The Effects of Different Potato Genotypes and Different Inoculation Methods on VIGS Silence Efficiency

doi:10.7668/hbnxb.2014.01.008

Abstract

Abstract: In this study,factors which affect the silence system of VIGS in potato was unravel,the experiment results suggested that the genotype is the main factor to affect the result of VIGS. Among all the tested potato varieties,VIGS showed the highest silence effect in potato variety Wumeng 601,the pds gene expression level was decressed by 79. 8%; and the pds gene expression level in Hutou,Shepody and Atlantic was decreased by 64. 1%, 31. 0% and 4. 5% respectively. The effects of inoculation ways such as leaf injection and stem scrub on the silence result of VIGS was also tested,the results suggested that the correlation between inoculation ways and silence effect was not very strong. In Wumeng 601,the pds gene expression level of leaf injection and stem scrub were decreased by 59. 0% and 56. 5% separately; it is 34. 7% and 40. 3% respectively in potato variety Hutou. The analysis of variance(ANOVA) showed there does not have significant difference between the two inoculation ways.

Key words: VIGS, Silence, Potato varieties, Inoculation method

CLC Number:

Q812
Q78

ZHANG Xiao-luo, ZHANG Zhi-wei, NA Ren, YANG Shu-qing, GAO Jing, ZHAO Jun. The Effects of Different Potato Genotypes and Different Inoculation Methods on VIGS Silence Efficiency[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 36-40. doi: 10.7668/hbnxb.2014.01.008.

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