Acquirement and Bioassay of Transgenic Insect-resistant Cry1Ab-t Gene Maize YA108

doi:10.7668/hbnxb.20191690

Abstract

Abstract: In order to breeding maize inbred lines with favorite pest resistant.The modified insect resistant gene Cry1Ab-t was constructed into the plant expression vector pCAMBIA3300m and transferred into the hybrid maize HiⅡ immature embryo with the Agrobacterium-mediated method. The positive transgenic plants were obtained by bar screening and PCR detection.RT-PCR, test strip and ELISA methods were used to detect Cry1Ab-t gene expression in transgenic maize,and the insect resistance was evaluated. The results showed that one hundred and sixteen seedlings were obtained after selecting with bialaphos sodium, and eighty-two seedlings of them were positive by PCR analysis and detection of the Cry1Ab-t gene and bar marker gene. RT-PCR, BT-Cry1Ab/Ac test strip and ELISA methods were used to detect Cry1Ab-t gene expression in transgenic material YA108 with good growth, Cry1Ab-t gene was expressed at transcription and translation level. The results of insect identification indicated that the non transgenic maize filaments were seriously infested by corn borer. However, the transgenic maize YA108 had a high level of insect resistance and stable insect resistance. The non transgenic maize was bitten seriously in the late stage of silking, and there were many boreholes in the stem and mold in the wound, while the transgenic maize YA108 was not damaged. After field inoculation, YA108, the Cry1Ab-t transgenic maize, had good resistance to Asian corn borer in both leaves and filaments. It could kill the larvae of Asian corn borer in a short time and reduce the mildew caused by insect pests to a great extent. The results of indoor and field indirect insect identification showed that the resistance rate of YA108 was detected to be Class 1 at each generation, showing high resistance level to Ostrinia furnacalis.

Key words: Transgenic maize, Cry1Ab-t gene, Agrobacterium-mediated method, Molecular identification, Ostrinia furnacalis, Insect-resistant identification

CLC Number:

S513
Q78

YUE Runqing, LIU Lu, TIE Shuanggui, XU Xinzhi, CHEN Nana. Acquirement and Bioassay of Transgenic Insect-resistant Cry1Ab-t Gene Maize YA108[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 212-218. doi: 10.7668/hbnxb.20191690.

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