Study on the Diversity of Endophytic Bacteria in Leaves of Four Potato Varieties in Yunnan Province

doi:10.7668/hbnxb.20192428

Abstract

Abstract: In order to clarify the diversity and differences of endophytic bacteria in leaves of different potato varieties and provide basis for studying the mechanism of potato stress tolerance and the development of endophytic bacteria.The endophytic bacteria from the leaves of four main potato varieties in Yunnan Province were isolated and cultured on LB medium,all those strains were identified based on the morphological characteristics and the 16S rDNA sequence.There were 10 species of endophytic bacteria belonging to 9 genera,8 families and 3 phyla were isolated from Qingshu No.9 leaves. Staphylococcus and Exiguobacterium were the dominant genera,and the relative abundance of both genera were 18.18%.There were 12 species of endophytic bacteria belonging to 11 genera,11 families and 4 phyla were isolated from Hui-2 leaves. Acinetobacter and Pseudomonas were the dominant genera,and the relative abundance of both genera were 15.38%.There were 13 species of endophytic bacteria belonging to 10 genera,9 families and 4 phyla were isolated from Hezuo No.88 leaves. Acinetobacter were the dominant genera,with a relative abundance of 23.53%.There were 17 species of endophytic bacteria belonging to 8 genera,7 families and 4 phyla were isolated from Lishu No.6 leaves. Staphylococcus were the dominant genera,with a relative abundance of 22.22%.There were 37 species of culturable bacteria were isolated from the leaves of four potato varieties.There were 4,8,4,10 unique endophytic bacteria from the leaves of Qingshu No.9,Hui-2,Hezuo No.88 and Lishu No.6,respectively.The species composition and dominant genera of endophytic bacteria in leaves of different potato varieties were different,the results would provide bacteria resources for studying the function of endophytic bacteria in potato leaves.

Key words: Potato varieties, Endophytic bacteria, Molecular identification, Potato leaves, Culturable bacteria

CLC Number:

S532.01

YANG Jinrui, WANG Wenqian, WANG Yue, YAO Yao, JI Ze, LI Junyi, CHEN Bin, XIAO Guanli. Study on the Diversity of Endophytic Bacteria in Leaves of Four Potato Varieties in Yunnan Province[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 178-188. doi: 10.7668/hbnxb.20192428.

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