F	GTTCGTTGAAGTCCTCGCTG
OsAGPS2_R	TGCGGTAAAGGTGATCTCCA
OsGBSSI_F	AAGGTTGCAGACAGGTACGA
OsGBSSI_R	TCTTCTCACCGGTCTTTCCC
β-actin_F	GGAAGTACAGTGTCTGGATTGGTG
β-actin_R	TCTTGGCTTAGCATTCTTGGGT

引物名称 Primer name	引物序列(5'—3') Primer sequence
miR167a-c_RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTAGATCAT
miR167d-j_RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCAGATCAT
miR1432_RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGTCGGTGT
miR167a-c_F	ACACTCCAGCTGGGTGAAGCTGCCAGCAT
miR167d-j_F	ACACTCCAGCTGGGTGAAGCTGCCAGCAT
miR1432_F	ACACTCCAGCTGGGATCAGGAGAGATGAC
Stem-loop_U	TGGTGTCGTGGAGTCG
OsARF12_F	ACGATGTCACAATGCATGCG
OsARF12_R	AGTAATTCGTCGGCTGCTTG
OsACOT_F	AGGTTGAGGGAAAGCTGTTG
OsACOT_R	GGGCCTGCGCTATCAATTTG
OsGLP3_F	TGTCCAAGGTGCTCAACAAG
OsGLP3_R	TATCCACCTGAAACGCCTTC
OsGF14f_F	AGCAGCTGAGAACACTCTTG
OsGF14f_R	CAGCAATAGCATCGTCGAAC
OsGF14b_F	GCTTGAATCCCACCTTGTC
OsGF14b_R	AATGTCCTGAGCAGCCTTG
OsSUS3_F	CCCCAACTCCGACCTTTACT
OsSUS3_R	TTGCAATCAGGTCAGCTGTG
OsAGPS2_F	GTTCGTTGAAGTCCTCGCTG
OsAGPS2_R	TGCGGTAAAGGTGATCTCCA
OsGBSSI_F	AAGGTTGCAGACAGGTACGA
OsGBSSI_R	TCTTCTCACCGGTCTTTCCC
β-actin_F	GGAAGTACAGTGTCTGGATTGGTG
β-actin_R	TCTTGGCTTAGCATTCTTGGGT

处理 Treatment	穗数/ (×10⁴/hm²) No.of spikelets	穗粒数 Spikelets per panicle	结实率/% Seed setting percentage	千粒质量/g 1000-grain weight		产量/ (t/hm²) Yield
处理 Treatment	穗数/ (×10⁴/hm²) No.of spikelets	穗粒数 Spikelets per panicle	结实率/% Seed setting percentage	强势籽粒 Superior spikelets	弱势籽粒 Inferior spikelets	产量/ (t/hm²) Yield
CK	299.27±4.97a	284.07±9.39a	82.94±1.01c	27.46±0.30ab	16.93±0.38b	11.14±0.14b
T1	299.31±3.83a	289.48±9.46a	89.90±0.73a	27.87±0.16a	19.65±0.31a	11.70±0.17a
T2	299.25±4.66a	292.79±8.44a	87.00±0.98b	27.13±0.20b	19.62±0.86a	11.65±0.13a

处理 Treatment	穗数/ (×10⁴/hm²) No.of spikelets	穗粒数 Spikelets per panicle	结实率/% Seed setting percentage	千粒质量/g 1000-grain weight		产量/ (t/hm²) Yield
处理 Treatment	穗数/ (×10⁴/hm²) No.of spikelets	穗粒数 Spikelets per panicle	结实率/% Seed setting percentage	强势籽粒 Superior spikelets	弱势籽粒 Inferior spikelets	产量/ (t/hm²) Yield
CK	299.27±4.97a	284.07±9.39a	82.94±1.01c	27.46±0.30ab	16.93±0.38b	11.14±0.14b
T1	299.31±3.83a	289.48±9.46a	89.90±0.73a	27.87±0.16a	19.65±0.31a	11.70±0.17a
T2	299.25±4.66a	292.79±8.44a	87.00±0.98b	27.13±0.20b	19.62±0.86a	11.65±0.13a

处理 Treatment	灌浆起始势 R₀	最大灌浆速率/(mg/d) V_max	平均灌浆速率/(mg/d) V	活跃灌浆期/d AGP
CK	0.073±0.000b	0.568±0.001c	0.364±0.001c	46.361±0.179a
T1	0.078±0.001a	0.708±0.002a	0.449±0.001a	41.332±0.174c
T2	0.077±0.000a	0.658±0.000b	0.419±0.000b	44.029±0.062b

Please choose a citation manager

Content to export