Effects on Glucosinolates and Antioxidant Activity of Chitooligosaccharides on Kale Sprouts

doi:10.7668/hbnxb.20194448

Abstract

Abstract:

In order to investigate the effect of chitooligosaccharides (COS) on the growth of kale sprouts and to provide a reference for the rational application of chitooligosaccharides in the production of sprouts,this study used Jingyu 1 as the test material,and applied different concentrations of COS (25,50,75,100 mg/L) to the seeds after germination to observe the effects of COS on the growth indexes,content of glucosinolates and antioxidant capacity.The results showed that total phenolics content,total flavonoids content,total glucosinolates content,myrosinase enzyme activity and DPPH free radicals scavenging rate of 75 mg/L COS-treated kale sprouts showed an upward trend,and the differences were significant with the control,with increases of 25.99%,17.99%,29.68%,141.88%,and 2.51%,respectively;the yield and total phenolics content,total flavonoids content,sodium content,boron content and myrosinase enzyme activity,of 100 mg/L COS-treated kale sprouts were also enhanced accordingly and differed significantly from the control,increasing by 15.21%,21.29%,22.47%,15.97%,32.63%,97.71%,respectively.After correlation analysis,it could be obtained that the yield of sprouted seedlings was significantly and positively correlated with the content of carotenoids,total phenolics,and total flavonoids and DPPH free radicals scavenging rate.In conclusion,chitosan treatment could increase the yield of kale sprouts,improve the nutritional quality and antioxidant capacity of sprouts,and increase the edible value of sprouts.

Key words: Kale, Chitooligosaccharides, Sprouts, Growth, Antioxidant capacity, Glucosinolates

SHEN Qiaoling, LIU Guangmin, WANG Yaqin, ZHAO Zixuan, WEI Lei, HE Hongju, LIU Haihe. Effects on Glucosinolates and Antioxidant Activity of Chitooligosaccharides on Kale Sprouts[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 104-112. doi: 10.7668/hbnxb.20194448.

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References 36

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处理 Treatments	株高/cm Plant height	单株质量/mg Plant weight	含水率/% Water content
CK	2.48±0.05a	28.54±2.59b	0.92±0.01a
T₁	2.33±0.06a	28.42±2.55b	0.91±0.01a
T₂	2.60±0.19a	29.67±2.70ab	0.91±0.01a
T₃	2.55±0.08a	31.46±0.38ab	0.92±0.00a
T₄	2.56±0.20a	32.88±1.44a	0.92±0.01a

处理 Treatments	株高/cm Plant height	单株质量/mg Plant weight	含水率/% Water content
CK	2.48±0.05a	28.54±2.59b	0.92±0.01a
T₁	2.33±0.06a	28.42±2.55b	0.91±0.01a
T₂	2.60±0.19a	29.67±2.70ab	0.91±0.01a
T₃	2.55±0.08a	31.46±0.38ab	0.92±0.00a
T₄	2.56±0.20a	32.88±1.44a	0.92±0.01a

处理 Treatments	叶绿素a含量/ (mg/g) Chla content	叶绿素b含量/ (mg/g) Chlb content	总叶绿素含量/ (mg/g) Chl(a+b)content	叶绿素a/b Chl(a/b)	类胡萝卜素含量/ (mg/g) Car content
CK	0.32±0.02ab	0.14±0.01a	0.47±0.02ab	2.27±0.04c	5.69±0.22ab
T₁	0.30±0.01b	0.13±0.01a	0.43±0.02bc	2.33±0.04c	5.27±0.24b
T₂	0.34±0.02a	0.13±0.01a	0.47±0.03ab	2.57±0.07b	5.86±0.37a
T₃	0.35±0.01a	0.13±0.01a	0.48±0.02a	2.62±0.16b	6.06±0.23a
T₄	0.30±0.02b	0.11±0.02b	0.41±0.04c	2.85±0.23a	5.35±0.38b

处理 Treatments	叶绿素a含量/ (mg/g) Chla content	叶绿素b含量/ (mg/g) Chlb content	总叶绿素含量/ (mg/g) Chl(a+b)content	叶绿素a/b Chl(a/b)	类胡萝卜素含量/ (mg/g) Car content
CK	0.32±0.02ab	0.14±0.01a	0.47±0.02ab	2.27±0.04c	5.69±0.22ab
T₁	0.30±0.01b	0.13±0.01a	0.43±0.02bc	2.33±0.04c	5.27±0.24b
T₂	0.34±0.02a	0.13±0.01a	0.47±0.03ab	2.57±0.07b	5.86±0.37a
T₃	0.35±0.01a	0.13±0.01a	0.48±0.02a	2.62±0.16b	6.06±0.23a
T₄	0.30±0.02b	0.11±0.02b	0.41±0.04c	2.85±0.23a	5.35±0.38b

处理 Treatments	2-丙烯基硫苷 SIN	3-丁烯基硫苷 NAP	2-羟基-3-丁烯基硫苷 PRO	4-甲硫基丁烯基硫苷 ERU	4-甲基亚磺酰基- 3-丁烯基硫苷 RAE	4-甲基硫氧丁基硫苷 RAA	5-甲基亚磺酰基戊基硫苷 ALY
CK	967.95±46.59ab	64.23±5.31c	577.90±0.01c	86.46±0.31d	0.03±0.04a	289.00±9.11e	6.75±0.60c
T₁	906.00±58.00b	79.74±1.88b	736.83±60.41b	102.59±5.96c	0.09±0.13a	353.62±9.88d	9.64±0.01b
T₂	780.71±8.56c	60.40±2.74c	667.50±18.83b	103.47±1.84c	0.07±0.10a	381.52±0.52c	8.48±0.86bc
T₃	1 014.68±31.76a	114.50±6.76a	847.43±19.00a	161.44±3.75a	0.09±0.13a	492.88±14.38a	14.63±1.27a
T₄	879.88±13.02b	82.84±3.67b	677.06±22.55b	116.48±1.40b	0.01±0.02a	410.95±11.31b	10.14±0.33b

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